OUTLÁ, Martina, Jan SPACEK, Daniel RENCIUK and Miroslav FOJTA. Study of synthetic oligonucleotides containing guanine by circular dichroism spectroscopy and voltammetry on a pyrolytic graphite electrode. In Nesmerak, K. PROCEEDINGS OF THE 15TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY. PRAGUE 2: CHARLES UNIV, FAC SCIENCE, 2019, p. 232-237. ISBN 978-80-7444-068-7.
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Basic information
Original name Study of synthetic oligonucleotides containing guanine by circular dichroism spectroscopy and voltammetry on a pyrolytic graphite electrode
Authors OUTLÁ, Martina (203 Czech Republic, belonging to the institution), Jan SPACEK, Daniel RENCIUK and Miroslav FOJTA (203 Czech Republic, belonging to the institution).
Edition PRAGUE 2, PROCEEDINGS OF THE 15TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY, p. 232-237, 6 pp. 2019.
Publisher CHARLES UNIV, FAC SCIENCE
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10406 Analytical chemistry
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Publication form printed version "print"
RIV identification code RIV/00216224:14310/19:00117919
Organization unit Faculty of Science
ISBN 978-80-7444-068-7
UT WoS 000576787700038
Keywords in English circular dichroism spectroscopy; guanine; oxidation; voltammetry
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 20/1/2021 11:18.
Abstract
The possibility to study DNA quadruplex structures using voltammetric analysis of DNA hexamers was investigated using a pyrolytic graphite electrode in an acetate buffer (pH = 5.0). We have tested the influence of the hexamer sequence and DNA concentration their anodic oxidation. To verify the G quadruplex formation, or lack thereof, circular dichroism spectroscopy was used. Our results suggest that, on the pyrolytic graphite electrode, the guanine oxidation signals are not influenced by formation of G the quadruplex. Instead, the shape of the G oxidation multiple peak can be related to the primary structure of the oligonucleotide.
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