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@article{1717657,
author = {Kučera, Jiří and Lochman, Jan and Bouchal, Pavel and Pakostova, Eva and Mikulášek, Kamil and Hedrich, Sabrina and Janiczek, Oldřich and Mandl, Martin and Johnson, D. Barrie},
article_location = {Lausanne},
article_number = {November 2020},
doi = {http://dx.doi.org/10.3389/fmicb.2020.610836},
keywords = {Acidithiobacillus; extremophiles; ferric iron reduction; hydrogen metabolism; multi-omics; oxygen reduction},
language = {eng},
issn = {1664-302X},
journal = {Frontiers in Microbiology},
title = {A Model of Aerobic and Anaerobic Metabolism of Hydrogen in the Extremophile Acidithiobacillus ferrooxidans},
url = {https://doi.org/10.3389/fmicb.2020.610836},
volume = {11},
year = {2020}
}
TY - JOUR
ID - 1717657
AU - Kučera, Jiří - Lochman, Jan - Bouchal, Pavel - Pakostova, Eva - Mikulášek, Kamil - Hedrich, Sabrina - Janiczek, Oldřich - Mandl, Martin - Johnson, D. Barrie
PY - 2020
TI - A Model of Aerobic and Anaerobic Metabolism of Hydrogen in the Extremophile Acidithiobacillus ferrooxidans
JF - Frontiers in Microbiology
VL - 11
IS - November 2020
SP - 610836
EP - 610836
PB - Frontiers Media SA
SN - 1664302X
KW - Acidithiobacillus
KW - extremophiles
KW - ferric iron reduction
KW - hydrogen metabolism
KW - multi-omics
KW - oxygen reduction
UR - https://doi.org/10.3389/fmicb.2020.610836
L2 - https://doi.org/10.3389/fmicb.2020.610836
N2 - Hydrogen can serve as an electron donor for chemolithotrophic acidophiles, especially in the deep terrestrial subsurface and geothermal ecosystems. Nevertheless, the current knowledge of hydrogen utilization by mesophilic acidophiles is minimal. A multi-omics analysis was applied on Acidithiobacillus ferrooxidans growing on hydrogen, and a respiratory model was proposed. In the model, [NiFe] hydrogenases oxidize hydrogen to two protons and two electrons. The electrons are used to reduce membrane-soluble ubiquinone to ubiquinol. Genetically associated iron-sulfur proteins mediate electron relay from the hydrogenases to the ubiquinone pool. Under aerobic conditions, reduced ubiquinol transfers electrons to either cytochrome aa(3) oxidase via cytochrome bc(1) complex and cytochrome c(4) or the alternate directly to cytochrome bd oxidase, resulting in proton efflux and reduction of oxygen. Under anaerobic conditions, reduced ubiquinol transfers electrons to outer membrane cytochrome c (ferrireductase) via cytochrome bc(1) complex and a cascade of electron transporters (cytochrome c(4), cytochrome c(552), rusticyanin, and high potential iron-sulfur protein), resulting in proton efflux and reduction of ferric iron. The proton gradient generated by hydrogen oxidation maintains the membrane potential and allows the generation of ATP and NADH. These results further clarify the role of extremophiles in biogeochemical processes and their impact on the composition of the deep terrestrial subsurface.
ER -
KUČERA, Jiří, Jan LOCHMAN, Pavel BOUCHAL, Eva PAKOSTOVA, Kamil MIKULÁŠEK, Sabrina HEDRICH, Oldřich JANICZEK, Martin MANDL and D. Barrie JOHNSON. A Model of Aerobic and Anaerobic Metabolism of Hydrogen in the Extremophile Acidithiobacillus ferrooxidans. \textit{Frontiers in Microbiology}. Lausanne: Frontiers Media SA, 2020, vol.~11, November 2020, p.~610836-610849. ISSN~1664-302X. Available from: https://dx.doi.org/10.3389/fmicb.2020.610836.
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