A prolonged exposure of human lung carcinoma epithelial cells
to benzo[a]pyrene induces p21-dependent
epithelial-to-mesenchymal transition (EMT)-like phenotype

J 2021

A prolonged exposure of human lung carcinoma epithelial cells to benzo[a]pyrene induces p21-dependent epithelial-to-mesenchymal transition (EMT)-like phenotype

HYZDALOVA, Martina, Jiřina PROCHÁZKOVÁ, Simona STRAPACOVA, Lucie SVRZKOVA, Ondřej VACEK et. al.

Základní údaje

Originální název

A prolonged exposure of human lung carcinoma epithelial cells to benzo[a]pyrene induces p21-dependent epithelial-to-mesenchymal transition (EMT)-like phenotype

Autoři

HYZDALOVA, Martina, Jiřina PROCHÁZKOVÁ (203 Česká republika), Simona STRAPACOVA, Lucie SVRZKOVA, Ondřej VACEK (203 Česká republika, domácí), Radek FEDR, Zdeněk ANDRYSÍK (203 Česká republika), Eva HRUBA, Helena LIBALOVA, Jiri KLEMA, Jan TOPINKA, Josef MASEK, Karel SOUČEK (203 Česká republika), Jan VONDRÁČEK (203 Česká republika) a Miroslav MACHALA (203 Česká republika, garant)

Vydání

Chemosphere, Oxford, Pergamon-Elsevier Science Ltd, 2021, 0045-6535

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30108 Toxicology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 8.943

Kód RIV

RIV/00216224:14310/21:00120970

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.chemosphere.2020.128126

UT WoS

000595802200223

Klíčová slova anglicky

BaP; TCDD; Lung carcinoma; Cell proliferation; EMT; Tumor progression

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 5. 2024 08:11, Mgr. Marie Šípková, DiS.

Anotace

V originále

Deciphering the role of the aryl hydrocarbon receptor (AhR) in lung cancer cells may help us to better understand the role of toxic AhR ligands in lung carcinogenesis, including cancer progression. We employed human lung carcinoma A549 cells to investigate their fate after continuous two-week exposure to model AhR agonists, genotoxic benzo[a]pyrene (BaP; 1 mu M) and non-genotoxic 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; 10 nM). While TCDD increased proliferative rate of A549 cells, exposure to BaP decreased cell proliferation and induced epithelial-to-mesenchymal transition (EMT)-like phenotype, which was associated with enhanced cell migration, invasion, and altered cell morphology. Although TCDD also suppressed expression of E-cadherin and activated some genes linked to EMT, it did not induce the EMT-like phenotype. The results of transcriptomic analysis, and the opposite effects of BaP and TCDD on cell proliferation, indicated that a delay in cell cycle progression, together with a slight increase of senescence (when coupled with AhR activation), favors the induction of EMT-like phenotype. The shift towards EMT-like phenotype observed after simultaneous treatment with TCDD and mitomycin C (an inhibitor of cell proliferation) confirmed the hypothesis. Since BaP decreased cell proliferative rate via induction of p21 expression, we generated the A549 cell model with reduced p21 expression and exposed it to BaP for two weeks. The p21 knockdown suppressed the BaP-mediated EMT-like phenotype in A549 cells, thus confirming that a delayed cell cycle progression, together with p21-dependent induction of senescence-related chemokine CCL2, may contribute to induction of EMT-like cell phenotype in lung cells exposed to genotoxic AhR ligands.

Citovat

HYZDALOVA, Martina, Jiřina PROCHÁZKOVÁ, Simona STRAPACOVA, Lucie SVRZKOVA, Ondřej VACEK, Radek FEDR, Zdeněk ANDRYSÍK, Eva HRUBA, Helena LIBALOVA, Jiri KLEMA, Jan TOPINKA, Josef MASEK, Karel SOUČEK, Jan VONDRÁČEK a Miroslav MACHALA. A prolonged exposure of human lung carcinoma epithelial cells to benzo[a]pyrene induces p21-dependent epithelial-to-mesenchymal transition (EMT)-like phenotype. Chemosphere. Oxford: Pergamon-Elsevier Science Ltd, 2021, roč. 263, January 2021, s. 1-15. ISSN 0045-6535. Dostupné z: https://dx.doi.org/10.1016/j.chemosphere.2020.128126.

@article{1728092,
   author = {Hyzdalova, Martina and Procházková, Jiřina and Strapacova, Simona and Svrzkova, Lucie and Vacek, Ondřej and Fedr, Radek and Andrysík, Zdeněk and Hruba, Eva and Libalova, Helena and Klema, Jiri and Topinka, Jan and Masek, Josef and Souček, Karel and Vondráček, Jan and Machala, Miroslav},
   article_location = {Oxford},
   article_number = {January 2021},
   doi = {http://dx.doi.org/10.1016/j.chemosphere.2020.128126},
   keywords = {BaP; TCDD; Lung carcinoma; Cell proliferation; EMT; Tumor progression},
   language = {eng},
   issn = {0045-6535},
   journal = {Chemosphere},
   title = {A prolonged exposure of human lung carcinoma epithelial cells to benzo[a]pyrene induces p21-dependent epithelial-to-mesenchymal transition (EMT)-like phenotype},
   url = {https://doi.org/10.1016/j.chemosphere.2020.128126},
   volume = {263},
   year = {2021}
}

TY  - JOUR
ID  - 1728092
AU  - Hyzdalova, Martina - Procházková, Jiřina - Strapacova, Simona - Svrzkova, Lucie - Vacek, Ondřej - Fedr, Radek - Andrysík, Zdeněk - Hruba, Eva - Libalova, Helena - Klema, Jiri - Topinka, Jan - Masek, Josef - Souček, Karel - Vondráček, Jan - Machala, Miroslav
PY  - 2021
TI  - A prolonged exposure of human lung carcinoma epithelial cells to benzo[a]pyrene induces p21-dependent epithelial-to-mesenchymal transition (EMT)-like phenotype
JF  - Chemosphere
VL  - 263
IS  - January 2021
SP  - 1-15
EP  - 1-15
PB  - Pergamon-Elsevier Science Ltd
SN  - 00456535
KW  - BaP
KW  - TCDD
KW  - Lung carcinoma
KW  - Cell proliferation
KW  - EMT
KW  - Tumor progression
UR  - https://doi.org/10.1016/j.chemosphere.2020.128126
L2  - https://doi.org/10.1016/j.chemosphere.2020.128126
N2  - Deciphering the role of the aryl hydrocarbon receptor (AhR) in lung cancer cells may help us to better understand the role of toxic AhR ligands in lung carcinogenesis, including cancer progression. We employed human lung carcinoma A549 cells to investigate their fate after continuous two-week exposure to model AhR agonists, genotoxic benzo[a]pyrene (BaP; 1 mu M) and non-genotoxic 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; 10 nM). While TCDD increased proliferative rate of A549 cells, exposure to BaP decreased cell proliferation and induced epithelial-to-mesenchymal transition (EMT)-like phenotype, which was associated with enhanced cell migration, invasion, and altered cell morphology. Although TCDD also suppressed expression of E-cadherin and activated some genes linked to EMT, it did not induce the EMT-like phenotype. The results of transcriptomic analysis, and the opposite effects of BaP and TCDD on cell proliferation, indicated that a delay in cell cycle progression, together with a slight increase of senescence (when coupled with AhR activation), favors the induction of EMT-like phenotype. The shift towards EMT-like phenotype observed after simultaneous treatment with TCDD and mitomycin C (an inhibitor of cell proliferation) confirmed the hypothesis. Since BaP decreased cell proliferative rate via induction of p21 expression, we generated the A549 cell model with reduced p21 expression and exposed it to BaP for two weeks. The p21 knockdown suppressed the BaP-mediated EMT-like phenotype in A549 cells, thus confirming that a delayed cell cycle progression, together with p21-dependent induction of senescence-related chemokine CCL2, may contribute to induction of EMT-like cell phenotype in lung cells exposed to genotoxic AhR ligands.
ER  -

HYZDALOVA, Martina, Jiřina PROCHÁZKOVÁ, Simona STRAPACOVA, Lucie SVRZKOVA, Ondřej VACEK, Radek FEDR, Zdeněk ANDRYSÍK, Eva HRUBA, Helena LIBALOVA, Jiri KLEMA, Jan TOPINKA, Josef MASEK, Karel SOUČEK, Jan VONDRÁČEK a Miroslav MACHALA. A prolonged exposure of human lung carcinoma epithelial cells to benzo[a]pyrene induces p21-dependent epithelial-to-mesenchymal transition (EMT)-like phenotype. \textit{Chemosphere}. Oxford: Pergamon-Elsevier Science Ltd, 2021, roč.~263, January 2021, s.~1-15. ISSN~0045-6535. Dostupné z: https://dx.doi.org/10.1016/j.chemosphere.2020.128126.

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