Basic Bioelement Contents in Anaerobic Intestinal
Sulfate-Reducing Bacteria

J 2021

Basic Bioelement Contents in Anaerobic Intestinal Sulfate-Reducing Bacteria

KUSHKEVYCH, Ivan, Daryna ABDULINA, Dani DORDEVIC, Monika ROZEHNALOVÁ, Monika VÍTĚZOVÁ et. al.

Základní údaje

Originální název

Basic Bioelement Contents in Anaerobic Intestinal Sulfate-Reducing Bacteria

Autoři

KUSHKEVYCH, Ivan (804 Ukrajina, garant, domácí), Daryna ABDULINA, Dani DORDEVIC, Monika ROZEHNALOVÁ, Monika VÍTĚZOVÁ (203 Česká republika, domácí), Martin ČERNÝ (203 Česká republika, domácí), Pavel SVOBODA a Simon K.-M. R. RITTMANN

Vydání

Applied Sciences, Basel, MDPI, 2021, 2076-3417

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.838

Kód RIV

RIV/00216224:14310/21:00121033

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/app11031152

UT WoS

000614950800001

Klíčová slova anglicky

trace metals; hydrogen sulfide; toxicity; cell-free extracts; ulcerative colitis

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 5. 2021 11:50, Mgr. Marie Šípková, DiS.

Anotace

V originále

The monitoring of trace metals in microbial cells is relevant for diagnosis of inflammatory bowel disease (IBD). Sulfate-reducing bacteria (SRB) represent an important factor in the IBD development. The content of trace metals in bacterial cells may reflect the functioning of the enzyme systems and the environmental impact on the occurrence of SRB. The aim of our research was to compare the content of trace elements in the cells of SRB cultures isolated from fecal samples of patients with IBD and healthy people. The contents of 11 chemical elements in the bacterial cells of SRB were analyzed by the inductively coupled plasma-mass-spectrometry (ICP-MS) method. Significant changes in the content of calcium, zinc, magnesium, potassium, and iron were observed in patients with IBD compared to healthy individuals. Through a principal component analysis (PCA), a total variability of 67.3% in the difference between the samples was explained. The main factors influencing the total variability in the bacterial cells of SRB isolated from patients suffering from IBD were the content of the micro- and trace elements, such as manganese (with power 0.87), magnesium and cobalt (0.86), calcium (0.84), molybdenum (0.81), and iron (0.78). Such changes in the elemental composition of SRB under different conditions of existence in the host may indicate adaptive responses of the microorganisms, including the inclusion of oxidative stress systems, which can lead to changes in SRB metabolism and the manifestation of parameters of IBD in humans. The use of PCA might make it possible in the future to predict the development and ratio of SRB in patients with various diseases.

Návaznosti

MUNI/A/1425/2020, interní kód MU

Název: Podpora výzkumné činnosti studentů Mikrobiologie

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů Mikrobiologie

Citovat

KUSHKEVYCH, Ivan, Daryna ABDULINA, Dani DORDEVIC, Monika ROZEHNALOVÁ, Monika VÍTĚZOVÁ, Martin ČERNÝ, Pavel SVOBODA a Simon K.-M. R. RITTMANN. Basic Bioelement Contents in Anaerobic Intestinal Sulfate-Reducing Bacteria. Applied Sciences. Basel: MDPI, 2021, roč. 11, č. 3, s. 1-15. ISSN 2076-3417. Dostupné z: https://dx.doi.org/10.3390/app11031152.

@article{1734419,
   author = {Kushkevych, Ivan and Abdulina, Daryna and Dordevic, Dani and Rozehnalová, Monika and Vítězová, Monika and Černý, Martin and Svoboda, Pavel and Rittmann, Simon K.andM. R.},
   article_location = {Basel},
   article_number = {3},
   doi = {http://dx.doi.org/10.3390/app11031152},
   keywords = {trace metals; hydrogen sulfide; toxicity; cell-free extracts; ulcerative colitis},
   language = {eng},
   issn = {2076-3417},
   journal = {Applied Sciences},
   title = {Basic Bioelement Contents in Anaerobic Intestinal Sulfate-Reducing Bacteria},
   url = {https://doi.org/10.3390/app11031152},
   volume = {11},
   year = {2021}
}

TY  - JOUR
ID  - 1734419
AU  - Kushkevych, Ivan - Abdulina, Daryna - Dordevic, Dani - Rozehnalová, Monika - Vítězová, Monika - Černý, Martin - Svoboda, Pavel - Rittmann, Simon K.-M. R.
PY  - 2021
TI  - Basic Bioelement Contents in Anaerobic Intestinal Sulfate-Reducing Bacteria
JF  - Applied Sciences
VL  - 11
IS  - 3
SP  - 1-15
EP  - 1-15
PB  - MDPI
SN  - 20763417
KW  - trace metals
KW  - hydrogen sulfide
KW  - toxicity
KW  - cell-free extracts
KW  - ulcerative colitis
UR  - https://doi.org/10.3390/app11031152
L2  - https://doi.org/10.3390/app11031152
N2  - The monitoring of trace metals in microbial cells is relevant for diagnosis of inflammatory bowel disease (IBD). Sulfate-reducing bacteria (SRB) represent an important factor in the IBD development. The content of trace metals in bacterial cells may reflect the functioning of the enzyme systems and the environmental impact on the occurrence of SRB. The aim of our research was to compare the content of trace elements in the cells of SRB cultures isolated from fecal samples of patients with IBD and healthy people. The contents of 11 chemical elements in the bacterial cells of SRB were analyzed by the inductively coupled plasma-mass-spectrometry (ICP-MS) method. Significant changes in the content of calcium, zinc, magnesium, potassium, and iron were observed in patients with IBD compared to healthy individuals. Through a principal component analysis (PCA), a total variability of 67.3% in the difference between the samples was explained. The main factors influencing the total variability in the bacterial cells of SRB isolated from patients suffering from IBD were the content of the micro- and trace elements, such as manganese (with power 0.87), magnesium and cobalt (0.86), calcium (0.84), molybdenum (0.81), and iron (0.78). Such changes in the elemental composition of SRB under different conditions of existence in the host may indicate adaptive responses of the microorganisms, including the inclusion of oxidative stress systems, which can lead to changes in SRB metabolism and the manifestation of parameters of IBD in humans. The use of PCA might make it possible in the future to predict the development and ratio of SRB in patients with various diseases.
ER  -

KUSHKEVYCH, Ivan, Daryna ABDULINA, Dani DORDEVIC, Monika ROZEHNALOVÁ, Monika VÍTĚZOVÁ, Martin ČERNÝ, Pavel SVOBODA a Simon K.-M. R. RITTMANN. Basic Bioelement Contents in Anaerobic Intestinal Sulfate-Reducing Bacteria. \textit{Applied Sciences}. Basel: MDPI, 2021, roč.~11, č.~3, s.~1-15. ISSN~2076-3417. Dostupné z: https://dx.doi.org/10.3390/app11031152.

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