Three new species of Cytauxzoon in European wild felids

J 2021

Three new species of Cytauxzoon in European wild felids

PANAIT, Luciana Catalina, Andrei Daniel MIHALCA, David MODRÝ, Jana JURÁNKOVÁ, Angela Monica IONICA et. al.

Základní údaje

Originální název

Three new species of Cytauxzoon in European wild felids

Autoři

PANAIT, Luciana Catalina, Andrei Daniel MIHALCA, David MODRÝ (203 Česká republika, domácí), Jana JURÁNKOVÁ, Angela Monica IONICA, Georgiana DEAK, Calin Mircea GHERMAN, Mike HEDDERGOTT, Adnan HODZIC, Fabrizia VERONESI, Mason REICHARD, Elliott A. ZIEMAN, Clayton K. NIELSEN, Francisco Agustín JIMÉNEZ-RUIZ a Kristýna HRAZDILOVÁ

Vydání

Veterinary Parasitology, Amsterdam, Elsevier, 2021, 0304-4017

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10613 Zoology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.821

Kód RIV

RIV/00216224:14310/21:00121035

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.vetpar.2021.109344

UT WoS

000674658700007

Klíčová slova anglicky

Cytauxzoon; European wild felids; Genotypes; Mitochondrial genes; Piroplasmids; 18S rDNA

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 8. 2021 10:00, Mgr. Marie Šípková, DiS.

Anotace

V originále

Protists of the genus Cytauxzoon infect a wide variety of wild and domestic felids worldwide. While the American Cytauxzoon felis has been well described, data on the European isolates of Cytauxzoon are still scant. The aim of the current study was to determine the genetic diversity of European Cytauxzoon spp. in wild felids across Europe by analyzing one nuclear and two mitochondrial genes, along with representative complete mitochondrial genomes. Overall, 106 biological samples from wild felids (92 from Felis silvestris and 14 from Lynx lynx) from Germany, Romania, Czech Republic, and Luxembourg were collected and screened for the presence of Cytauxzoon spp. using nested PCR protocols, targeting the highly conserved 18S rDNA, mitochondrial cytochrome b (CytB) and cytochrome c oxidase subunit I (COI) genes. Furthermore, 18 previously confirmed wild felid biological samples from Europe, and comparative material from USA positive for C. felis, were included in the study. In 18S rDNA sequences analyses, Cytauxzoon spp. from felids formed two separate clades of New World and Old World isolates, with a low inner diversity of the European clade. In contrast to 18S rDNA, the phylogenetic analyses of CytB and COI genes affirmatively revealed three highly supported clades, resulting in three defined genotypes. Similar intra- and interspecific variability of CytB and COI genes was observed in the case of different Babesia spp. Considering geography, host species and analyses of three genes, we conclude that the three detected genotypes of Cytauxzoon in European wild felids represent three new species, which we herein describe.

Citovat

PANAIT, Luciana Catalina, Andrei Daniel MIHALCA, David MODRÝ, Jana JURÁNKOVÁ, Angela Monica IONICA, Georgiana DEAK, Calin Mircea GHERMAN, Mike HEDDERGOTT, Adnan HODZIC, Fabrizia VERONESI, Mason REICHARD, Elliott A. ZIEMAN, Clayton K. NIELSEN, Francisco Agustín JIMÉNEZ-RUIZ a Kristýna HRAZDILOVÁ. Three new species of Cytauxzoon in European wild felids. Veterinary Parasitology. Amsterdam: Elsevier, 2021, roč. 290, February, s. "109344", 11 s. ISSN 0304-4017. Dostupné z: https://dx.doi.org/10.1016/j.vetpar.2021.109344.

@article{1734462,
   author = {Panait, Luciana Catalina and Mihalca, Andrei Daniel and Modrý, David and Juránková, Jana and Ionica, Angela Monica and Deak, Georgiana and Gherman, Calin Mircea and Heddergott, Mike and Hodzic, Adnan and Veronesi, Fabrizia and Reichard, Mason and Zieman, Elliott A. and Nielsen, Clayton K. and JiménezandRuiz, Francisco Agustín and Hrazdilová, Kristýna},
   article_location = {Amsterdam},
   article_number = {February},
   doi = {http://dx.doi.org/10.1016/j.vetpar.2021.109344},
   keywords = {Cytauxzoon; European wild felids; Genotypes; Mitochondrial genes; Piroplasmids; 18S rDNA},
   language = {eng},
   issn = {0304-4017},
   journal = {Veterinary Parasitology},
   title = {Three new species of Cytauxzoon in European wild felids},
   url = {https://doi.org/10.1016/j.vetpar.2021.109344},
   volume = {290},
   year = {2021}
}

TY  - JOUR
ID  - 1734462
AU  - Panait, Luciana Catalina - Mihalca, Andrei Daniel - Modrý, David - Juránková, Jana - Ionica, Angela Monica - Deak, Georgiana - Gherman, Calin Mircea - Heddergott, Mike - Hodzic, Adnan - Veronesi, Fabrizia - Reichard, Mason - Zieman, Elliott A. - Nielsen, Clayton K. - Jiménez-Ruiz, Francisco Agustín - Hrazdilová, Kristýna
PY  - 2021
TI  - Three new species of Cytauxzoon in European wild felids
JF  - Veterinary Parasitology
VL  - 290
IS  - February
SP  - "109344"
EP  - "109344"
PB  - Elsevier
SN  - 03044017
KW  - Cytauxzoon
KW  - European wild felids
KW  - Genotypes
KW  - Mitochondrial genes
KW  - Piroplasmids
KW  - 18S rDNA
UR  - https://doi.org/10.1016/j.vetpar.2021.109344
L2  - https://doi.org/10.1016/j.vetpar.2021.109344
N2  - Protists of the genus Cytauxzoon infect a wide variety of wild and domestic felids worldwide. While the American Cytauxzoon felis has been well described, data on the European isolates of Cytauxzoon are still scant. The aim of the current study was to determine the genetic diversity of European Cytauxzoon spp. in wild felids across Europe by analyzing one nuclear and two mitochondrial genes, along with representative complete mitochondrial genomes. Overall, 106 biological samples from wild felids (92 from Felis silvestris and 14 from Lynx lynx) from Germany, Romania, Czech Republic, and Luxembourg were collected and screened for the presence of Cytauxzoon spp. using nested PCR protocols, targeting the highly conserved 18S rDNA, mitochondrial cytochrome b (CytB) and cytochrome c oxidase subunit I (COI) genes. Furthermore, 18 previously confirmed wild felid biological samples from Europe, and comparative material from USA positive for C. felis, were included in the study. In 18S rDNA sequences analyses, Cytauxzoon spp. from felids formed two separate clades of New World and Old World isolates, with a low inner diversity of the European clade. In contrast to 18S rDNA, the phylogenetic analyses of CytB and COI genes affirmatively revealed three highly supported clades, resulting in three defined genotypes. Similar intra- and interspecific variability of CytB and COI genes was observed in the case of different Babesia spp. Considering geography, host species and analyses of three genes, we conclude that the three detected genotypes of Cytauxzoon in European wild felids represent three new species, which we herein describe.
ER  -

PANAIT, Luciana Catalina, Andrei Daniel MIHALCA, David MODRÝ, Jana JURÁNKOVÁ, Angela Monica IONICA, Georgiana DEAK, Calin Mircea GHERMAN, Mike HEDDERGOTT, Adnan HODZIC, Fabrizia VERONESI, Mason REICHARD, Elliott A. ZIEMAN, Clayton K. NIELSEN, Francisco Agustín JIMÉNEZ-RUIZ a Kristýna HRAZDILOVÁ. Three new species of Cytauxzoon in European wild felids. \textit{Veterinary Parasitology}. Amsterdam: Elsevier, 2021, roč.~290, February, s.~''109344'', 11 s. ISSN~0304-4017. Dostupné z: https://dx.doi.org/10.1016/j.vetpar.2021.109344.

Podrobný výpis o publikaci