| VLČNOVSKÁ, Marcela, Michaela KUCHYNKA, Aneta ŠTOSSOVÁ, Hana HOLCOVÁ POLANSKÁ, Marketa VACULOVICOVA, Tomáš VACULOVIČ and Michal MASAŘÍK. LA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysis. Online. In Cerkal, R Belcredi, NB Prokesova, L Pilatova, A. MENDELNET 2019: PROCEEDINGS OF 26TH INTERNATIONAL PHD STUDENTS CONFERENCE. Brno: MENDEL UNIV BRNO, 2019, p. 682-686. ISBN 978-80-7509-688-3. |
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@inproceedings{1729998,
author = {Vlčnovská, Marcela and Kuchynka, Michaela and Štossová, Aneta and Holcová Polanská, Hana and Vaculovicova, Marketa and Vaculovič, Tomáš and Masařík, Michal},
address = {Brno},
booktitle = {MENDELNET 2019: PROCEEDINGS OF 26TH INTERNATIONAL PHD STUDENTS CONFERENCE},
editor = {Cerkal, R Belcredi, NB Prokesova, L Pilatova, A},
keywords = {gold nanoparticles; LA-ICP-MS; immunochemistry; dot-blot; protein detection; p53},
howpublished = {elektronická verze "online"},
language = {eng},
location = {Brno},
isbn = {978-80-7509-688-3},
pages = {682-686},
publisher = {MENDEL UNIV BRNO},
title = {LA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysis},
url = {https://mendelnet.cz/artkey/mnt-201901-0131_LA-ICP-MS-as-a-sensitive-method-for-detection-of-nanoparticle-antibody-conjugates-in-immunochemistry-analysis.php?back=/magno/mnt/2019/mn1.php?secid=4},
year = {2019}
}
TY - JOUR
ID - 1729998
AU - Vlčnovská, Marcela - Kuchynka, Michaela - Štossová, Aneta - Holcová Polanská, Hana - Vaculovicova, Marketa - Vaculovič, Tomáš - Masařík, Michal
PY - 2019
TI - LA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysis
PB - MENDEL UNIV BRNO
CY - Brno
SN - 9788075096883
KW - gold nanoparticles
KW - LA-ICP-MS
KW - immunochemistry
KW - dot-blot
KW - protein detection
KW - p53
UR - https://mendelnet.cz/artkey/mnt-201901-0131_LA-ICP-MS-as-a-sensitive-method-for-detection-of-nanoparticle-antibody-conjugates-in-immunochemistry-analysis.php?back=/magno/mnt/2019/mn1.php?secid=4
L2 - https://mendelnet.cz/artkey/mnt-201901-0131_LA-ICP-MS-as-a-sensitive-method-for-detection-of-nanoparticle-antibody-conjugates-in-immunochemistry-analysis.php?back=/magno/mnt/2019/mn1.php?secid=4
N2 - Laser ablation followed by inductively coupled plasma mass spectrometry is an analytical method suitable for low concentration measurement of elements in a sample. This can be used not only for detection of elemental distribution within a sample but also as a detection means for immunoanalytical techniques using nanoparticle-antibody conjugates as recognition elements. The aim of this work was to prepare a conjugate of 10nm gold nanoparticles with anti-p53 antibody, to verify the antigen binding ability, and to use this construct for identification of p53 in a real sample of cell lysate. It has been experimentally verified that it is possible to use nanoparticle-modified antibodies for immunochemical analysis coupled to LA-ICP-MS detection. However, the nonspecific sorption of nanoparticles on the sample surface needs to be minimized by optimization of the blocking step.
ER -
VLČNOVSKÁ, Marcela, Michaela KUCHYNKA, Aneta ŠTOSSOVÁ, Hana HOLCOVÁ POLANSKÁ, Marketa VACULOVICOVA, Tomáš VACULOVIČ and Michal MASAŘÍK. LA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysis. Online. In Cerkal, R Belcredi, NB Prokesova, L Pilatova, A. \textit{MENDELNET 2019: PROCEEDINGS OF 26TH INTERNATIONAL PHD STUDENTS CONFERENCE}. Brno: MENDEL UNIV BRNO, 2019, p.~682-686. ISBN~978-80-7509-688-3.
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