Myeloperoxidase mediated alteration of endothelial function is
dependent on its cationic charge

J 2021

Myeloperoxidase mediated alteration of endothelial function is dependent on its cationic charge

KOLÁŘOVÁ, Hana, Jan VÍTEČEK, Anna ČERNÁ, Marek ČERNÍK, Jan PŘIBYL et. al.

Základní údaje

Originální název

Myeloperoxidase mediated alteration of endothelial function is dependent on its cationic charge

Autoři

KOLÁŘOVÁ, Hana, Jan VÍTEČEK, Anna ČERNÁ, Marek ČERNÍK (203 Česká republika), Jan PŘIBYL (203 Česká republika, domácí), Petr SKLÁDAL (203 Česká republika, domácí), David POTĚŠIL (203 Česká republika, domácí), Ivana IHNATOVÁ (703 Slovensko, domácí), Zbyněk ZDRÁHAL (203 Česká republika, domácí), Aleš HAMPL (203 Česká republika, domácí), Anna KLINKE a Lukáš KUBALA (garant)

Vydání

Free Radical Biology and Medicine, New York, Elsevier, 2021, 0891-5849

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 8.101

Kód RIV

RIV/00216224:14740/21:00121039

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.freeradbiomed.2020.11.008

UT WoS

000618526500002

Klíčová slova anglicky

Myeloperoxidase; Inflammation; Cardiovascular diseases; Glycocalyx; Endothelial cells; Proteomic analysis; Glycosaminoglycan; Vascular inflammation

Štítky

14110517, 14119612, CF NANO, CF PROT, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 10. 2024 14:13, Ing. Martina Blahová

Anotace

V originále

Endothelial cell (EC) glycocalyx (GLX) comprise a multicomponent layer of proteoglycans and glycoproteins. Alteration of its integrity contributes to chronic vascular inflammation and leads to the development of cardiovascular diseases. Myeloperoxidase (MPO), a highly abundant enzyme released by polymorphonuclear neutrophils, binds to the GLX and deleteriously affects vascular EC functions. The focus of this study was to elucidate the mechanisms of MPO-mediated alteration of GLX molecules, and to unravel subsequent changes in endothelial integrity and function. MPO binding to GLX of human ECs and subsequent internalization was mediated by cell surface heparan sulfate chains. Moreover, interaction of MPO, which is carrying a cationic charge, with anionic glycosaminoglycans (GAGs) resulted in reduction of their relative charge. By means of micro-viscometry and atomic force microscopy, we disclosed that MPO can crosslink GAG chains. MPO-dependent modulation of GLX structure was further supported by alteration of wheat germ agglutinin staining. Increased expression of ICAM-1 documented endothelial cell activation by both catalytically active and also inactive MPO. Furthermore, MPO increased vascular permeability connected with reorganization of intracellular junctions, however, this was dependent on MPO's catalytic activity. Novel proteins interacting with MPO during transcytosis were identified by proteomic analysis. Altogether, these findings provide evidence that MPO through interaction with GAGs modulates overall charge of the GLX, causing modification of its structure and thus affecting EC function. Importantly, our results also suggest a number of proteins interacting with MPO that possess a variety of cellular localizations and functions.

Návaznosti

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

KOLÁŘOVÁ, Hana, Jan VÍTEČEK, Anna ČERNÁ, Marek ČERNÍK, Jan PŘIBYL, Petr SKLÁDAL, David POTĚŠIL, Ivana IHNATOVÁ, Zbyněk ZDRÁHAL, Aleš HAMPL, Anna KLINKE a Lukáš KUBALA. Myeloperoxidase mediated alteration of endothelial function is dependent on its cationic charge. Free Radical Biology and Medicine. New York: Elsevier, 2021, roč. 162, January 2021, s. 14-26. ISSN 0891-5849. Dostupné z: https://dx.doi.org/10.1016/j.freeradbiomed.2020.11.008.

@article{1734582,
   author = {Kolářová, Hana and Víteček, Jan and Černá, Anna and Černík, Marek and Přibyl, Jan and Skládal, Petr and Potěšil, David and Ihnatová, Ivana and Zdráhal, Zbyněk and Hampl, Aleš and Klinke, Anna and Kubala, Lukáš},
   article_location = {New York},
   article_number = {January 2021},
   doi = {http://dx.doi.org/10.1016/j.freeradbiomed.2020.11.008},
   keywords = {Myeloperoxidase; Inflammation; Cardiovascular diseases; Glycocalyx; Endothelial cells; Proteomic analysis; Glycosaminoglycan; Vascular inflammation},
   language = {eng},
   issn = {0891-5849},
   journal = {Free Radical Biology and Medicine},
   title = {Myeloperoxidase mediated alteration of endothelial function is dependent on its cationic charge},
   url = {https://doi.org/10.1016/j.freeradbiomed.2020.11.008},
   volume = {162},
   year = {2021}
}

TY  - JOUR
ID  - 1734582
AU  - Kolářová, Hana - Víteček, Jan - Černá, Anna - Černík, Marek - Přibyl, Jan - Skládal, Petr - Potěšil, David - Ihnatová, Ivana - Zdráhal, Zbyněk - Hampl, Aleš - Klinke, Anna - Kubala, Lukáš
PY  - 2021
TI  - Myeloperoxidase mediated alteration of endothelial function is dependent on its cationic charge
JF  - Free Radical Biology and Medicine
VL  - 162
IS  - January 2021
SP  - 14-26
EP  - 14-26
PB  - Elsevier
SN  - 08915849
KW  - Myeloperoxidase
KW  - Inflammation
KW  - Cardiovascular diseases
KW  - Glycocalyx
KW  - Endothelial cells
KW  - Proteomic analysis
KW  - Glycosaminoglycan
KW  - Vascular inflammation
UR  - https://doi.org/10.1016/j.freeradbiomed.2020.11.008
L2  - https://doi.org/10.1016/j.freeradbiomed.2020.11.008
N2  - Endothelial cell (EC) glycocalyx (GLX) comprise a multicomponent layer of proteoglycans and glycoproteins. Alteration of its integrity contributes to chronic vascular inflammation and leads to the development of cardiovascular diseases. Myeloperoxidase (MPO), a highly abundant enzyme released by polymorphonuclear neutrophils, binds to the GLX and deleteriously affects vascular EC functions. The focus of this study was to elucidate the mechanisms of MPO-mediated alteration of GLX molecules, and to unravel subsequent changes in endothelial integrity and function. MPO binding to GLX of human ECs and subsequent internalization was mediated by cell surface heparan sulfate chains. Moreover, interaction of MPO, which is carrying a cationic charge, with anionic glycosaminoglycans (GAGs) resulted in reduction of their relative charge. By means of micro-viscometry and atomic force microscopy, we disclosed that MPO can crosslink GAG chains. MPO-dependent modulation of GLX structure was further supported by alteration of wheat germ agglutinin staining. Increased expression of ICAM-1 documented endothelial cell activation by both catalytically active and also inactive MPO. Furthermore, MPO increased vascular permeability connected with reorganization of intracellular junctions, however, this was dependent on MPO's catalytic activity. Novel proteins interacting with MPO during transcytosis were identified by proteomic analysis. Altogether, these findings provide evidence that MPO through interaction with GAGs modulates overall charge of the GLX, causing modification of its structure and thus affecting EC function. Importantly, our results also suggest a number of proteins interacting with MPO that possess a variety of cellular localizations and functions.
ER  -

KOLÁŘOVÁ, Hana, Jan VÍTEČEK, Anna ČERNÁ, Marek ČERNÍK, Jan PŘIBYL, Petr SKLÁDAL, David POTĚŠIL, Ivana IHNATOVÁ, Zbyněk ZDRÁHAL, Aleš HAMPL, Anna KLINKE a Lukáš KUBALA. Myeloperoxidase mediated alteration of endothelial function is dependent on its cationic charge. \textit{Free Radical Biology and Medicine}. New York: Elsevier, 2021, roč.~162, January 2021, s.~14-26. ISSN~0891-5849. Dostupné z: https://dx.doi.org/10.1016/j.freeradbiomed.2020.11.008.

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