2020
Flower Infusions From Cornus mas and Cornus kousa Inhibit Aldose Reductase Enzyme, Without Any Effects on Lipotoxicity
FORMAN, V., I. SUSANIKOVA, L. KUKUROVA, Emil ŠVAJDLENKA, M. NAGY et. al.Základní údaje
Originální název
Flower Infusions From Cornus mas and Cornus kousa Inhibit Aldose Reductase Enzyme, Without Any Effects on Lipotoxicity
Autoři
FORMAN, V., I. SUSANIKOVA, L. KUKUROVA, Emil ŠVAJDLENKA (203 Česká republika, domácí), M. NAGY a P. MUCAJI
Vydání
Natural Product Communications, THOUSAND OAKS, SAGE PUBLICATIONS INC, 2020, 1934-578X
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30104 Pharmacology and pharmacy
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 0.986
Kód RIV
RIV/00216224:14160/20:00118268
Organizační jednotka
Farmaceutická fakulta
UT WoS
000525438800001
Klíčová slova anglicky
Cornus mas; Cornus kousa; aldose reductase; diabetes mellitus; lipotoxicity; NIH-3T3 cell line
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 25. 2. 2021 10:59, Mgr. Hana Hurtová
Anotace
V originále
Aldose reductase inhibitors are considered to be potential therapeutic agents for chronic diabetic complications. Diabetes mellitus can be accompanied by elevated blood levels of free fatty acids, which can cause lipotoxicity. Herbal extracts and their constituents are promising agents which have the potential for alleviating these complications. Our study was focused on the influence on these effects by flower infusions from Comus mar L. and Cornus kousa F.Buerger ex Hance. Initially, phenolic compounds were quantified in the dried flowers. Next, we studied the ability of flower infusions from both plants to inhibit aldose reductase in vitro, the protective role in the cell model of lipotoxicity, and the cytotoxic action on fibroblast cell line NIH-3T3 by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Both species are rich in phenolics; C. kousa flowers contain slightly higher amounts of phenolic acids (20.8%) and flavonoids (56.1%) than C. mas (20.2%) and (47.4%), respectively. Both extracts showed effective inhibition, expressed as half-maximal inhibitory concentration (IC50) (the concentration of inhibitor required to exhibit 50% inhibition of the enzyme reaction), of aldose reductase in non-toxic low concentrations (IC50 = 3.06 mu g/mL for C. mas and IC50 = 2.49 mu g/mL for C. kousa, respectively). In contrast, these concentrations of both extracts had almost no effects in the lipotoxicity cell model. To our knowledge, this study is the first report on C. mar and C. kousa flowers' aldose reductase inhibitory activity and influence upon lipotoxicity.