Comparison of Dietary Oils with Different Polyunsaturated Fatty
Acid n-3 and n-6 Content in the Rat Model of Cutaneous Wound
Healing

J 2020

Comparison of Dietary Oils with Different Polyunsaturated Fatty Acid n-3 and n-6 Content in the Rat Model of Cutaneous Wound Healing

KOMPRDA, Tomáš, Zbyšek SLÁDEK, Zuzana ŠEVČÍKOVÁ, Veronika ŠVEHLOVÁ, JanJ WIJACKI et. al.

Základní údaje

Originální název

Comparison of Dietary Oils with Different Polyunsaturated Fatty Acid n-3 and n-6 Content in the Rat Model of Cutaneous Wound Healing

Autoři

KOMPRDA, Tomáš, Zbyšek SLÁDEK, Zuzana ŠEVČÍKOVÁ, Veronika ŠVEHLOVÁ, JanJ WIJACKI, Roman GURÁŇ, TTomáš DO, Zuzana LACKOVÁ, Hana HOLCOVÁ POLANSKÁ (203 Česká republika, domácí), Lucie VRLÍKOVÁ, Vendula POPELKOVÁ, Petr MICHÁLEK, Ondřej ZITKA a Marcela BUCHTOVÁ (203 Česká republika, domácí)

Vydání

International Journal of Molecular Sciences, Basel, MDPI, 2020, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.923

Kód RIV

RIV/00216224:14310/20:00117555

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/ijms21217911

UT WoS

000588974300001

Klíčová slova anglicky

polyunsaturated fatty acids; hydroxyproline; collagen I; III; MPO; macrophages; mast cells; matrix-assisted-laser-desorption-ionization-mass-spectrometry-imaging

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 2. 2023 12:22, Mgr. Marie Šípková, DiS.

Anotace

V originále

Dietary supplementation with polyunsaturated fatty acids (PUFA) n-3 can affect cutaneous wound healing; however, recent findings demonstrate the variable extent of their influence on the quality of healing. Here, we compare the effect of several dietary oils, containing different levels of PUFA n-3 and PUFA n-6, on wound healing in the rat model. Rats were fed the feed mixture with 8% palm oil (P), safflower oil (S), fish oil (F) or Schizochytrium microalga extract (Sch) and compared to the animals fed by control feed mixture (C). Dorsal full-thickness cutaneous excisions were performed after 52 days of feeding and skin was left to heal for an additional 12 days. Histopathological analysis of skin wounds was performed, including immune cells immunolabeling and the determination of hydroxyproline amount as well as gene expression analyses of molecules contributing to different steps of the healing. Matrix-assisted-laser-desorption-ionization mass-spectrometry-imaging (MALDI-MSI) was used to determine the amount of collagen alpha-1(III) chain fragment in healing samples. Treatment by Schizochytrium extract resulted in decrease in the total wound area, in contrast to the safflower oil group where the size of the wound was larger when comparing to control animals. Diet with Schizochytrium extract and safflower oils displayed a tendency to increase the number of new vessels. The number of MPO-positive cells was diminished following any of oil treatment in comparison to the control, but their highest amount was found in animals with a fish oil diet. On the other hand, the number of CD68-positive macrophages was increased, with the most significant enhancement in the fish oil and safflower oil group. Hydroxyproline concentration was the highest in the safflower oil group but it was also enhanced in all other analyzed treatments in comparison to the control. MALDI-MSI signal intensity of a collagen III fragment decreased in the sequence C > S > Sch > P > F treatment. In conclusion, we observed differences in tissue response during healing between dietary oils, with the activation of inflammation observed following the treatment with oil containing high eicosapentaenoic acid (EPA) level (fish oil) and enhanced healing features were induced by the diet with high content of docosahexaenoic acid (DHA, Schizochytrium extract).

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

KOMPRDA, Tomáš, Zbyšek SLÁDEK, Zuzana ŠEVČÍKOVÁ, Veronika ŠVEHLOVÁ, JanJ WIJACKI, Roman GURÁŇ, TTomáš DO, Zuzana LACKOVÁ, Hana HOLCOVÁ POLANSKÁ, Lucie VRLÍKOVÁ, Vendula POPELKOVÁ, Petr MICHÁLEK, Ondřej ZITKA a Marcela BUCHTOVÁ. Comparison of Dietary Oils with Different Polyunsaturated Fatty Acid n-3 and n-6 Content in the Rat Model of Cutaneous Wound Healing. International Journal of Molecular Sciences. Basel: MDPI, 2020, roč. 21, č. 21, s. 1-22. ISSN 1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms21217911.

@article{1718977,
   author = {Komprda, Tomáš and Sládek, Zbyšek and Ševčíková, Zuzana and Švehlová, Veronika and Wijacki, JanJ and Guráň, Roman and Do, TTomáš and Lacková, Zuzana and Holcová Polanská, Hana and Vrlíková, Lucie and Popelková, Vendula and Michálek, Petr and Zitka, Ondřej and Buchtová, Marcela},
   article_location = {Basel},
   article_number = {21},
   doi = {http://dx.doi.org/10.3390/ijms21217911},
   keywords = {polyunsaturated fatty acids; hydroxyproline; collagen I; III; MPO; macrophages; mast cells; matrix-assisted-laser-desorption-ionization-mass-spectrometry-imaging},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Comparison of Dietary Oils with Different Polyunsaturated Fatty Acid n-3 and n-6 Content in the Rat Model of Cutaneous Wound Healing},
   url = {https://www.mdpi.com/1422-0067/21/21/7911},
   volume = {21},
   year = {2020}
}

TY  - JOUR
ID  - 1718977
AU  - Komprda, Tomáš - Sládek, Zbyšek - Ševčíková, Zuzana - Švehlová, Veronika - Wijacki, JanJ - Guráň, Roman - Do, TTomáš - Lacková, Zuzana - Holcová Polanská, Hana - Vrlíková, Lucie - Popelková, Vendula - Michálek, Petr - Zitka, Ondřej - Buchtová, Marcela
PY  - 2020
TI  - Comparison of Dietary Oils with Different Polyunsaturated Fatty Acid n-3 and n-6 Content in the Rat Model of Cutaneous Wound Healing
JF  - International Journal of Molecular Sciences
VL  - 21
IS  - 21
SP  - 1-22
EP  - 1-22
PB  - MDPI
SN  - 14220067
KW  - polyunsaturated fatty acids
KW  - hydroxyproline
KW  - collagen I
KW  - III
KW  - MPO
KW  - macrophages
KW  - mast cells
KW  - matrix-assisted-laser-desorption-ionization-mass-spectrometry-imaging
UR  - https://www.mdpi.com/1422-0067/21/21/7911
L2  - https://www.mdpi.com/1422-0067/21/21/7911
N2  - Dietary supplementation with polyunsaturated fatty acids (PUFA) n-3 can affect cutaneous wound healing; however, recent findings demonstrate the variable extent of their influence on the quality of healing. Here, we compare the effect of several dietary oils, containing different levels of PUFA n-3 and PUFA n-6, on wound healing in the rat model. Rats were fed the feed mixture with 8% palm oil (P), safflower oil (S), fish oil (F) or Schizochytrium microalga extract (Sch) and compared to the animals fed by control feed mixture (C). Dorsal full-thickness cutaneous excisions were performed after 52 days of feeding and skin was left to heal for an additional 12 days. Histopathological analysis of skin wounds was performed, including immune cells immunolabeling and the determination of hydroxyproline amount as well as gene expression analyses of molecules contributing to different steps of the healing. Matrix-assisted-laser-desorption-ionization mass-spectrometry-imaging (MALDI-MSI) was used to determine the amount of collagen alpha-1(III) chain fragment in healing samples. Treatment by Schizochytrium extract resulted in decrease in the total wound area, in contrast to the safflower oil group where the size of the wound was larger when comparing to control animals. Diet with Schizochytrium extract and safflower oils displayed a tendency to increase the number of new vessels. The number of MPO-positive cells was diminished following any of oil treatment in comparison to the control, but their highest amount was found in animals with a fish oil diet. On the other hand, the number of CD68-positive macrophages was increased, with the most significant enhancement in the fish oil and safflower oil group. Hydroxyproline concentration was the highest in the safflower oil group but it was also enhanced in all other analyzed treatments in comparison to the control. MALDI-MSI signal intensity of a collagen III fragment decreased in the sequence C > S > Sch > P > F treatment. In conclusion, we observed differences in tissue response during healing between dietary oils, with the activation of inflammation observed following the treatment with oil containing high eicosapentaenoic acid (EPA) level (fish oil) and enhanced healing features were induced by the diet with high content of docosahexaenoic acid (DHA, Schizochytrium extract).
ER  -

KOMPRDA, Tomáš, Zbyšek SLÁDEK, Zuzana ŠEVČÍKOVÁ, Veronika ŠVEHLOVÁ, JanJ WIJACKI, Roman GURÁŇ, TTomáš DO, Zuzana LACKOVÁ, Hana HOLCOVÁ POLANSKÁ, Lucie VRLÍKOVÁ, Vendula POPELKOVÁ, Petr MICHÁLEK, Ondřej ZITKA a Marcela BUCHTOVÁ. Comparison of Dietary Oils with Different Polyunsaturated Fatty Acid n-3 and n-6 Content in the Rat Model of Cutaneous Wound Healing. \textit{International Journal of Molecular Sciences}. Basel: MDPI, 2020, roč.~21, č.~21, s.~1-22. ISSN~1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms21217911.

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