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@inbook{1712256, author = {Marvanová, Ludmila}, address = {Cham}, booktitle = {Methods to Study Litter Decomposition}, doi = {http://dx.doi.org/10.1007/978-3-030-30515-4_24}, edition = {2}, editor = {Felix Bärlocher; Mark O. Gessner; Manuel A.S. Graça}, keywords = {Culture storage; Deionized water preservation; Fungal culture preservation; Fungal genetic resources; Aquatic fungi; Liquid nitrogen preservation; Pure culture databases; Pure cultures; World culture collections}, howpublished = {tištěná verze "print"}, language = {eng}, location = {Cham}, isbn = {978-3-030-30514-7}, pages = {211-222}, publisher = {Springer}, title = {Maintenance of Aquatic Hyphomycete Cultures}, url = {https://doi.org/10.1007/978-3-030-30515-4_24}, year = {2020} }
TY - CHAP ID - 1712256 AU - Marvanová, Ludmila PY - 2020 TI - Maintenance of Aquatic Hyphomycete Cultures VL - Neuveden PB - Springer CY - Cham SN - 9783030305147 KW - Culture storage KW - Deionized water preservation KW - Fungal culture preservation KW - Fungal genetic resources KW - Aquatic fungi KW - Liquid nitrogen preservation KW - Pure culture databases KW - Pure cultures KW - World culture collections UR - https://doi.org/10.1007/978-3-030-30515-4_24 L2 - https://doi.org/10.1007/978-3-030-30515-4_24 N2 - Pure cultures of fungi are very broadly used in various human activities, including medicine and various branches of industry. Moreover, some characters required for the identification of fungi decomposing leaf litter in streams can be studied only in pure culture. This chapter presents methods to keep fungal pure cultures viable and possibly genetically unchanged while avoiding contamination. The principle is to keep cultures in conditions that reduce or even suspend metabolism. The simplest and least expensive method is preservation of pieces of agar culture in small bottles with sterile deionized water at 10 °C. This approach is suitable for short-term preservation of 2-5 years. Keeping well grown cultures on agar slants flooded with mineral oil in test tubes extends the shelf life to ca. 10 years. These methods do not require special equipment. Most effective, however, is preservation by cryoconservation under suspended metabolism in a deep freezer at –80 °C, or in liquid nitrogen at up to –196 °C. Cryoconservation ensures survival for up to ca. 40 years in the case of deep freezing and practically forever in liquid nitrogen. ER -
MARVANOVÁ, Ludmila. Maintenance of Aquatic Hyphomycete Cultures. In Felix Bärlocher; Mark O. Gessner; Manuel A.S. Gra\c ca. \textit{Methods to Study Litter Decomposition}. 2. vyd. Cham: Springer, 2020, s.~211-222. ISBN~978-3-030-30514-7. Dostupné z: https://dx.doi.org/10.1007/978-3-030-30515-4\_{}24.
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