Multiple In vitro biological effects of phenolic compounds from
Morus alba root bark

J 2020

Multiple In vitro biological effects of phenolic compounds from Morus alba root bark

ČULENOVÁ, Marie, Alice SYCHROVÁ, S. T. S. HASSAN, K. BERCHOVA-BIMOVA, P. SVOBODOVA et. al.

Základní údaje

Originální název

Multiple In vitro biological effects of phenolic compounds from Morus alba root bark

Autoři

ČULENOVÁ, Marie (203 Česká republika, garant, domácí), Alice SYCHROVÁ (203 Česká republika, domácí), S. T. S. HASSAN, K. BERCHOVA-BIMOVA, P. SVOBODOVA, A. HELCLOVA, H. MICHNOVA, J. HOSEK, H. VASILEV, Pavel SUCHÝ (203 Česká republika, domácí), Gabriela KUZMÍNOVÁ (203 Česká republika, domácí), Emil ŠVAJDLENKA (203 Česká republika, domácí), Jan GAJDZIOK (203 Česká republika, domácí), Alois ČÍŽEK (203 Česká republika, domácí), Václav SUCHÝ (203 Česká republika, domácí) a Karel ŠMEJKAL (203 Česká republika, domácí)

Vydání

JOURNAL OF ETHNOPHARMACOLOGY, CLARE, ELSEVIER IRELAND LTD, 2020, 0378-8741

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.360

Kód RIV

RIV/00216224:14160/20:00118192

Organizační jednotka

Farmaceutická fakulta

DOI

http://dx.doi.org/10.1016/j.jep.2019.112296

UT WoS

000503312800025

Klíčová slova anglicky

Antiviral; Antibacterial; Anti-inflammatory; Docking; Mulberry; Phenolic

Štítky

rivok, ÚFT, ÚFTo, ÚPL

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 2. 2021 08:19, Mgr. Hana Hurtová

Anotace

V originále

Ethnopharmacological relevance: Morus alba L. is used in traditional Chinese medicine for the treatment of various diseases, including bacterial infections and inflammation. As a rich source of phenolic compounds, the plant is an object of many phytochemical and pharmacological studies. Aim of the study: The aim of the study was to isolate and evaluate possible parallel antiviral, antibacterial, and anti-inflammatory activities of phenolic mulberry compounds. Materials and methods: Extensive chromatographic separation of mulberry root bark extract and in vitro biological screening of 26 constituents identified promising candidates for further pharmacological research. Selected compounds were screened for anti-infective and anti-inflammatory activities. Antiviral activity was determined by the plaque number reduction assay and by the titer reduction assay, antibacterial using broth microdilution method, and anti-inflammatory activity using COX Colorimetric inhibitor screening assay kit. One compound was evaluated in vivo in carrageenan-induced paw-edema in mice. Results: Five prenylated compounds 1, 2, 8, 9, and 11, together with a simple phenolic ester 13, exhibited inhibitory activity against the replication of herpes simplex virus 1 (HSV-1) or herpes simplex virus 2 (HSV-2), with IC50 values ranging from 0.64 to 1.93 mu g/mL, and EC50 values 0.93 and 1.61 mu g/mL. Molecular docking studies demonstrated the effects of the active compounds by targeting HSV-1 DNA polymerase and HSV-2 protease. In antibacterial assay, compounds 1, 4, 11, and 17 diminished the growth of all of the Gram-positive strains tested, with MIC values of 1-16 mu g/mL. The anti-inflammatory ability of several compounds to inhibit cyclooxygenase 2 (COX-2) was tested in vitro, and compound 16 displayed greater activity than the indomethacin, positive control. Mulberrofuran B (11) showed anti-inflammatory activity in vivo against carrageenan-induced paw-edema in mice. Conclusions: Experimental investigation showed promising antiviral, antibacterial, and/or anti-inflammatory activities of the phenolic mulberry constituents, often with multiple inhibitory effects that might be used as a potential source of new medicine.

Citovat

ČULENOVÁ, Marie, Alice SYCHROVÁ, S. T. S. HASSAN, K. BERCHOVA-BIMOVA, P. SVOBODOVA, A. HELCLOVA, H. MICHNOVA, J. HOSEK, H. VASILEV, Pavel SUCHÝ, Gabriela KUZMÍNOVÁ, Emil ŠVAJDLENKA, Jan GAJDZIOK, Alois ČÍŽEK, Václav SUCHÝ a Karel ŠMEJKAL. Multiple In vitro biological effects of phenolic compounds from Morus alba root bark. JOURNAL OF ETHNOPHARMACOLOGY. CLARE: ELSEVIER IRELAND LTD, 2020, roč. 248, č. 112296, s. 1-12. ISSN 0378-8741. Dostupné z: https://dx.doi.org/10.1016/j.jep.2019.112296.

@article{1743498,
   author = {Čulenová, Marie and Sychrová, Alice and Hassan, S. T. S. and BerchovaandBimova, K. and Svobodova, P. and Helclova, A. and Michnova, H. and Hosek, J. and Vasilev, H. and Suchý, Pavel and Kuzmínová, Gabriela and Švajdlenka, Emil and Gajdziok, Jan and Čížek, Alois and Suchý, Václav and Šmejkal, Karel},
   article_location = {CLARE},
   article_number = {112296},
   doi = {http://dx.doi.org/10.1016/j.jep.2019.112296},
   keywords = {Antiviral; Antibacterial; Anti-inflammatory; Docking; Mulberry; Phenolic},
   language = {eng},
   issn = {0378-8741},
   journal = {JOURNAL OF ETHNOPHARMACOLOGY},
   title = {Multiple In vitro biological effects of phenolic compounds from Morus alba root bark},
   url = {https://www.sciencedirect.com/science/article/pii/S0378874119337298?via%3Dihub},
   volume = {248},
   year = {2020}
}

TY  - JOUR
ID  - 1743498
AU  - Čulenová, Marie - Sychrová, Alice - Hassan, S. T. S. - Berchova-Bimova, K. - Svobodova, P. - Helclova, A. - Michnova, H. - Hosek, J. - Vasilev, H. - Suchý, Pavel - Kuzmínová, Gabriela - Švajdlenka, Emil - Gajdziok, Jan - Čížek, Alois - Suchý, Václav - Šmejkal, Karel
PY  - 2020
TI  - Multiple In vitro biological effects of phenolic compounds from Morus alba root bark
JF  - JOURNAL OF ETHNOPHARMACOLOGY
VL  - 248
IS  - 112296
SP  - 1-12
EP  - 1-12
PB  - ELSEVIER IRELAND LTD
SN  - 03788741
KW  - Antiviral
KW  - Antibacterial
KW  - Anti-inflammatory
KW  - Docking
KW  - Mulberry
KW  - Phenolic
UR  - https://www.sciencedirect.com/science/article/pii/S0378874119337298?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0378874119337298?via%3Dihub
N2  - Ethnopharmacological relevance: Morus alba L. is used in traditional Chinese medicine for the treatment of various diseases, including bacterial infections and inflammation. As a rich source of phenolic compounds, the plant is an object of many phytochemical and pharmacological studies. Aim of the study: The aim of the study was to isolate and evaluate possible parallel antiviral, antibacterial, and anti-inflammatory activities of phenolic mulberry compounds. Materials and methods: Extensive chromatographic separation of mulberry root bark extract and in vitro biological screening of 26 constituents identified promising candidates for further pharmacological research. Selected compounds were screened for anti-infective and anti-inflammatory activities. Antiviral activity was determined by the plaque number reduction assay and by the titer reduction assay, antibacterial using broth microdilution method, and anti-inflammatory activity using COX Colorimetric inhibitor screening assay kit. One compound was evaluated in vivo in carrageenan-induced paw-edema in mice. Results: Five prenylated compounds 1, 2, 8, 9, and 11, together with a simple phenolic ester 13, exhibited inhibitory activity against the replication of herpes simplex virus 1 (HSV-1) or herpes simplex virus 2 (HSV-2), with IC50 values ranging from 0.64 to 1.93 mu g/mL, and EC50 values 0.93 and 1.61 mu g/mL. Molecular docking studies demonstrated the effects of the active compounds by targeting HSV-1 DNA polymerase and HSV-2 protease. In antibacterial assay, compounds 1, 4, 11, and 17 diminished the growth of all of the Gram-positive strains tested, with MIC values of 1-16 mu g/mL. The anti-inflammatory ability of several compounds to inhibit cyclooxygenase 2 (COX-2) was tested in vitro, and compound 16 displayed greater activity than the indomethacin, positive control. Mulberrofuran B (11) showed anti-inflammatory activity in vivo against carrageenan-induced paw-edema in mice. Conclusions: Experimental investigation showed promising antiviral, antibacterial, and/or anti-inflammatory activities of the phenolic mulberry constituents, often with multiple inhibitory effects that might be used as a potential source of new medicine.
ER  -

ČULENOVÁ, Marie, Alice SYCHROVÁ, S. T. S. HASSAN, K. BERCHOVA-BIMOVA, P. SVOBODOVA, A. HELCLOVA, H. MICHNOVA, J. HOSEK, H. VASILEV, Pavel SUCHÝ, Gabriela KUZMÍNOVÁ, Emil ŠVAJDLENKA, Jan GAJDZIOK, Alois ČÍŽEK, Václav SUCHÝ a Karel ŠMEJKAL. Multiple In vitro biological effects of phenolic compounds from Morus alba root bark. \textit{JOURNAL OF ETHNOPHARMACOLOGY}. CLARE: ELSEVIER IRELAND LTD, 2020, roč.~248, č.~112296, s.~1-12. ISSN~0378-8741. Dostupné z: https://dx.doi.org/10.1016/j.jep.2019.112296.

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