LA-ICP-MS as a sensitive method for detection of
nanoparticle-antibody conjugates in immunochemistry analysis

D 2019

LA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysis

VLČNOVSKÁ, Marcela, Michaela KUCHYNKA, Aneta ŠTOSSOVÁ, Hana HOLCOVÁ POLANSKÁ, Marketa VACULOVICOVA et. al.

Základní údaje

Originální název

LA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysis

Autoři

VLČNOVSKÁ, Marcela (203 Česká republika, domácí), Michaela KUCHYNKA (203 Česká republika, domácí), Aneta ŠTOSSOVÁ (203 Česká republika, domácí), Hana HOLCOVÁ POLANSKÁ (203 Česká republika, domácí), Marketa VACULOVICOVA, Tomáš VACULOVIČ (203 Česká republika, domácí) a Michal MASAŘÍK (203 Česká republika, domácí)

Vydání

Brno, MENDELNET 2019: PROCEEDINGS OF 26TH INTERNATIONAL PHD STUDENTS CONFERENCE, od s. 682-686, 5 s. 2019

Nakladatel

MENDEL UNIV BRNO

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Odkazy

URL

Kód RIV

RIV/00216224:14310/19:00114693

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-7509-688-3

UT WoS

000576735500123

Klíčová slova anglicky

gold nanoparticles; LA-ICP-MS; immunochemistry; dot-blot; protein detection; p53

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 1. 2021 13:23, Mgr. Marie Šípková, DiS.

Anotace

V originále

Laser ablation followed by inductively coupled plasma mass spectrometry is an analytical method suitable for low concentration measurement of elements in a sample. This can be used not only for detection of elemental distribution within a sample but also as a detection means for immunoanalytical techniques using nanoparticle-antibody conjugates as recognition elements. The aim of this work was to prepare a conjugate of 10nm gold nanoparticles with anti-p53 antibody, to verify the antigen binding ability, and to use this construct for identification of p53 in a real sample of cell lysate. It has been experimentally verified that it is possible to use nanoparticle-modified antibodies for immunochemical analysis coupled to LA-ICP-MS detection. However, the nonspecific sorption of nanoparticles on the sample surface needs to be minimized by optimization of the blocking step.

Návaznosti

GA17-12774S, projekt VaV

Název: Využití vícenásobného značení pomocí kovových nanočástic pro bio-zobrazování

Investor: Grantová agentura ČR, Využití vícenásobného značení pomocí kovových nanočástic pro bio-zobrazování

MUNI/C/0003/2019, interní kód MU

Název: Multimodální značení protilátek pro specifické rozpoznávání vybraných antigenů

Investor: Masarykova univerzita, Multimodální značení protilátek pro specifické rozpoznávání vybraných antigenů, DO R. 2020 - Program rektora

Citovat

VLČNOVSKÁ, Marcela, Michaela KUCHYNKA, Aneta ŠTOSSOVÁ, Hana HOLCOVÁ POLANSKÁ, Marketa VACULOVICOVA, Tomáš VACULOVIČ a Michal MASAŘÍK. LA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysis. Online. In Cerkal, R Belcredi, NB Prokesova, L Pilatova, A. MENDELNET 2019: PROCEEDINGS OF 26TH INTERNATIONAL PHD STUDENTS CONFERENCE. Brno: MENDEL UNIV BRNO, 2019, s. 682-686. ISBN 978-80-7509-688-3.

@inproceedings{1729998,
   author = {Vlčnovská, Marcela and Kuchynka, Michaela and Štossová, Aneta and Holcová Polanská, Hana and Vaculovicova, Marketa and Vaculovič, Tomáš and Masařík, Michal},
   address = {Brno},
   booktitle = {MENDELNET 2019: PROCEEDINGS OF 26TH INTERNATIONAL PHD STUDENTS CONFERENCE},
   editor = {Cerkal, R Belcredi, NB Prokesova, L Pilatova, A},
   keywords = {gold nanoparticles; LA-ICP-MS; immunochemistry; dot-blot; protein detection; p53},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Brno},
   isbn = {978-80-7509-688-3},
   pages = {682-686},
   publisher = {MENDEL UNIV BRNO},
   title = {LA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysis},
   url = {https://mendelnet.cz/artkey/mnt-201901-0131_LA-ICP-MS-as-a-sensitive-method-for-detection-of-nanoparticle-antibody-conjugates-in-immunochemistry-analysis.php?back=/magno/mnt/2019/mn1.php?secid=4},
   year = {2019}
}

TY  - JOUR
ID  - 1729998
AU  - Vlčnovská, Marcela - Kuchynka, Michaela - Štossová, Aneta - Holcová Polanská, Hana - Vaculovicova, Marketa - Vaculovič, Tomáš - Masařík, Michal
PY  - 2019
TI  - LA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysis
PB  - MENDEL UNIV BRNO
CY  - Brno
SN  - 9788075096883
KW  - gold nanoparticles
KW  - LA-ICP-MS
KW  - immunochemistry
KW  - dot-blot
KW  - protein detection
KW  - p53
UR  - https://mendelnet.cz/artkey/mnt-201901-0131_LA-ICP-MS-as-a-sensitive-method-for-detection-of-nanoparticle-antibody-conjugates-in-immunochemistry-analysis.php?back=/magno/mnt/2019/mn1.php?secid=4
L2  - https://mendelnet.cz/artkey/mnt-201901-0131_LA-ICP-MS-as-a-sensitive-method-for-detection-of-nanoparticle-antibody-conjugates-in-immunochemistry-analysis.php?back=/magno/mnt/2019/mn1.php?secid=4
N2  - Laser ablation followed by inductively coupled plasma mass spectrometry is an analytical method suitable for low concentration measurement of elements in a sample. This can be used not only for detection of elemental distribution within a sample but also as a detection means for immunoanalytical techniques using nanoparticle-antibody conjugates as recognition elements. The aim of this work was to prepare a conjugate of 10nm gold nanoparticles with anti-p53 antibody, to verify the antigen binding ability, and to use this construct for identification of p53 in a real sample of cell lysate. It has been experimentally verified that it is possible to use nanoparticle-modified antibodies for immunochemical analysis coupled to LA-ICP-MS detection. However, the nonspecific sorption of nanoparticles on the sample surface needs to be minimized by optimization of the blocking step.
ER  -

VLČNOVSKÁ, Marcela, Michaela KUCHYNKA, Aneta ŠTOSSOVÁ, Hana HOLCOVÁ POLANSKÁ, Marketa VACULOVICOVA, Tomáš VACULOVIČ a Michal MASAŘÍK. LA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysis. Online. In Cerkal, R Belcredi, NB Prokesova, L Pilatova, A. \textit{MENDELNET 2019: PROCEEDINGS OF 26TH INTERNATIONAL PHD STUDENTS CONFERENCE}. Brno: MENDEL UNIV BRNO, 2019, s.~682-686. ISBN~978-80-7509-688-3.

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