Další formáty:
BibTeX
LaTeX
RIS
@article{1748118,
author = {Kamentseva, R. and Kosheverova, V. and Kharchenko, M. and Zlobina, Maria and Salova, A. and Belyaeva, T. and Nikolsky, N. and Kornilova, E.},
article_location = {San Francisco},
article_number = {5},
doi = {http://dx.doi.org/10.1371/journal.pone.0232532},
keywords = {EPIDERMAL-GROWTH-FACTOR; EGF RECEPTOR ENDOCYTOSIS; RAB5; EEA1; MEMBRANE; MATURATION; PROTEIN; TRANSFERRIN; RECRUITMENT; CONVERSION},
language = {eng},
issn = {1932-6203},
journal = {Plos one},
title = {Functional cycle of EEA1-positive early endosome: Direct evidence for pre-existing compartment of degradative pathway},
url = {https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0232532},
volume = {15},
year = {2020}
}
TY - JOUR
ID - 1748118
AU - Kamentseva, R. - Kosheverova, V. - Kharchenko, M. - Zlobina, Maria - Salova, A. - Belyaeva, T. - Nikolsky, N. - Kornilova, E.
PY - 2020
TI - Functional cycle of EEA1-positive early endosome: Direct evidence for pre-existing compartment of degradative pathway
JF - Plos one
VL - 15
IS - 5
SP - 0232532
EP - 0232532
PB - Public Library of Science
SN - 19326203
KW - EPIDERMAL-GROWTH-FACTOR
KW - EGF RECEPTOR ENDOCYTOSIS
KW - RAB5
KW - EEA1
KW - MEMBRANE
KW - MATURATION
KW - PROTEIN
KW - TRANSFERRIN
KW - RECRUITMENT
KW - CONVERSION
UR - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0232532
N2 - Early endosomes, regarded as the main sorting station on endocytic pathway, are characterized by high frequency of homotypic fusions mediated by tethering protein EEA1. Despite intensive investigations, biogenesis of endosomes, boundaries between early and late endosomes, and process of cargo transition though them remain obscure. Here, using EGF/EGFR endocytosis as a model and confocal microscopy of fixed and live cells, we provide evidence favoring EEA1-vesicles being pre-existed vesicular compartment, that maintains its resident proteins' level and is sensitive to biosynthetic, but not endocytic pathway disturbance. EEA1-vesicles directly fuse with incoming EGF/EGFR-vesicles into hybrid endosomes with separated EEA1- and EGFR-domains, thus providing a platform for rapid achievement of an excess of surface-derived membrane that is used to form intraluminal vesicles (ILVs). Thus, multivesicular structures colocalized with EEA1 are still early endosomes. "EEA1-cycle" ends by exclusion of EGFR-containing domains with ILVs inside that turns into MVE and restoration of initial EEA1-vesicles population.
ER -
KAMENTSEVA, R., V. KOSHEVEROVA, M. KHARCHENKO, Maria ZLOBINA, A. SALOVA, T. BELYAEVA, N. NIKOLSKY a E. KORNILOVA. Functional cycle of EEA1-positive early endosome: Direct evidence for pre-existing compartment of degradative pathway. \textit{Plos one}. San Francisco: Public Library of Science, 2020, roč.~15, č.~5, s.~0232532-232557. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0232532.
|
