BROFT, P., Šimon DŽATKO, Michaela KRAFČÍKOVÁ, A. WACKER, Robert HANSEL-HERTSCH, Volker DOTSCH, Lukáš TRANTÍREK a Harald SCHWALBE. In-Cell NMR Spectroscopy of Functional Riboswitch Aptamers in Eukaryotic Cells. Angewandte Chemie International Edition. Weinheim: Wiley-VCH Verlag, 2021, roč. 60, č. 2, s. 865-872, 10 s. ISSN 1433-7851. Dostupné z: https://dx.doi.org/10.1002/anie.202007184. |
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@article{1726480, author = {Broft, P. and Džatko, Šimon and Krafčíková, Michaela and Wacker, A. and HanselandHertsch, Robert and Dotsch, Volker and Trantírek, Lukáš and Schwalbe, Harald}, article_location = {Weinheim}, article_number = {2}, doi = {http://dx.doi.org/10.1002/anie.202007184}, keywords = {aptamers; 2' -deoxyguanosine riboswitch; HeLa cells; RNA structures; structural biology}, language = {eng}, issn = {1433-7851}, journal = {Angewandte Chemie International Edition}, title = {In-Cell NMR Spectroscopy of Functional Riboswitch Aptamers in Eukaryotic Cells}, url = {https://doi.org/10.1002/anie.202007184}, volume = {60}, year = {2021} }
TY - JOUR ID - 1726480 AU - Broft, P. - Džatko, Šimon - Krafčíková, Michaela - Wacker, A. - Hansel-Hertsch, Robert - Dotsch, Volker - Trantírek, Lukáš - Schwalbe, Harald PY - 2021 TI - In-Cell NMR Spectroscopy of Functional Riboswitch Aptamers in Eukaryotic Cells JF - Angewandte Chemie International Edition VL - 60 IS - 2 SP - 865-872 EP - 865-872 PB - Wiley-VCH Verlag SN - 14337851 KW - aptamers KW - 2' -deoxyguanosine riboswitch KW - HeLa cells KW - RNA structures KW - structural biology UR - https://doi.org/10.1002/anie.202007184 L2 - https://doi.org/10.1002/anie.202007184 N2 - We report here the in-cell NMR-spectroscopic observation of the binding of the cognate ligand 2 '-deoxyguanosine to the aptamer domain of the bacterial 2 '-deoxyguanosine-sensing riboswitch in eukaryotic cells, namely Xenopus laevis oocytes and in human HeLa cells. The riboswitch is sufficiently stable in both cell types to allow for detection of binding of the ligand to the riboswitch. Most importantly, we show that the binding mode established by in vitro characterization of this prokaryotic riboswitch is maintained in eukaryotic cellular environment. Our data also bring important methodological insights: Thus far, in-cell NMR studies on RNA in mammalian cells have been limited to investigations of short (<15 nt) RNA fragments that were extensively modified by protecting groups to limit their degradation in the intracellular space. Here, we show that the in-cell NMR setup can be adjusted for characterization of much larger (approximate to 70 nt) functional and chemically non-modified RNA. ER -
BROFT, P., Šimon DŽATKO, Michaela KRAFČÍKOVÁ, A. WACKER, Robert HANSEL-HERTSCH, Volker DOTSCH, Lukáš TRANTÍREK a Harald SCHWALBE. In-Cell NMR Spectroscopy of Functional Riboswitch Aptamers in Eukaryotic Cells. \textit{Angewandte Chemie International Edition}. Weinheim: Wiley-VCH Verlag, 2021, roč.~60, č.~2, s.~865-872, 10 s. ISSN~1433-7851. Dostupné z: https://dx.doi.org/10.1002/anie.202007184.
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