YAP-TEAD1 control of cytoskeleton dynamics and intracellular
tension guides human pluripotent stem cell mesoderm
specification

J 2021

YAP-TEAD1 control of cytoskeleton dynamics and intracellular tension guides human pluripotent stem cell mesoderm specification

PAGLIARI, S., Vladimir VINARSKY, Fabiana MARTINO, A. R. PERESTRELO, J. O. DE LA CRUZ et. al.

Základní údaje

Originální název

YAP-TEAD1 control of cytoskeleton dynamics and intracellular tension guides human pluripotent stem cell mesoderm specification

Autoři

PAGLIARI, S., Vladimir VINARSKY (203 Česká republika), Fabiana MARTINO (380 Itálie, domácí), A. R. PERESTRELO, J. O. DE LA CRUZ, Guido CALUORI (380 Itálie, domácí), Jan VRBSKY (203 Česká republika), P. MOZETIC, A. POMPEIANO, A. ZANCLA, Sri Ranjani GANJI (356 Indie, domácí), Petr SKLÁDAL (203 Česká republika, domácí), Dan KYTYR (203 Česká republika), Zbyněk ZDRÁHAL (203 Česká republika, garant, domácí), G. GRASSI, M. SAMPAOLESI, A. RAINER a G. FORTE

Vydání

Cell Death and Differentiation, London, Springer Nature, 2021, 1350-9047

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 12.067

Kód RIV

RIV/00216224:14740/21:00118867

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1038/s41418-020-00643-5

UT WoS

000584874800002

Klíčová slova anglicky

NCK-INTERACTING KINASEHIPPO PATHWAYSIGNALING PATHWAYSELF-RENEWALSIZE-CONTROLORGAN SIZEYAPANGIOMOTINHOMEOSTASISDIFFERENTIATION

Štítky

14110513, CF PROT, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 10. 2024 14:30, Ing. Martina Blahová

Anotace

V originále

The tight regulation of cytoskeleton dynamics is required for a number of cellular processes, including migration, division and differentiation. YAP-TEAD respond to cell-cell interaction and to substrate mechanics and, among their downstream effects, prompt focal adhesion (FA) gene transcription, thus contributing to FA-cytoskeleton stability. This activity is key to the definition of adult cell mechanical properties and function. Its regulation and role in pluripotent stem cells are poorly understood. Human PSCs display a sustained basal YAP-driven transcriptional activity despite they grow in very dense colonies, indicating these cells are insensitive to contact inhibition. PSC inability to perceive cell-cell interactions can be restored by tampering with Tankyrase enzyme, thus favouring AMOT inhibition of YAP function. YAP-TEAD complex is promptly inactivated when germ layers are specified, and this event is needed to adjust PSC mechanical properties in response to physiological substrate stiffness. By providing evidence that YAP-TEAD1 complex targets key genes encoding for proteins involved in cytoskeleton dynamics, we suggest that substrate mechanics can direct PSC specification by influencing cytoskeleton arrangement and intracellular tension. We propose an aberrant activation of YAP-TEAD1 axis alters PSC potency by inhibiting cytoskeleton dynamics, thus paralyzing the changes in shape requested for the acquisition of the given phenotype.

Návaznosti

GBP206/12/G151, projekt VaV

Název: Centrum nových přístupů k bioanalýze a molekulární diagnostice

LM2015043, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

90043, velká výzkumná infrastruktura

Název: CIISB

Citovat

PAGLIARI, S., Vladimir VINARSKY, Fabiana MARTINO, A. R. PERESTRELO, J. O. DE LA CRUZ, Guido CALUORI, Jan VRBSKY, P. MOZETIC, A. POMPEIANO, A. ZANCLA, Sri Ranjani GANJI, Petr SKLÁDAL, Dan KYTYR, Zbyněk ZDRÁHAL, G. GRASSI, M. SAMPAOLESI, A. RAINER a G. FORTE. YAP-TEAD1 control of cytoskeleton dynamics and intracellular tension guides human pluripotent stem cell mesoderm specification. Cell Death and Differentiation. London: Springer Nature, 2021, roč. 28, č. 4, s. 1193-1207. ISSN 1350-9047. Dostupné z: https://dx.doi.org/10.1038/s41418-020-00643-5.

@article{1745296,
   author = {Pagliari, S. and Vinarsky, Vladimir and Martino, Fabiana and Perestrelo, A. R. and De La Cruz, J. O. and Caluori, Guido and Vrbsky, Jan and Mozetic, P. and Pompeiano, A. and Zancla, A. and Ganji, Sri Ranjani and Skládal, Petr and Kytyr, Dan and Zdráhal, Zbyněk and Grassi, G. and Sampaolesi, M. and Rainer, A. and Forte, G.},
   article_location = {London},
   article_number = {4},
   doi = {http://dx.doi.org/10.1038/s41418-020-00643-5},
   keywords = {NCK-INTERACTING KINASEHIPPO PATHWAYSIGNALING PATHWAYSELF-RENEWALSIZE-CONTROLORGAN SIZEYAPANGIOMOTINHOMEOSTASISDIFFERENTIATION},
   language = {eng},
   issn = {1350-9047},
   journal = {Cell Death and Differentiation},
   title = {YAP-TEAD1 control of cytoskeleton dynamics and intracellular tension guides human pluripotent stem cell mesoderm specification},
   url = {https://www.nature.com/articles/s41418-020-00643-5.pdf},
   volume = {28},
   year = {2021}
}

TY  - JOUR
ID  - 1745296
AU  - Pagliari, S. - Vinarsky, Vladimir - Martino, Fabiana - Perestrelo, A. R. - De La Cruz, J. O. - Caluori, Guido - Vrbsky, Jan - Mozetic, P. - Pompeiano, A. - Zancla, A. - Ganji, Sri Ranjani - Skládal, Petr - Kytyr, Dan - Zdráhal, Zbyněk - Grassi, G. - Sampaolesi, M. - Rainer, A. - Forte, G.
PY  - 2021
TI  - YAP-TEAD1 control of cytoskeleton dynamics and intracellular tension guides human pluripotent stem cell mesoderm specification
JF  - Cell Death and Differentiation
VL  - 28
IS  - 4
SP  - 1193-1207
EP  - 1193-1207
PB  - Springer Nature
SN  - 13509047
KW  - NCK-INTERACTING KINASEHIPPO PATHWAYSIGNALING PATHWAYSELF-RENEWALSIZE-CONTROLORGAN SIZEYAPANGIOMOTINHOMEOSTASISDIFFERENTIATION
UR  - https://www.nature.com/articles/s41418-020-00643-5.pdf
N2  - The tight regulation of cytoskeleton dynamics is required for a number of cellular processes, including migration, division and differentiation. YAP-TEAD respond to cell-cell interaction and to substrate mechanics and, among their downstream effects, prompt focal adhesion (FA) gene transcription, thus contributing to FA-cytoskeleton stability. This activity is key to the definition of adult cell mechanical properties and function. Its regulation and role in pluripotent stem cells are poorly understood. Human PSCs display a sustained basal YAP-driven transcriptional activity despite they grow in very dense colonies, indicating these cells are insensitive to contact inhibition. PSC inability to perceive cell-cell interactions can be restored by tampering with Tankyrase enzyme, thus favouring AMOT inhibition of YAP function. YAP-TEAD complex is promptly inactivated when germ layers are specified, and this event is needed to adjust PSC mechanical properties in response to physiological substrate stiffness. By providing evidence that YAP-TEAD1 complex targets key genes encoding for proteins involved in cytoskeleton dynamics, we suggest that substrate mechanics can direct PSC specification by influencing cytoskeleton arrangement and intracellular tension. We propose an aberrant activation of YAP-TEAD1 axis alters PSC potency by inhibiting cytoskeleton dynamics, thus paralyzing the changes in shape requested for the acquisition of the given phenotype.
ER  -

PAGLIARI, S., Vladimir VINARSKY, Fabiana MARTINO, A. R. PERESTRELO, J. O. DE LA CRUZ, Guido CALUORI, Jan VRBSKY, P. MOZETIC, A. POMPEIANO, A. ZANCLA, Sri Ranjani GANJI, Petr SKLÁDAL, Dan KYTYR, Zbyněk ZDRÁHAL, G. GRASSI, M. SAMPAOLESI, A. RAINER a G. FORTE. YAP-TEAD1 control of cytoskeleton dynamics and intracellular tension guides human pluripotent stem cell mesoderm specification. \textit{Cell Death and Differentiation}. London: Springer Nature, 2021, roč.~28, č.~4, s.~1193-1207. ISSN~1350-9047. Dostupné z: https://dx.doi.org/10.1038/s41418-020-00643-5.

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