Behaviour of Vascular Smooth Muscle Cells on Amine
Plasma-Coated Materials with Various Chemical Structures and
Morphologies

J 2020

Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies

NEMCAKOVA, Ivana, Lucie BLAHOVÁ, Petr RYSANEK, Andreu BLANQUER, Lucie BACAKOVA et. al.

Basic information

Original name

Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies

Authors

NEMCAKOVA, Ivana, Lucie BLAHOVÁ (203 Czech Republic, belonging to the institution), Petr RYSANEK, Andreu BLANQUER, Lucie BACAKOVA and Lenka ZAJÍČKOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

International Journal of Molecular Sciences, Basel, MDPI, 2020, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10305 Fluids and plasma physics

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.923

RIV identification code

RIV/00216224:14310/20:00114673

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/ijms21249467

UT WoS

000602830000001

Keywords in English

amine plasma polymer; bioactive coating; polycaprolactone nanofibers; substrate morphology; cell adhesion; cell proliferation

Abstract

V originále

Amine-coated biodegradable materials based on synthetic polymers have a great potential for tissue remodeling and regeneration because of their excellent processability and bioactivity. In the present study, we have investigated the influence of various chemical compositions of amine plasma polymer (PP) coatings and the influence of the substrate morphology, represented by polystyrene culture dishes and polycaprolactone nanofibers (PCL NFs), on the behavior of vascular smooth muscle cells (VSMCs). Although all amine-PP coatings improved the initial adhesion of VSMCs, 7-day long cultivation revealed a clear preference for the coating containing about 15 at.% of nitrogen (CPA-33). The CPA-33 coating demonstrated the ideal combination of good water stability, a sufficient amine group content, and favorable surface wettability and morphology. The nanostructured morphology of amine-PP-coated PCL NFs successfully slowed the proliferation rate of VSMCs, which is essential in preventing restenosis of vascular replacements in vivo. At the same time, CPA-33-coated PCL NFs supported the continuous proliferation of VSMCs during 7-day long cultivation, with no significant increase in cytokine secretion by RAW 264.7 macrophages. The CPA-33 coating deposited on biodegradable PCL NFs therefore seems to be a promising material for manufacturing small-diameter vascular grafts, which are still lacking on the current market.

Links

GA18-12774S, research and development project

Name: Plazmové polymery připravené na nanovlákenných membránách pro inženýrství cévní tkáně

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

90110, large research infrastructures

Name: CzechNanoLab

Citovat

NEMCAKOVA, Ivana, Lucie BLAHOVÁ, Petr RYSANEK, Andreu BLANQUER, Lucie BACAKOVA and Lenka ZAJÍČKOVÁ. Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies. International Journal of Molecular Sciences. Basel: MDPI, 2020, vol. 21, No 24, p. 1-24. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms21249467.

@article{1728079,
   author = {Nemcakova, Ivana and Blahová, Lucie and Rysanek, Petr and Blanquer, Andreu and Bacakova, Lucie and Zajíčková, Lenka},
   article_location = {Basel},
   article_number = {24},
   doi = {http://dx.doi.org/10.3390/ijms21249467},
   keywords = {amine plasma polymer; bioactive coating; polycaprolactone nanofibers; substrate morphology; cell adhesion; cell proliferation},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   note = {doc. Zajíčková - dle rozhodnutí ředitelů 5.3.2024},
   title = {Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies},
   url = {https://doi.org/10.3390/ijms21249467},
   volume = {21},
   year = {2020}
}

TY  - JOUR
ID  - 1728079
AU  - Nemcakova, Ivana - Blahová, Lucie - Rysanek, Petr - Blanquer, Andreu - Bacakova, Lucie - Zajíčková, Lenka
PY  - 2020
TI  - Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies
JF  - International Journal of Molecular Sciences
VL  - 21
IS  - 24
SP  - 1-24
EP  - 1-24
PB  - MDPI
SN  - 14220067
N1  - doc. Zajíčková - dle rozhodnutí ředitelů 5.3.2024
KW  - amine plasma polymer
KW  - bioactive coating
KW  - polycaprolactone nanofibers
KW  - substrate morphology
KW  - cell adhesion
KW  - cell proliferation
UR  - https://doi.org/10.3390/ijms21249467
L2  - https://doi.org/10.3390/ijms21249467
N2  - Amine-coated biodegradable materials based on synthetic polymers have a great potential for tissue remodeling and regeneration because of their excellent processability and bioactivity. In the present study, we have investigated the influence of various chemical compositions of amine plasma polymer (PP) coatings and the influence of the substrate morphology, represented by polystyrene culture dishes and polycaprolactone nanofibers (PCL NFs), on the behavior of vascular smooth muscle cells (VSMCs). Although all amine-PP coatings improved the initial adhesion of VSMCs, 7-day long cultivation revealed a clear preference for the coating containing about 15 at.% of nitrogen (CPA-33). The CPA-33 coating demonstrated the ideal combination of good water stability, a sufficient amine group content, and favorable surface wettability and morphology. The nanostructured morphology of amine-PP-coated PCL NFs successfully slowed the proliferation rate of VSMCs, which is essential in preventing restenosis of vascular replacements in vivo. At the same time, CPA-33-coated PCL NFs supported the continuous proliferation of VSMCs during 7-day long cultivation, with no significant increase in cytokine secretion by RAW 264.7 macrophages. The CPA-33 coating deposited on biodegradable PCL NFs therefore seems to be a promising material for manufacturing small-diameter vascular grafts, which are still lacking on the current market.
ER  -

NEMCAKOVA, Ivana, Lucie BLAHOVÁ, Petr RYSANEK, Andreu BLANQUER, Lucie BACAKOVA and Lenka ZAJÍČKOVÁ. Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies. \textit{International Journal of Molecular Sciences}. Basel: MDPI, 2020, vol.~21, No~24, p.~1-24. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms21249467.

Detailed Information on Publication Record