Rapid HPLC method for monitoring of lactulose production with a high yield

PAZOUREK, Jiří. Rapid HPLC method for monitoring of lactulose production with a high yield. Carbohydrate Research. Elsevier, 2019, vol. 484, No 107773, p. 1-5. ISSN 0008-6215. Available from: https://dx.doi.org/10.1016/j.carres.2019.107773.

Basic information

• Original name: Rapid HPLC method for monitoring of lactulose production with a high yield
• Authors: PAZOUREK, Jiří (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Carbohydrate Research, Elsevier, 2019, 0008-6215.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 21101 Food and beverages
• Country of publisher: Netherlands
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 1.841
• RIV identification code: RIV/00216224:14160/19:00120952
• Organization unit: Faculty of Pharmacy
• Doi: http://dx.doi.org/10.1016/j.carres.2019.107773
• UT WoS: 000487202700004
• Keywords (in Czech): Laktosa; výroba laktulosy; monitoring; hydrofilní interakční kapalinová chromatografie; stanovení
• Keywords in English: Lactose; Lactulose production; Monitoring; Hydrophilic interaction liquid chromatography; Determination
• Tags: Reviewed

Abstract

An HPLC method suitable for rapid monitoring of lactulose production by isomerization from lactose was developed. The separation of lactose and lactulose under hydrophilic interaction liquid chromatography (HILIC) mode was achieved with resolution 1.5 within 5 min. Since isocratic elution was used, there is no extra time necessary for the column equilibration. Application of the method was illustrated on monitoring lactulose isomerization with catalysis of sodium hydroxide in the presence of sodium tetraborate at 70 °C (pH=11). The conversion yield obtained for lactulose was 86%, and corresponding purity 76%. For the first time, a polyhydroxy stationary phase for separation of lactose and lactulose is reported.