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@article{1710056,
author = {Veselý, Lukáš and Behera, Susrisweta and Heger, Dominik},
article_location = {Amsterdam},
article_number = {January 2021},
doi = {http://dx.doi.org/10.1016/j.ijpharm.2020.120128},
keywords = {pH shift; Cryopreservation; Stabilization; Freeze-concentrated solution; Sulfonephthalein indicators; Hammett acidity function; Cooling rate},
language = {eng},
issn = {0378-5173},
journal = {International Journal of Pharmaceutics},
title = {Making good’s buffers good for freezing: The acidity changes and their elimination via mixing with sodium phosphate},
url = {https://doi.org/10.1016/j.ijpharm.2020.120128},
volume = {593},
year = {2021}
}
TY - JOUR
ID - 1710056
AU - Veselý, Lukáš - Behera, Susrisweta - Heger, Dominik
PY - 2021
TI - Making good’s buffers good for freezing: The acidity changes and their elimination via mixing with sodium phosphate
JF - International Journal of Pharmaceutics
VL - 593
IS - January 2021
SP - 1-9
EP - 1-9
PB - Elsevier
SN - 03785173
KW - pH shift
KW - Cryopreservation
KW - Stabilization
KW - Freeze-concentrated solution
KW - Sulfonephthalein indicators
KW - Hammett acidity function
KW - Cooling rate
UR - https://doi.org/10.1016/j.ijpharm.2020.120128
L2 - https://doi.org/10.1016/j.ijpharm.2020.120128
N2 - Solutions of three Good’s buffers (HEPES, MOPS, and MES), both pure and mixed with sodium phosphate buffers (Na-P), are investigated in terms of the freezing-induced acidity changes in their operational pH ranges. The Good’s buffers have the tendency to basify upon freezing and, more intensively, at lower pHs. The acidity varies most prominently in MES, where the change may reach the value of two. Importantly, the Good’s buffers are shown to mitigate the strong acidification in the Na-P buffer. Diverse concentrations of the Good’s buffers are added to cancel out the strong, freezing-induced acidity drop in 50 mM Na-P that markedly contributes to the solution’s acidity; the relevant values are 3 mM HEPES, 10 mM MOPS, and 80 mM MES. These buffer blends are therefore proposed to be applied in maintaining approximately the acidity of solutions even after the freezing process and, as such, should limit the stresses for frozen chemicals and biochemicals.
ER -
VESELÝ, Lukáš, Susrisweta BEHERA a Dominik HEGER. Making good’s buffers good for freezing: The acidity changes and their elimination via mixing with sodium phosphate. \textit{International Journal of Pharmaceutics}. Amsterdam: Elsevier, 2021, roč.~593, January 2021, s.~1-9. ISSN~0378-5173. Dostupné z: https://dx.doi.org/10.1016/j.ijpharm.2020.120128.
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