Electroanalysis of unnatural base pair content in plasmid DNA
generated in a semi-synthetic organism

J 2020

Electroanalysis of unnatural base pair content in plasmid DNA generated in a semi-synthetic organism

ŠPAČEK, Jan a Miroslav FOJTA

Základní údaje

Originální název

Electroanalysis of unnatural base pair content in plasmid DNA generated in a semi-synthetic organism

Autoři

ŠPAČEK, Jan (203 Česká republika, domácí) a Miroslav FOJTA (203 Česká republika, garant, domácí)

Vydání

Electrochimica Acta, OXFORD, Elsevier, 2020, 0013-4686

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.901

Kód RIV

RIV/00216224:14740/20:00114754

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.electacta.2020.137298

UT WoS

000591743600004

Klíčová slova anglicky

Electrochemistry; Semi-synthetic organism; Unnatural base pair; Catalytic hydrogen evolution; Mercury electrode

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 3. 2021 17:43, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

While standard Watson-Crick base pairing normally involves hydrogen bonds that join size complemen-tary nitrogenous heterocycles, certain unnatural base pairs (UBP) can support replication, transcription, and translation, including these in semi-synthetic organisms (SSO), using size complementarity alone. In this paper we show that the UBPs can be analyzed electrochemically as components of riboor 2'-deoxyribonucleosides, corresponding (2'-deoxy)triphosphates, as well as incorporated into DNA. Here, performance of the electrochemical methods profits from the ability of the unnatural bases 5SICS and TPT3 adsorbed at the surface of a mercury electrode to catalyze hydrogen evolution in acidic media. This allows semi-quantitative detection of single UBP within plasmid DNA generated in a SSO. This electrochemical approach provides new way for direct, fast, and low cost UBP analysis and can supplement or replace currently used methods of UBP detection. (C) 2020 Elsevier Ltd. All rights reserved.

Návaznosti

GBP206/12/G151, projekt VaV

Název: Centrum nových přístupů k bioanalýze a molekulární diagnostice

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

ŠPAČEK, Jan a Miroslav FOJTA. Electroanalysis of unnatural base pair content in plasmid DNA generated in a semi-synthetic organism. Electrochimica Acta. OXFORD: Elsevier, 2020, roč. 364, DEC, s. 137298-137306. ISSN 0013-4686. Dostupné z: https://dx.doi.org/10.1016/j.electacta.2020.137298.

@article{1748058,
   author = {Špaček, Jan and Fojta, Miroslav},
   article_location = {OXFORD},
   article_number = {DEC},
   doi = {http://dx.doi.org/10.1016/j.electacta.2020.137298},
   keywords = {Electrochemistry; Semi-synthetic organism; Unnatural base pair; Catalytic hydrogen evolution; Mercury electrode},
   language = {eng},
   issn = {0013-4686},
   journal = {Electrochimica Acta},
   title = {Electroanalysis of unnatural base pair content in plasmid DNA generated in a semi-synthetic organism},
   url = {https://www.sciencedirect.com/science/article/pii/S0013468620316911?via%3Dihub},
   volume = {364},
   year = {2020}
}

TY  - JOUR
ID  - 1748058
AU  - Špaček, Jan - Fojta, Miroslav
PY  - 2020
TI  - Electroanalysis of unnatural base pair content in plasmid DNA generated in a semi-synthetic organism
JF  - Electrochimica Acta
VL  - 364
IS  - DEC
SP  - 137298
EP  - 137298
PB  - Elsevier
SN  - 00134686
KW  - Electrochemistry
KW  - Semi-synthetic organism
KW  - Unnatural base pair
KW  - Catalytic hydrogen evolution
KW  - Mercury electrode
UR  - https://www.sciencedirect.com/science/article/pii/S0013468620316911?via%3Dihub
N2  - While standard Watson-Crick base pairing normally involves hydrogen bonds that join size complemen-tary nitrogenous heterocycles, certain unnatural base pairs (UBP) can support replication, transcription, and translation, including these in semi-synthetic organisms (SSO), using size complementarity alone. In this paper we show that the UBPs can be analyzed electrochemically as components of riboor 2'-deoxyribonucleosides, corresponding (2'-deoxy)triphosphates, as well as incorporated into DNA. Here, performance of the electrochemical methods profits from the ability of the unnatural bases 5SICS and TPT3 adsorbed at the surface of a mercury electrode to catalyze hydrogen evolution in acidic media. This allows semi-quantitative detection of single UBP within plasmid DNA generated in a SSO. This electrochemical approach provides new way for direct, fast, and low cost UBP analysis and can supplement or replace currently used methods of UBP detection. (C) 2020 Elsevier Ltd. All rights reserved.
ER  -

ŠPAČEK, Jan a Miroslav FOJTA. Electroanalysis of unnatural base pair content in plasmid DNA generated in a semi-synthetic organism. \textit{Electrochimica Acta}. OXFORD: Elsevier, 2020, roč.~364, DEC, s.~137298-137306. ISSN~0013-4686. Dostupné z: https://dx.doi.org/10.1016/j.electacta.2020.137298.

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