Bacteriophage replication on permissive host cells in fused
silica capillary with nanostructured part as potential of
electrophoretic methods for developing phage applications

J 2021

Bacteriophage replication on permissive host cells in fused silica capillary with nanostructured part as potential of electrophoretic methods for developing phage applications

HORKÁ, Marie, Pavel KARÁSEK, Michal ROTH, Dana ŠTVERÁKOVÁ, Jiří ŠALPLACHTA et. al.

Základní údaje

Originální název

Bacteriophage replication on permissive host cells in fused silica capillary with nanostructured part as potential of electrophoretic methods for developing phage applications

Autoři

HORKÁ, Marie, Pavel KARÁSEK, Michal ROTH, Dana ŠTVERÁKOVÁ (203 Česká republika, domácí), Jiří ŠALPLACHTA, Filip RŮŽIČKA (203 Česká republika, domácí) a Roman PANTŮČEK (203 Česká republika, garant, domácí)

Vydání

Talanta, Amsterdam, Elsevier, 2021, 0039-9140

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.556

Kód RIV

RIV/00216224:14310/21:00118847

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.talanta.2020.121800

UT WoS

000600787800031

Klíčová slova anglicky

Capillary electrophoretic methods; Fused silica capillary with roughened part; Microliter sample volumes; Dynamic adhesion of bacterial cells; Staphylococcus aureus; Bacteriophage propagation

Štítky

14110113, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 2. 2021 11:03, Mgr. Marie Šípková, DiS.

Anotace

V originále

Phage therapy could offer a safe and effective alternative to antibiotic treatment of infections caused by Gram-positive bacterium Staphylococcus aureus that have emerged as a significant threat in hospital and community environment and is attracting growing interest among clinicians. The legislation process of approving the phage therapeutics by pharmaceutical authorities requires rapid analytical techniques for assessment of phage activity. Here, we present a three-step method for on-line monitoring the phage effect on bacterial cells dynamically adhered from microliter volumes of high conductivity matrix onto the inner surface of fused silica capillary with a part etched with supercritical water. Phage K1/420 particles of the Kayvirus genus generated by propagation on the host S. aureus cells together with the uninfected cells were concentrated, separated and detected using capillary electrophoretic methods. The phage interactions with selected S. aureus strains exhibiting differences in phage susceptibility were compared. The method allowed determination of the phage burst size and time of phage latent period in analyzed strains. Apart from enumeration of bacteriophages by the plaque assays, the proposed method is suitable for phage activity testing.

Návaznosti

GA18-13064S, projekt VaV

Název: Analýza interakcí mezi polyvalentním terapeutickým fágem druhu Twort-like a jeho hostitelem Staphylococcus aureus

Investor: Grantová agentura ČR, Analýza interakcí mezi polyvalentním terapeutickým fágem druhu Twort-like a jeho hostitelem Staphylococcus aureus

NV16-29916A, projekt VaV

Název: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

VI20172020069, projekt VaV

Název: Vývoj moderních instrumentálních metod pro rychlou detekci a identifikaci vybraných B-agens a toxinů (Akronym: VMIMRDIBT)

Investor: Ministerstvo vnitra ČR, Vývoj moderních instrumentálních metod pro rychlou detekci a identifikaci vybraných B-agens a toxinů

Citovat

HORKÁ, Marie, Pavel KARÁSEK, Michal ROTH, Dana ŠTVERÁKOVÁ, Jiří ŠALPLACHTA, Filip RŮŽIČKA a Roman PANTŮČEK. Bacteriophage replication on permissive host cells in fused silica capillary with nanostructured part as potential of electrophoretic methods for developing phage applications. Talanta. Amsterdam: Elsevier, 2021, roč. 224, March 2021, s. "121800-1"-"121800-8", 8 s. ISSN 0039-9140. Dostupné z: https://dx.doi.org/10.1016/j.talanta.2020.121800.

@article{1734916,
   author = {Horká, Marie and Karásek, Pavel and Roth, Michal and Štveráková, Dana and Šalplachta, Jiří and Růžička, Filip and Pantůček, Roman},
   article_location = {Amsterdam},
   article_number = {March 2021},
   doi = {http://dx.doi.org/10.1016/j.talanta.2020.121800},
   keywords = {Capillary electrophoretic methods; Fused silica capillary with roughened part; Microliter sample volumes; Dynamic adhesion of bacterial cells; Staphylococcus aureus; Bacteriophage propagation},
   language = {eng},
   issn = {0039-9140},
   journal = {Talanta},
   title = {Bacteriophage replication on permissive host cells in fused silica capillary with nanostructured part as potential of electrophoretic methods for developing phage applications},
   url = {https://doi.org/10.1016/j.talanta.2020.121800},
   volume = {224},
   year = {2021}
}

TY  - JOUR
ID  - 1734916
AU  - Horká, Marie - Karásek, Pavel - Roth, Michal - Štveráková, Dana - Šalplachta, Jiří - Růžička, Filip - Pantůček, Roman
PY  - 2021
TI  - Bacteriophage replication on permissive host cells in fused silica capillary with nanostructured part as potential of electrophoretic methods for developing phage applications
JF  - Talanta
VL  - 224
IS  - March 2021
SP  - "121800-1"-"121800-8"
EP  - "121800-1"-"121800-8"
PB  - Elsevier
SN  - 00399140
KW  - Capillary electrophoretic methods
KW  - Fused silica capillary with roughened part
KW  - Microliter sample volumes
KW  - Dynamic adhesion of bacterial cells
KW  - Staphylococcus aureus
KW  - Bacteriophage propagation
UR  - https://doi.org/10.1016/j.talanta.2020.121800
L2  - https://doi.org/10.1016/j.talanta.2020.121800
N2  - Phage therapy could offer a safe and effective alternative to antibiotic treatment of infections caused by Gram-positive bacterium Staphylococcus aureus that have emerged as a significant threat in hospital and community environment and is attracting growing interest among clinicians. The legislation process of approving the phage therapeutics by pharmaceutical authorities requires rapid analytical techniques for assessment of phage activity. Here, we present a three-step method for on-line monitoring the phage effect on bacterial cells dynamically adhered from microliter volumes of high conductivity matrix onto the inner surface of fused silica capillary with a part etched with supercritical water. Phage K1/420 particles of the Kayvirus genus generated by propagation on the host S. aureus cells together with the uninfected cells were concentrated, separated and detected using capillary electrophoretic methods. The phage interactions with selected S. aureus strains exhibiting differences in phage susceptibility were compared. The method allowed determination of the phage burst size and time of phage latent period in analyzed strains. Apart from enumeration of bacteriophages by the plaque assays, the proposed method is suitable for phage activity testing.
ER  -

HORKÁ, Marie, Pavel KARÁSEK, Michal ROTH, Dana ŠTVERÁKOVÁ, Jiří ŠALPLACHTA, Filip RŮŽIČKA a Roman PANTŮČEK. Bacteriophage replication on permissive host cells in fused silica capillary with nanostructured part as potential of electrophoretic methods for developing phage applications. \textit{Talanta}. Amsterdam: Elsevier, 2021, roč.~224, March 2021, s.~''121800-1''-''121800-8'', 8 s. ISSN~0039-9140. Dostupné z: https://dx.doi.org/10.1016/j.talanta.2020.121800.

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