Arabidopsis Flippases Cooperate with ARF GTPase Exchange
Factors to Regulate the Trafficking and Polarity of PIN Auxin
Transporters

J 2020

Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters

ZHANG, Xixi, Maciek ADAMOWSKI, Petra MARHAVA, Shutang TAN, Yuzhou ZHANG et. al.

Základní údaje

Originální název

Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters

Autoři

ZHANG, Xixi, Maciek ADAMOWSKI, Petra MARHAVA, Shutang TAN, Yuzhou ZHANG, Lesia RODRIGUEZ, Marta ZWIEWKA (616 Polsko, domácí), Vendula PUKYŠOVÁ (203 Česká republika, domácí), Asier SANCHEZ CERDAN (724 Španělsko, domácí), Vivek Kumar RAXWAL (356 Indie, domácí), Christian S. HARDTKE, Tomasz NODZYNSKI (616 Polsko, garant, domácí) a Jiří FRIML

Vydání

Plant Cell, Rockville, American Society of Plant Biologists, 2020, 1040-4651

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 11.277

Kód RIV

RIV/00216224:14740/20:00114574

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1105/tpc.19.00869

UT WoS

000545741500030

Klíčová slova anglicky

P-TYPE ATPASE; TRANS-GOLGI NETWORK; APICAL-BASAL AXIS; GENE-EXPRESSION; PHOSPHOLIPID FLIPPASES; MEDIATES ENDOCYTOSIS; MEMBRANE TRAFFICKING; VACUOLAR TRAFFICKING; PROTEIN TRAFFICKING; BETA-SUBUNIT

Štítky

CF CELLIM, CF PLANT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 10. 2024 09:27, Ing. Martina Blahová

Anotace

V originále

Cell polarity is a fundamental feature of all multicellular organisms. PIN auxin transporters are important cell polarity markers that play crucial roles in a plethora of developmental processes in plants. Here, to identify components involved in cell polarity establishment and maintenance in plants, we performed a forward genetic screening of PIN2:PIN1-HA;pin2 Arabidopsis (Arabidopsis thaliana) plants, which ectopically express predominantly basally localized PIN1 in root epidermal cells, leading to agravitropic root growth. We identified the regulator of PIN polarity 12 (repp12) mutation, which restored gravitropic root growth and caused a switch in PIN1-HA polarity from the basal to apical side of root epidermal cells. Next Generation Sequencing and complementation experiments established the causative mutation of repp12 as a single amino acid exchange in Aminophospholipid ATPase3 (ALA3), a phospholipid flippase predicted to function in vesicle formation. repp12 and ala3 T-DNA mutants show defects in many auxin-regulated processes, asymmetric auxin distribution, and PIN trafficking. Analysis of quintuple and sextuple mutants confirmed the crucial roles of ALA proteins in regulating plant development as well as PIN trafficking and polarity. Genetic and physical interaction studies revealed that ALA3 functions together with the ADP ribosylation factor GTPase exchange factors GNOM and BIG3 in regulating PIN polarity, trafficking, and auxin-mediated development.

Návaznosti

EF15_003/0000479, projekt VaV

Název: Regulace rostlinné meiózy

GA18-26981S, projekt VaV

Název: Genetické studie k objasnění molekulárního mechanizmu účinku strigolaktonů v kořeni

Investor: Grantová agentura ČR, Genetické studie k objasnění molekulárního mechanizmu účinku strigolaktonů v kořeni

GJ17-17966Y, projekt VaV

Název: Charakteristika nových molekulárních komponentů podílejících se na distribuci proteinů v rostlinách.

90129, velká výzkumná infrastruktura

Název: Czech-BioImaging II

Citovat

ZHANG, Xixi, Maciek ADAMOWSKI, Petra MARHAVA, Shutang TAN, Yuzhou ZHANG, Lesia RODRIGUEZ, Marta ZWIEWKA, Vendula PUKYŠOVÁ, Asier SANCHEZ CERDAN, Vivek Kumar RAXWAL, Christian S. HARDTKE, Tomasz NODZYNSKI a Jiří FRIML. Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters. Plant Cell. Rockville: American Society of Plant Biologists, 2020, roč. 32, č. 5, s. 1644-1664. ISSN 1040-4651. Dostupné z: https://dx.doi.org/10.1105/tpc.19.00869.

@article{1710076,
   author = {Zhang, Xixi and Adamowski, Maciek and Marhava, Petra and Tan, Shutang and Zhang, Yuzhou and Rodriguez, Lesia and Zwiewka, Marta and Pukyšová, Vendula and Sanchez Cerdan, Asier and Raxwal, Vivek Kumar and Hardtke, Christian S. and Nodzynski, Tomasz and Friml, Jiří},
   article_location = {Rockville},
   article_number = {5},
   doi = {http://dx.doi.org/10.1105/tpc.19.00869},
   keywords = {P-TYPE ATPASE; TRANS-GOLGI NETWORK; APICAL-BASAL AXIS; GENE-EXPRESSION; PHOSPHOLIPID FLIPPASES; MEDIATES ENDOCYTOSIS; MEMBRANE TRAFFICKING; VACUOLAR TRAFFICKING; PROTEIN TRAFFICKING; BETA-SUBUNIT},
   language = {eng},
   issn = {1040-4651},
   journal = {Plant Cell},
   title = {Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters},
   url = {https://doi.org/10.1105/tpc.19.00869},
   volume = {32},
   year = {2020}
}

TY  - JOUR
ID  - 1710076
AU  - Zhang, Xixi - Adamowski, Maciek - Marhava, Petra - Tan, Shutang - Zhang, Yuzhou - Rodriguez, Lesia - Zwiewka, Marta - Pukyšová, Vendula - Sanchez Cerdan, Asier - Raxwal, Vivek Kumar - Hardtke, Christian S. - Nodzynski, Tomasz - Friml, Jiří
PY  - 2020
TI  - Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters
JF  - Plant Cell
VL  - 32
IS  - 5
SP  - 1644-1664
EP  - 1644-1664
PB  - American Society of Plant Biologists
SN  - 10404651
KW  - P-TYPE ATPASE
KW  - TRANS-GOLGI NETWORK
KW  - APICAL-BASAL AXIS
KW  - GENE-EXPRESSION
KW  - PHOSPHOLIPID FLIPPASES
KW  - MEDIATES ENDOCYTOSIS
KW  - MEMBRANE TRAFFICKING
KW  - VACUOLAR TRAFFICKING
KW  - PROTEIN TRAFFICKING
KW  - BETA-SUBUNIT
UR  - https://doi.org/10.1105/tpc.19.00869
L2  - https://doi.org/10.1105/tpc.19.00869
N2  - Cell polarity is a fundamental feature of all multicellular organisms. PIN auxin transporters are important cell polarity markers that play crucial roles in a plethora of developmental processes in plants. Here, to identify components involved in cell polarity establishment and maintenance in plants, we performed a forward genetic screening of PIN2:PIN1-HA;pin2 Arabidopsis (Arabidopsis thaliana) plants, which ectopically express predominantly basally localized PIN1 in root epidermal cells, leading to agravitropic root growth. We identified the regulator of PIN polarity 12 (repp12) mutation, which restored gravitropic root growth and caused a switch in PIN1-HA polarity from the basal to apical side of root epidermal cells. Next Generation Sequencing and complementation experiments established the causative mutation of repp12 as a single amino acid exchange in Aminophospholipid ATPase3 (ALA3), a phospholipid flippase predicted to function in vesicle formation. repp12 and ala3 T-DNA mutants show defects in many auxin-regulated processes, asymmetric auxin distribution, and PIN trafficking. Analysis of quintuple and sextuple mutants confirmed the crucial roles of ALA proteins in regulating plant development as well as PIN trafficking and polarity. Genetic and physical interaction studies revealed that ALA3 functions together with the ADP ribosylation factor GTPase exchange factors GNOM and BIG3 in regulating PIN polarity, trafficking, and auxin-mediated development.
ER  -

ZHANG, Xixi, Maciek ADAMOWSKI, Petra MARHAVA, Shutang TAN, Yuzhou ZHANG, Lesia RODRIGUEZ, Marta ZWIEWKA, Vendula PUKYŠOVÁ, Asier SANCHEZ CERDAN, Vivek Kumar RAXWAL, Christian S. HARDTKE, Tomasz NODZYNSKI a Jiří FRIML. Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters. \textit{Plant Cell}. Rockville: American Society of Plant Biologists, 2020, roč.~32, č.~5, s.~1644-1664. ISSN~1040-4651. Dostupné z: https://dx.doi.org/10.1105/tpc.19.00869.

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