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@inproceedings{1731040,
author = {Pilat, Zdenek and Jezek, Jan and Kizovsky, Martin and Klementova, Tereza and Kratky, Stanislav and Sobota, Jaroslav and Samek, Ota and Zemanek, Pavel and Buryška, Tomáš and Damborský, Jiří and Prokop, Zbyněk},
address = {New York},
booktitle = {2019 PHOTONICS & ELECTROMAGNETICS RESEARCH SYMPOSIUM - SPRING (PIERS-SPRING)},
doi = {http://dx.doi.org/10.1109/PIERS-Spring46901.2019.9017521},
keywords = {Raman Spectroscopy; 4-Aminothiophenol; Plasmonics},
howpublished = {tištěná verze "print"},
language = {eng},
location = {New York},
isbn = {978-1-7281-3403-1},
pages = {1301-1309},
publisher = {IEEE},
title = {Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring},
url = {https://ieeexplore.ieee.org/document/9017521},
year = {2019}
}
TY - JOUR
ID - 1731040
AU - Pilat, Zdenek - Jezek, Jan - Kizovsky, Martin - Klementova, Tereza - Kratky, Stanislav - Sobota, Jaroslav - Samek, Ota - Zemanek, Pavel - Buryška, Tomáš - Damborský, Jiří - Prokop, Zbyněk
PY - 2019
TI - Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring
PB - IEEE
CY - New York
SN - 9781728134031
KW - Raman Spectroscopy
KW - 4-Aminothiophenol
KW - Plasmonics
UR - https://ieeexplore.ieee.org/document/9017521
L2 - https://ieeexplore.ieee.org/document/9017521
N2 - Optofluidics, a research discipline combining optics with microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples e.g. for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in millimolar and even submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes.
ER -
PILAT, Zdenek, Jan JEZEK, Martin KIZOVSKY, Tereza KLEMENTOVA, Stanislav KRATKY, Jaroslav SOBOTA, Ota SAMEK, Pavel ZEMANEK, Tomáš BURYŠKA, Jiří DAMBORSKÝ a Zbyněk PROKOP. Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring. In \textit{2019 PHOTONICS \&{}amp; ELECTROMAGNETICS RESEARCH SYMPOSIUM - SPRING (PIERS-SPRING)}. New York: IEEE, 2019, s.~1301-1309. ISBN~978-1-7281-3403-1. Dostupné z: https://dx.doi.org/10.1109/PIERS-Spring46901.2019.9017521.
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