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@article{1724876, author = {Jurikova, K. and Gajarský, Martin and Hajikazemi, M. and Nosek, J. and Prochazkova, K. and Paeschke, K. and Trantírek, Lukáš and Tomaska, L.}, article_location = {Bethesda, USA}, article_number = {27}, doi = {http://dx.doi.org/10.1074/jbc.RA120.012914}, keywords = {telomere; telomerase; Saccharomyces cerevisiae; cell cycle; Cdc13; G-hairpin; G-quadruplex; folding kinetics}, language = {eng}, issn = {0021-9258}, journal = {Journal of Biological Chemistry}, title = {Role of folding kinetics of secondary structures in telomeric G-overhangs in the regulation of telomere maintenance inSaccharomyces cerevisiae}, url = {https://www.jbc.org/article/S0021-9258(17)50320-8/pdf}, volume = {295}, year = {2020} }
TY - JOUR ID - 1724876 AU - Jurikova, K. - Gajarský, Martin - Hajikazemi, M. - Nosek, J. - Prochazkova, K. - Paeschke, K. - Trantírek, Lukáš - Tomaska, L. PY - 2020 TI - Role of folding kinetics of secondary structures in telomeric G-overhangs in the regulation of telomere maintenance inSaccharomyces cerevisiae JF - Journal of Biological Chemistry VL - 295 IS - 27 SP - 8958-8971 EP - 8958-8971 PB - Amer. Soc. Biochem. Mol. Biol. SN - 00219258 KW - telomere KW - telomerase KW - Saccharomyces cerevisiae KW - cell cycle KW - Cdc13 KW - G-hairpin KW - G-quadruplex KW - folding kinetics UR - https://www.jbc.org/article/S0021-9258(17)50320-8/pdf N2 - The ends of eukaryotic chromosomes typically contain a 3? ssDNA G-rich protrusion (G-overhang). This overhang must be protected against detrimental activities of nucleases and of the DNA damage response machinery and participates in the regulation of telomerase, a ribonucleoprotein complex that maintains telomere integrity. These functions are mediated by DNA-binding proteins, such as Cdc13 inSaccharomyces cerevisiae, and the propensity of G-rich sequences to form various non-B DNA structures. Using CD and NMR spectroscopies, we show here that G-overhangs ofS. cerevisiaeform distinct Hoogsteen pairing?based secondary structures, depending on their length. Whereas short telomeric oligonucleotides form a G-hairpin, their longer counterparts form parallel and/or antiparallel G-quadruplexes (G4s). Regardless of their topologies, non-B DNA structures exhibited impaired binding to Cdc13in vitroas demonstrated by electrophoretic mobility shift assays. Importantly, whereas G4 structures formed relatively quickly, G-hairpins folded extremely slowly, indicating that short G-overhangs, which are typical for most of the cell cycle, are present predominantly as single-stranded oligonucleotides and are suitable substrates for Cdc13. Using ChIP, we show that the occurrence of G4 structures peaks at the late S phase, thus correlating with the accumulation of long G-overhangs. We present a model of how time- and length-dependent formation of non-B DNA structures at chromosomal termini participates in telomere maintenance. ER -
JURIKOVA, K., Martin GAJARSKÝ, M. HAJIKAZEMI, J. NOSEK, K. PROCHAZKOVA, K. PAESCHKE, Lukáš TRANTÍREK a L. TOMASKA. Role of folding kinetics of secondary structures in telomeric G-overhangs in the regulation of telomere maintenance inSaccharomyces cerevisiae. \textit{Journal of Biological Chemistry}. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2020, roč.~295, č.~27, s.~8958-8971. ISSN~0021-9258. Dostupné z: https://dx.doi.org/10.1074/jbc.RA120.012914.
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