A novel accurate LC-MS/MS method for quantitative determination
of Z-lumirubin

J 2020

A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin

JASPROVA, Jana, Ales DVORAK, Marek VECKA, Martin LENICEK, Ondrej LACINA et. al.

Základní údaje

Originální název

A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin

Autoři

JASPROVA, Jana (203 Česká republika), Ales DVORAK (203 Česká republika), Marek VECKA (203 Česká republika), Martin LENICEK (203 Česká republika), Ondrej LACINA (203 Česká republika), Petra VALASKOVA (203 Česká republika), Milos ZAPADLO (203 Česká republika), Richard PLAVKA (203 Česká republika), Petr KLÁN (203 Česká republika, garant, domácí) a Libor VITEK (203 Česká republika)

Vydání

Scientific reports, LONDON, NATURE PUBLISHING GROUP, 2020, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10700 1.7 Other natural sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.379

Kód RIV

RIV/00216224:14310/20:00117827

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1038/s41598-020-61280-z

UT WoS

000563374600009

Klíčová slova anglicky

bilirubin; lumirubin; liquid chromatography; clinical use

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 2. 2023 21:19, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Although phototherapy (PT) is a standard treatment for neonatal jaundice, no validated clinical methods for determination of bilirubin phototherapy products are available. Thus, the aim of our study was to establish a such method for clinical use. To achieve this aim, a LC-MS/MS assay for simultaneous determination of Z-lumirubin (LR) and unconjugated bilirubin (UCB) was conducted. LR was purified after irradiation of UCB at 460 nm. The assay was tested on human sera from PT-treated neonates. Samples were separated on a HPLC system with a triple quadrupole mass spectrometer detector. The instrument response was linear up to 5.8 and 23.4 mg/dL for LR and UCB, respectively, with submicromolar limits of detection and validity parameters relevant for use in clinical medicine. Exposure of newborns to PT raised serum LR concentrations three-fold (p < 0.01), but the absolute concentrations were low (0.37 +/- 0.16 mg/dL), despite a dramatic decrease of serum UCB concentrations (13.6 +/- 2.2 vs. 10.3 +/- 3.3 mg/dL, p < 0.01). A LC-MS/MS method for the simultaneous determination of LR and UCB in human serum was established and validated for clinical use. This method should help to monitor neonates on PT, as well as to improve our understanding of both the kinetics and biology of bilirubin phototherapy products.

Návaznosti

NV18-07-00342, projekt VaV

Název: Studium degradačních produktů bilirubinu vznikajících při fototerapii novorozenecké žloutenky

Investor: Ministerstvo zdravotnictví ČR, Studium degradačních produktů bilirubinu vznikajících při fototerapii novorozenecké žloutenky

Citovat

JASPROVA, Jana, Ales DVORAK, Marek VECKA, Martin LENICEK, Ondrej LACINA, Petra VALASKOVA, Milos ZAPADLO, Richard PLAVKA, Petr KLÁN a Libor VITEK. A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin. Scientific reports. LONDON: NATURE PUBLISHING GROUP, 2020, roč. 10, č. 1, s. 1-8. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-020-61280-z.

@article{1728976,
   author = {Jasprova, Jana and Dvorak, Ales and Vecka, Marek and Lenicek, Martin and Lacina, Ondrej and Valaskova, Petra and Zapadlo, Milos and Plavka, Richard and Klán, Petr and Vitek, Libor},
   article_location = {LONDON},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-020-61280-z},
   keywords = {bilirubin; lumirubin; liquid chromatography; clinical use},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific reports},
   title = {A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin},
   url = {https://www.nature.com/articles/s41598-020-61280-z},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 1728976
AU  - Jasprova, Jana - Dvorak, Ales - Vecka, Marek - Lenicek, Martin - Lacina, Ondrej - Valaskova, Petra - Zapadlo, Milos - Plavka, Richard - Klán, Petr - Vitek, Libor
PY  - 2020
TI  - A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin
JF  - Scientific reports
VL  - 10
IS  - 1
SP  - 1-8
EP  - 1-8
PB  - NATURE PUBLISHING GROUP
SN  - 20452322
KW  - bilirubin
KW  - lumirubin
KW  - liquid chromatography
KW  - clinical use
UR  - https://www.nature.com/articles/s41598-020-61280-z
L2  - https://www.nature.com/articles/s41598-020-61280-z
N2  - Although phototherapy (PT) is a standard treatment for neonatal jaundice, no validated clinical methods for determination of bilirubin phototherapy products are available. Thus, the aim of our study was to establish a such method for clinical use. To achieve this aim, a LC-MS/MS assay for simultaneous determination of Z-lumirubin (LR) and unconjugated bilirubin (UCB) was conducted. LR was purified after irradiation of UCB at 460 nm. The assay was tested on human sera from PT-treated neonates. Samples were separated on a HPLC system with a triple quadrupole mass spectrometer detector. The instrument response was linear up to 5.8 and 23.4 mg/dL for LR and UCB, respectively, with submicromolar limits of detection and validity parameters relevant for use in clinical medicine. Exposure of newborns to PT raised serum LR concentrations three-fold (p < 0.01), but the absolute concentrations were low (0.37 +/- 0.16 mg/dL), despite a dramatic decrease of serum UCB concentrations (13.6 +/- 2.2 vs. 10.3 +/- 3.3 mg/dL, p < 0.01). A LC-MS/MS method for the simultaneous determination of LR and UCB in human serum was established and validated for clinical use. This method should help to monitor neonates on PT, as well as to improve our understanding of both the kinetics and biology of bilirubin phototherapy products.
ER  -

JASPROVA, Jana, Ales DVORAK, Marek VECKA, Martin LENICEK, Ondrej LACINA, Petra VALASKOVA, Milos ZAPADLO, Richard PLAVKA, Petr KLÁN a Libor VITEK. A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin. \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, 2020, roč.~10, č.~1, s.~1-8. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-020-61280-z.

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