Surface-enhanced Raman Spectroscopy in Microfluidic Chips for
Directed Evolution of Enzymes and Environmental Monitoring

D 2019

Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring

PILAT, Zdenek, Jan JEZEK, Martin KIZOVSKY, Tereza KLEMENTOVA, Stanislav KRATKY et. al.

Základní údaje

Originální název

Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring

Autoři

PILAT, Zdenek, Jan JEZEK, Martin KIZOVSKY, Tereza KLEMENTOVA, Stanislav KRATKY, Jaroslav SOBOTA, Ota SAMEK, Pavel ZEMANEK, Tomáš BURYŠKA (203 Česká republika, domácí), Jiří DAMBORSKÝ (203 Česká republika, domácí) a Zbyněk PROKOP (203 Česká republika, domácí)

Vydání

New York, 2019 PHOTONICS & ELECTROMAGNETICS RESEARCH SYMPOSIUM - SPRING (PIERS-SPRING), od s. 1301-1309, 9 s. 2019

Nakladatel

IEEE

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

URL

Kód RIV

RIV/00216224:14310/19:00114704

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-1-7281-3403-1

ISSN

DOI

http://dx.doi.org/10.1109/PIERS-Spring46901.2019.9017521

UT WoS

000550769301050

Klíčová slova anglicky

Raman Spectroscopy; 4-Aminothiophenol; Plasmonics

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 4. 2021 13:07, Mgr. Marie Šípková, DiS.

Anotace

V originále

Optofluidics, a research discipline combining optics with microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples e.g. for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in millimolar and even submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes.

Návaznosti

GA16-07965S, projekt VaV

Název: Řízená evoluce dynamických elementů v enzymech s využitím mikrofluidních čipů

Investor: Grantová agentura ČR, Řízená evoluce dynamických elementů v enzymech s využitím mikrofluidních čipů

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

LO1214, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum pro výzkum toxických látek v prostředí

Citovat

PILAT, Zdenek, Jan JEZEK, Martin KIZOVSKY, Tereza KLEMENTOVA, Stanislav KRATKY, Jaroslav SOBOTA, Ota SAMEK, Pavel ZEMANEK, Tomáš BURYŠKA, Jiří DAMBORSKÝ a Zbyněk PROKOP. Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring. In 2019 PHOTONICS & ELECTROMAGNETICS RESEARCH SYMPOSIUM - SPRING (PIERS-SPRING). New York: IEEE, 2019, s. 1301-1309. ISBN 978-1-7281-3403-1. Dostupné z: https://dx.doi.org/10.1109/PIERS-Spring46901.2019.9017521.

@inproceedings{1731040,
   author = {Pilat, Zdenek and Jezek, Jan and Kizovsky, Martin and Klementova, Tereza and Kratky, Stanislav and Sobota, Jaroslav and Samek, Ota and Zemanek, Pavel and Buryška, Tomáš and Damborský, Jiří and Prokop, Zbyněk},
   address = {New York},
   booktitle = {2019 PHOTONICS & ELECTROMAGNETICS RESEARCH SYMPOSIUM - SPRING (PIERS-SPRING)},
   doi = {http://dx.doi.org/10.1109/PIERS-Spring46901.2019.9017521},
   keywords = {Raman Spectroscopy; 4-Aminothiophenol; Plasmonics},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {New York},
   isbn = {978-1-7281-3403-1},
   pages = {1301-1309},
   publisher = {IEEE},
   title = {Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring},
   url = {https://ieeexplore.ieee.org/document/9017521},
   year = {2019}
}

TY  - JOUR
ID  - 1731040
AU  - Pilat, Zdenek - Jezek, Jan - Kizovsky, Martin - Klementova, Tereza - Kratky, Stanislav - Sobota, Jaroslav - Samek, Ota - Zemanek, Pavel - Buryška, Tomáš - Damborský, Jiří - Prokop, Zbyněk
PY  - 2019
TI  - Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring
PB  - IEEE
CY  - New York
SN  - 9781728134031
KW  - Raman Spectroscopy
KW  - 4-Aminothiophenol
KW  - Plasmonics
UR  - https://ieeexplore.ieee.org/document/9017521
L2  - https://ieeexplore.ieee.org/document/9017521
N2  - Optofluidics, a research discipline combining optics with microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples e.g. for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in millimolar and even submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes.
ER  -

PILAT, Zdenek, Jan JEZEK, Martin KIZOVSKY, Tereza KLEMENTOVA, Stanislav KRATKY, Jaroslav SOBOTA, Ota SAMEK, Pavel ZEMANEK, Tomáš BURYŠKA, Jiří DAMBORSKÝ a Zbyněk PROKOP. Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring. In \textit{2019 PHOTONICS \&{}amp; ELECTROMAGNETICS RESEARCH SYMPOSIUM - SPRING (PIERS-SPRING)}. New York: IEEE, 2019, s.~1301-1309. ISBN~978-1-7281-3403-1. Dostupné z: https://dx.doi.org/10.1109/PIERS-Spring46901.2019.9017521.

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