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@article{1728976,
author = {Jasprova, Jana and Dvorak, Ales and Vecka, Marek and Lenicek, Martin and Lacina, Ondrej and Valaskova, Petra and Zapadlo, Milos and Plavka, Richard and Klán, Petr and Vitek, Libor},
article_location = {LONDON},
article_number = {1},
doi = {http://dx.doi.org/10.1038/s41598-020-61280-z},
keywords = {bilirubin; lumirubin; liquid chromatography; clinical use},
language = {eng},
issn = {2045-2322},
journal = {Scientific reports},
title = {A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin},
url = {https://www.nature.com/articles/s41598-020-61280-z},
volume = {10},
year = {2020}
}
TY - JOUR
ID - 1728976
AU - Jasprova, Jana - Dvorak, Ales - Vecka, Marek - Lenicek, Martin - Lacina, Ondrej - Valaskova, Petra - Zapadlo, Milos - Plavka, Richard - Klán, Petr - Vitek, Libor
PY - 2020
TI - A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin
JF - Scientific reports
VL - 10
IS - 1
SP - 1-8
EP - 1-8
PB - NATURE PUBLISHING GROUP
SN - 20452322
KW - bilirubin
KW - lumirubin
KW - liquid chromatography
KW - clinical use
UR - https://www.nature.com/articles/s41598-020-61280-z
L2 - https://www.nature.com/articles/s41598-020-61280-z
N2 - Although phototherapy (PT) is a standard treatment for neonatal jaundice, no validated clinical methods for determination of bilirubin phototherapy products are available. Thus, the aim of our study was to establish a such method for clinical use. To achieve this aim, a LC-MS/MS assay for simultaneous determination of Z-lumirubin (LR) and unconjugated bilirubin (UCB) was conducted. LR was purified after irradiation of UCB at 460 nm. The assay was tested on human sera from PT-treated neonates. Samples were separated on a HPLC system with a triple quadrupole mass spectrometer detector. The instrument response was linear up to 5.8 and 23.4 mg/dL for LR and UCB, respectively, with submicromolar limits of detection and validity parameters relevant for use in clinical medicine. Exposure of newborns to PT raised serum LR concentrations three-fold (p < 0.01), but the absolute concentrations were low (0.37 +/- 0.16 mg/dL), despite a dramatic decrease of serum UCB concentrations (13.6 +/- 2.2 vs. 10.3 +/- 3.3 mg/dL, p < 0.01). A LC-MS/MS method for the simultaneous determination of LR and UCB in human serum was established and validated for clinical use. This method should help to monitor neonates on PT, as well as to improve our understanding of both the kinetics and biology of bilirubin phototherapy products.
ER -
JASPROVA, Jana, Ales DVORAK, Marek VECKA, Martin LENICEK, Ondrej LACINA, Petra VALASKOVA, Milos ZAPADLO, Richard PLAVKA, Petr KLÁN a Libor VITEK. A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin. \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, 2020, roč.~10, č.~1, s.~1-8. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-020-61280-z.
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