Slug-expressing mouse prostate epithelial cells have increased
stem cell potential

J 2020

Slug-expressing mouse prostate epithelial cells have increased stem cell potential

KAHOUNOVÁ, Zuzana, Ján REMŠÍK, Radek FEDR, Jan BOUCHAL, Alena MIČKOVÁ et. al.

Základní údaje

Originální název

Slug-expressing mouse prostate epithelial cells have increased stem cell potential

Autoři

KAHOUNOVÁ, Zuzana, Ján REMŠÍK (703 Slovensko, domácí), Radek FEDR, Jan BOUCHAL, Alena MIČKOVÁ, Eva SLABÁKOVÁ, Lucia BINÓ, Aleš HAMPL (203 Česká republika, domácí) a Karel SOUČEK (203 Česká republika, domácí)

Vydání

Stem cell research, Amsterdam, Elsevier, 2020, 1873-5061

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.020

Kód RIV

RIV/00216224:14310/20:00117616

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.scr.2020.101844

UT WoS

000566516300010

Klíčová slova anglicky

Prostate stern cells; Epithelial-to-mesenchymal transition; Snai2/Slug; Organoids; Sternness

Štítky

14110517, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 3. 2021 13:05, Mgr. Marie Šípková, DiS.

Anotace

V originále

Deciphering the properties of adult stem cells is crucial for understanding of their role in healthy tissue and in cancer progression as well. Both stem cells and cancer stem cells have shown association with epithelial-to-mesenchymal transition (EMT) in various tissue types. Aiming to investigate the epithelial and mesenchymal phenotypic traits in adult mouse prostate, we sorted subpopulations of basal prostate stem cells (mPSCs) and assessed the expression levels of EMT regulators and markers with custom-designed gene expression array. The population of mPSCs defined by a Lin(-)/Sca-1(+) CD49f(hi)/Trop-2(+) (LSC Trop-(2+)) surface phenotype was enriched in mesenchymal markers, especially EMT master regulator Slug, encoded by the Snail gene. To further dissect the role of Slug in mPSCs, we used transgenic Snai2(tm1.1wbg) reporter mouse strain. Using this model, we confirmed the presence of mesenchymal traits and increase of organoid forming capacity in Slug(+) population of mPSCs. The Slug(+) -derived organoids comprised all prostate epithelial cell types - basal, luminal, and neuroendocrine. Collectively, these data uncover the important role of Slug expression in the physiology of mouse prostate stem cells.

Návaznosti

EE2.3.20.0185, projekt VaV

Název: Centrum analýz a modelování tkání a orgánů

EE2.4.31.0245, projekt VaV

Název: OrganoNET - partnerství pro vzdělávání a výzkum v oblasti zobrazování tkání a orgánů

Citovat

KAHOUNOVÁ, Zuzana, Ján REMŠÍK, Radek FEDR, Jan BOUCHAL, Alena MIČKOVÁ, Eva SLABÁKOVÁ, Lucia BINÓ, Aleš HAMPL a Karel SOUČEK. Slug-expressing mouse prostate epithelial cells have increased stem cell potential. Stem cell research. Amsterdam: Elsevier, 2020, roč. 46, July 2020, s. 1-10. ISSN 1873-5061. Dostupné z: https://dx.doi.org/10.1016/j.scr.2020.101844.

@article{1721716,
   author = {Kahounová, Zuzana and Remšík, Ján and Fedr, Radek and Bouchal, Jan and Mičková, Alena and Slabáková, Eva and Binó, Lucia and Hampl, Aleš and Souček, Karel},
   article_location = {Amsterdam},
   article_number = {July 2020},
   doi = {http://dx.doi.org/10.1016/j.scr.2020.101844},
   keywords = {Prostate stern cells; Epithelial-to-mesenchymal transition; Snai2/Slug; Organoids; Sternness},
   language = {eng},
   issn = {1873-5061},
   journal = {Stem cell research},
   title = {Slug-expressing mouse prostate epithelial cells have increased stem cell potential},
   url = {https://doi.org/10.1016/j.scr.2020.101844},
   volume = {46},
   year = {2020}
}

TY  - JOUR
ID  - 1721716
AU  - Kahounová, Zuzana - Remšík, Ján - Fedr, Radek - Bouchal, Jan - Mičková, Alena - Slabáková, Eva - Binó, Lucia - Hampl, Aleš - Souček, Karel
PY  - 2020
TI  - Slug-expressing mouse prostate epithelial cells have increased stem cell potential
JF  - Stem cell research
VL  - 46
IS  - July 2020
SP  - 1-10
EP  - 1-10
PB  - Elsevier
SN  - 18735061
KW  - Prostate stern cells
KW  - Epithelial-to-mesenchymal transition
KW  - Snai2/Slug
KW  - Organoids
KW  - Sternness
UR  - https://doi.org/10.1016/j.scr.2020.101844
L2  - https://doi.org/10.1016/j.scr.2020.101844
N2  - Deciphering the properties of adult stem cells is crucial for understanding of their role in healthy tissue and in cancer progression as well. Both stem cells and cancer stem cells have shown association with epithelial-to-mesenchymal transition (EMT) in various tissue types. Aiming to investigate the epithelial and mesenchymal phenotypic traits in adult mouse prostate, we sorted subpopulations of basal prostate stem cells (mPSCs) and assessed the expression levels of EMT regulators and markers with custom-designed gene expression array. The population of mPSCs defined by a Lin(-)/Sca-1(+) CD49f(hi)/Trop-2(+) (LSC Trop-(2+)) surface phenotype was enriched in mesenchymal markers, especially EMT master regulator Slug, encoded by the Snail gene. To further dissect the role of Slug in mPSCs, we used transgenic Snai2(tm1.1wbg) reporter mouse strain. Using this model, we confirmed the presence of mesenchymal traits and increase of organoid forming capacity in Slug(+) population of mPSCs. The Slug(+) -derived organoids comprised all prostate epithelial cell types - basal, luminal, and neuroendocrine. Collectively, these data uncover the important role of Slug expression in the physiology of mouse prostate stem cells.
ER  -

KAHOUNOVÁ, Zuzana, Ján REMŠÍK, Radek FEDR, Jan BOUCHAL, Alena MIČKOVÁ, Eva SLABÁKOVÁ, Lucia BINÓ, Aleš HAMPL a Karel SOUČEK. Slug-expressing mouse prostate epithelial cells have increased stem cell potential. \textit{Stem cell research}. Amsterdam: Elsevier, 2020, roč.~46, July 2020, s.~1-10. ISSN~1873-5061. Dostupné z: https://dx.doi.org/10.1016/j.scr.2020.101844.

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