Conducting composite films based on chitosan or sodium
hyaluronate. Properties and cytocompatibility with human
induced pluripotent stem cells

J 2021

Conducting composite films based on chitosan or sodium hyaluronate. Properties and cytocompatibility with human induced pluripotent stem cells

JASENSKÁ, Daniela; Věra KAŠPÁRKOVÁ; Katarzyna Anna RADASZKIEWICZ; Zdenka CAPÁKOVÁ; Jiří PACHERNÍK et. al.

Basic information

Original name

Conducting composite films based on chitosan or sodium hyaluronate. Properties and cytocompatibility with human induced pluripotent stem cells

Authors

JASENSKÁ, Daniela; Věra KAŠPÁRKOVÁ; Katarzyna Anna RADASZKIEWICZ (616 Poland, belonging to the institution); Zdenka CAPÁKOVÁ; Jiří PACHERNÍK (203 Czech Republic, belonging to the institution); Miroslava TRCHOVÁ; Antonín MINAŘÍK; Ján VAJĎÁK; Tomáš BÁRTA (203 Czech Republic, belonging to the institution); Jaroslav STEJSKAL; Marián LEHOCKÝ; Thanh Huong TRUONG; Robert MOUČKA and Petr HUMPOLÍČEK

Edition

Carbohydrate Polymers, Oxford, Elsevier, 2021, 0144-8617

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10601 Cell biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 10.723

RIV identification code

RIV/00216224:14310/21:00118800

Organization unit

Faculty of Science

DOI

https://doi.org/10.1016/j.carbpol.2020.117244

UT WoS

000596248700003

EID Scopus

2-s2.0-85094181117

Keywords in English

Polyaniline; Polysacharides; Conducting composites; Human induced pluripotent stem cells

Abstract

In the original language

Novel composite films combining biocompatible polysaccharides with conducting polyaniline (PANI) were prepared via the in-situ polymerization of aniline hydrochloride in the presence of sodium hyaluronate (SH) or chitosan (CH). The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC). Moreover, the cardiomyogenesis and even formation of beating clusters were successfully induced on the films. The proportion of formed cardiomyocytes demonstrated excellent properties of composites for tissue engineering of stimuli-responsive tissues. The testing also demonstrated antibacterial activity of the films against E. coli and PANI-SH was able to reduce bacterial growth from 2 x 10(5) to < 1 cfu cm(-2). Physicochemical characterization revealed that the presence of polysaccharides did not notably influence conductivities of the composites being similar to 1 and similar to 2 S cm(-1) for PANI-SH and PANI-CH respectively; however, in comparison with neat PANI, it modified their topography making the films smoother with mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH). The combination of conductivity, antibacterial activity and mainly cytocompatibility with hiPSC opens wide application potential of these polysaccharide-based composites.

Links

GA18-18235S, research and development project

Name: Nové možnosti v kardiomyogenní diferenciaci pluripotentních kmenových buněk (Acronym: KarPKB)

Investor: Czech Science Foundation

GA19-16861S, research and development project

Name: Interakce biomateriálů s kmenovými buňkami v simulovaných in vivo podmínkách (Acronym: IBMSKB)

Investor: Czech Science Foundation

Cite

JASENSKÁ, Daniela; Věra KAŠPÁRKOVÁ; Katarzyna Anna RADASZKIEWICZ; Zdenka CAPÁKOVÁ; Jiří PACHERNÍK; Miroslava TRCHOVÁ; Antonín MINAŘÍK; Ján VAJĎÁK; Tomáš BÁRTA; Jaroslav STEJSKAL; Marián LEHOCKÝ; Thanh Huong TRUONG; Robert MOUČKA and Petr HUMPOLÍČEK. Conducting composite films based on chitosan or sodium hyaluronate. Properties and cytocompatibility with human induced pluripotent stem cells. Carbohydrate Polymers. Oxford: Elsevier, 2021, vol. 253, February 2021, p. 1-10. ISSN 0144-8617. Available from: https://doi.org/10.1016/j.carbpol.2020.117244.

@article{1710516,
   author = {Jasenská, Daniela and Kašpárková, Věra and Radaszkiewicz, Katarzyna Anna and Capáková, Zdenka and Pacherník, Jiří and Trchová, Miroslava and Minařík, Antonín and Vajďák, Ján and Bárta, Tomáš and Stejskal, Jaroslav and Lehocký, Marián and Truong, Thanh Huong and Moučka, Robert and Humpolíček, Petr},
   article_location = {Oxford},
   article_number = {February 2021},
   doi = {https://doi.org/10.1016/j.carbpol.2020.117244},
   keywords = {Polyaniline; Polysacharides; Conducting composites; Human induced pluripotent stem cells},
   language = {eng},
   issn = {0144-8617},
   journal = {Carbohydrate Polymers},
   title = {Conducting composite films based on chitosan or sodium hyaluronate. Properties and cytocompatibility with human induced pluripotent stem cells},
   url = {https://doi.org/10.1016/j.carbpol.2020.117244},
   volume = {253},
   year = {2021}
}

TY  - JOUR
ID  - 1710516
AU  - Jasenská, Daniela - Kašpárková, Věra - Radaszkiewicz, Katarzyna Anna - Capáková, Zdenka - Pacherník, Jiří - Trchová, Miroslava - Minařík, Antonín - Vajďák, Ján - Bárta, Tomáš - Stejskal, Jaroslav - Lehocký, Marián - Truong, Thanh Huong - Moučka, Robert - Humpolíček, Petr
PY  - 2021
TI  - Conducting composite films based on chitosan or sodium hyaluronate. Properties and cytocompatibility with human induced pluripotent stem cells
JF  - Carbohydrate Polymers
VL  - 253
IS  - February 2021
SP  - 1-10
EP  - 1-10
PB  - Elsevier
SN  - 01448617
KW  - Polyaniline
KW  - Polysacharides
KW  - Conducting composites
KW  - Human induced pluripotent stem cells
UR  - https://doi.org/10.1016/j.carbpol.2020.117244
L2  - https://doi.org/10.1016/j.carbpol.2020.117244
N2  - Novel composite films combining biocompatible polysaccharides with conducting polyaniline (PANI) were prepared via the in-situ polymerization of aniline hydrochloride in the presence of sodium hyaluronate (SH) or chitosan (CH). The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC). Moreover, the cardiomyogenesis and even formation of beating clusters were successfully induced on the films. The proportion of formed cardiomyocytes demonstrated excellent properties of composites for tissue engineering of stimuli-responsive tissues. The testing also demonstrated antibacterial activity of the films against E. coli and PANI-SH was able to reduce bacterial growth from 2 x 10(5) to < 1 cfu cm(-2). Physicochemical characterization revealed that the presence of polysaccharides did not notably influence conductivities of the composites being similar to 1 and similar to 2 S cm(-1) for PANI-SH and PANI-CH respectively; however, in comparison with neat PANI, it modified their topography making the films smoother with mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH). The combination of conductivity, antibacterial activity and mainly cytocompatibility with hiPSC opens wide application potential of these polysaccharide-based composites.
ER  -

JASENSKÁ, Daniela; Věra KAŠPÁRKOVÁ; Katarzyna Anna RADASZKIEWICZ; Zdenka CAPÁKOVÁ; Jiří PACHERNÍK; Miroslava TRCHOVÁ; Antonín MINAŘÍK; Ján VAJĎÁK; Tomáš BÁRTA; Jaroslav STEJSKAL; Marián LEHOCKÝ; Thanh Huong TRUONG; Robert MOUČKA and Petr HUMPOLÍČEK. Conducting composite films based on chitosan or sodium hyaluronate. Properties and cytocompatibility with human induced pluripotent stem cells. \textit{Carbohydrate Polymers}. Oxford: Elsevier, 2021, vol.~253, February 2021, p.~1-10. ISSN~0144-8617. Available from: https://doi.org/10.1016/j.carbpol.2020.117244.

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