Detailed Information on Publication Record
2021
Basic Bioelement Contents in Anaerobic Intestinal Sulfate-Reducing Bacteria
KUSHKEVYCH, Ivan, Daryna ABDULINA, Dani DORDEVIC, Monika ROZEHNALOVÁ, Monika VÍTĚZOVÁ et. al.Basic information
Original name
Basic Bioelement Contents in Anaerobic Intestinal Sulfate-Reducing Bacteria
Authors
KUSHKEVYCH, Ivan (804 Ukraine, guarantor, belonging to the institution), Daryna ABDULINA, Dani DORDEVIC, Monika ROZEHNALOVÁ, Monika VÍTĚZOVÁ (203 Czech Republic, belonging to the institution), Martin ČERNÝ (203 Czech Republic, belonging to the institution), Pavel SVOBODA and Simon K.-M. R. RITTMANN
Edition
Applied Sciences, Basel, MDPI, 2021, 2076-3417
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10606 Microbiology
Country of publisher
Switzerland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 2.838
RIV identification code
RIV/00216224:14310/21:00121033
Organization unit
Faculty of Science
UT WoS
000614950800001
Keywords in English
trace metals; hydrogen sulfide; toxicity; cell-free extracts; ulcerative colitis
Tags
Tags
International impact, Reviewed
Změněno: 19/5/2021 11:50, Mgr. Marie Šípková, DiS.
Abstract
V originále
The monitoring of trace metals in microbial cells is relevant for diagnosis of inflammatory bowel disease (IBD). Sulfate-reducing bacteria (SRB) represent an important factor in the IBD development. The content of trace metals in bacterial cells may reflect the functioning of the enzyme systems and the environmental impact on the occurrence of SRB. The aim of our research was to compare the content of trace elements in the cells of SRB cultures isolated from fecal samples of patients with IBD and healthy people. The contents of 11 chemical elements in the bacterial cells of SRB were analyzed by the inductively coupled plasma-mass-spectrometry (ICP-MS) method. Significant changes in the content of calcium, zinc, magnesium, potassium, and iron were observed in patients with IBD compared to healthy individuals. Through a principal component analysis (PCA), a total variability of 67.3% in the difference between the samples was explained. The main factors influencing the total variability in the bacterial cells of SRB isolated from patients suffering from IBD were the content of the micro- and trace elements, such as manganese (with power 0.87), magnesium and cobalt (0.86), calcium (0.84), molybdenum (0.81), and iron (0.78). Such changes in the elemental composition of SRB under different conditions of existence in the host may indicate adaptive responses of the microorganisms, including the inclusion of oxidative stress systems, which can lead to changes in SRB metabolism and the manifestation of parameters of IBD in humans. The use of PCA might make it possible in the future to predict the development and ratio of SRB in patients with various diseases.
Links
| MUNI/A/1425/2020, interní kód MU |
|