Role of folding kinetics of secondary structures in telomeric
G-overhangs in the regulation of telomere maintenance
inSaccharomyces cerevisiae

J 2020

Role of folding kinetics of secondary structures in telomeric G-overhangs in the regulation of telomere maintenance inSaccharomyces cerevisiae

JURIKOVA, K., Martin GAJARSKÝ, M. HAJIKAZEMI, J. NOSEK, K. PROCHAZKOVA et. al.

Základní údaje

Originální název

Role of folding kinetics of secondary structures in telomeric G-overhangs in the regulation of telomere maintenance inSaccharomyces cerevisiae

Autoři

JURIKOVA, K., Martin GAJARSKÝ (703 Slovensko, domácí), M. HAJIKAZEMI, J. NOSEK, K. PROCHAZKOVA, K. PAESCHKE, Lukáš TRANTÍREK (203 Česká republika, garant, domácí) a L. TOMASKA

Vydání

Journal of Biological Chemistry, Bethesda, USA, Amer. Soc. Biochem. Mol. Biol. 2020, 0021-9258

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.157

Kód RIV

RIV/00216224:14740/20:00114648

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1074/jbc.RA120.012914

UT WoS

000550698000007

Klíčová slova anglicky

telomere; telomerase; Saccharomyces cerevisiae; cell cycle; Cdc13; G-hairpin; G-quadruplex; folding kinetics

Štítky

CF BIC, CF NMR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 10. 2024 15:51, Ing. Marie Švancarová

Anotace

V originále

The ends of eukaryotic chromosomes typically contain a 3? ssDNA G-rich protrusion (G-overhang). This overhang must be protected against detrimental activities of nucleases and of the DNA damage response machinery and participates in the regulation of telomerase, a ribonucleoprotein complex that maintains telomere integrity. These functions are mediated by DNA-binding proteins, such as Cdc13 inSaccharomyces cerevisiae, and the propensity of G-rich sequences to form various non-B DNA structures. Using CD and NMR spectroscopies, we show here that G-overhangs ofS. cerevisiaeform distinct Hoogsteen pairing?based secondary structures, depending on their length. Whereas short telomeric oligonucleotides form a G-hairpin, their longer counterparts form parallel and/or antiparallel G-quadruplexes (G4s). Regardless of their topologies, non-B DNA structures exhibited impaired binding to Cdc13in vitroas demonstrated by electrophoretic mobility shift assays. Importantly, whereas G4 structures formed relatively quickly, G-hairpins folded extremely slowly, indicating that short G-overhangs, which are typical for most of the cell cycle, are present predominantly as single-stranded oligonucleotides and are suitable substrates for Cdc13. Using ChIP, we show that the occurrence of G4 structures peaks at the late S phase, thus correlating with the accumulation of long G-overhangs. We present a model of how time- and length-dependent formation of non-B DNA structures at chromosomal termini participates in telomere maintenance.

Návaznosti

GA17-12075S, projekt VaV

Název: Polymorfní G-quadruplexy v promotorových oblastech genů

Investor: Grantová agentura ČR, Polymorfní G-quadruplexy v promotorových oblastech genů

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

NV19-08-00450, projekt VaV

Název: Atomárně rozlišená NMR spektroskopie in vivo jako nástroj pro biologické testování terapeuticky významných cílů v genomové ne-kanonické DNA a jejich interakcí s léčivy ve fenotypově diverzifikovaných nádorových buňkách.

Investor: Ministerstvo zdravotnictví ČR, Atomically resolved in vivo NMR spectroscopy as a novel tool for biological testing of therapeutically important genomic non-canonical DNA targets and their interactions with drugs in phenotypically diversified cancer cells.

90043, velká výzkumná infrastruktura

Název: CIISB

Citovat

JURIKOVA, K., Martin GAJARSKÝ, M. HAJIKAZEMI, J. NOSEK, K. PROCHAZKOVA, K. PAESCHKE, Lukáš TRANTÍREK a L. TOMASKA. Role of folding kinetics of secondary structures in telomeric G-overhangs in the regulation of telomere maintenance inSaccharomyces cerevisiae. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2020, roč. 295, č. 27, s. 8958-8971. ISSN 0021-9258. Dostupné z: https://dx.doi.org/10.1074/jbc.RA120.012914.

@article{1724876,
   author = {Jurikova, K. and Gajarský, Martin and Hajikazemi, M. and Nosek, J. and Prochazkova, K. and Paeschke, K. and Trantírek, Lukáš and Tomaska, L.},
   article_location = {Bethesda, USA},
   article_number = {27},
   doi = {http://dx.doi.org/10.1074/jbc.RA120.012914},
   keywords = {telomere; telomerase; Saccharomyces cerevisiae; cell cycle; Cdc13; G-hairpin; G-quadruplex; folding kinetics},
   language = {eng},
   issn = {0021-9258},
   journal = {Journal of Biological Chemistry},
   title = {Role of folding kinetics of secondary structures in telomeric G-overhangs in the regulation of telomere maintenance inSaccharomyces cerevisiae},
   url = {https://www.jbc.org/article/S0021-9258(17)50320-8/pdf},
   volume = {295},
   year = {2020}
}

TY  - JOUR
ID  - 1724876
AU  - Jurikova, K. - Gajarský, Martin - Hajikazemi, M. - Nosek, J. - Prochazkova, K. - Paeschke, K. - Trantírek, Lukáš - Tomaska, L.
PY  - 2020
TI  - Role of folding kinetics of secondary structures in telomeric G-overhangs in the regulation of telomere maintenance inSaccharomyces cerevisiae
JF  - Journal of Biological Chemistry
VL  - 295
IS  - 27
SP  - 8958-8971
EP  - 8958-8971
PB  - Amer. Soc. Biochem. Mol. Biol.
SN  - 00219258
KW  - telomere
KW  - telomerase
KW  - Saccharomyces cerevisiae
KW  - cell cycle
KW  - Cdc13
KW  - G-hairpin
KW  - G-quadruplex
KW  - folding kinetics
UR  - https://www.jbc.org/article/S0021-9258(17)50320-8/pdf
N2  - The ends of eukaryotic chromosomes typically contain a 3? ssDNA G-rich protrusion (G-overhang). This overhang must be protected against detrimental activities of nucleases and of the DNA damage response machinery and participates in the regulation of telomerase, a ribonucleoprotein complex that maintains telomere integrity. These functions are mediated by DNA-binding proteins, such as Cdc13 inSaccharomyces cerevisiae, and the propensity of G-rich sequences to form various non-B DNA structures. Using CD and NMR spectroscopies, we show here that G-overhangs ofS. cerevisiaeform distinct Hoogsteen pairing?based secondary structures, depending on their length. Whereas short telomeric oligonucleotides form a G-hairpin, their longer counterparts form parallel and/or antiparallel G-quadruplexes (G4s). Regardless of their topologies, non-B DNA structures exhibited impaired binding to Cdc13in vitroas demonstrated by electrophoretic mobility shift assays. Importantly, whereas G4 structures formed relatively quickly, G-hairpins folded extremely slowly, indicating that short G-overhangs, which are typical for most of the cell cycle, are present predominantly as single-stranded oligonucleotides and are suitable substrates for Cdc13. Using ChIP, we show that the occurrence of G4 structures peaks at the late S phase, thus correlating with the accumulation of long G-overhangs. We present a model of how time- and length-dependent formation of non-B DNA structures at chromosomal termini participates in telomere maintenance.
ER  -

JURIKOVA, K., Martin GAJARSKÝ, M. HAJIKAZEMI, J. NOSEK, K. PROCHAZKOVA, K. PAESCHKE, Lukáš TRANTÍREK a L. TOMASKA. Role of folding kinetics of secondary structures in telomeric G-overhangs in the regulation of telomere maintenance inSaccharomyces cerevisiae. \textit{Journal of Biological Chemistry}. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2020, roč.~295, č.~27, s.~8958-8971. ISSN~0021-9258. Dostupné z: https://dx.doi.org/10.1074/jbc.RA120.012914.

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