Imaging plant germline differentiation within Arabidopsis
flowers by light sheet microscopy

J 2020

Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy

VALUCHOVÁ, Soňa, Pavlína MIKULKOVÁ, Jana PEČINKOVÁ, J. KLIMOVA, M. KRUMNIKL et. al.

Základní údaje

Originální název

Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy

Autoři

VALUCHOVÁ, Soňa (703 Slovensko, domácí), Pavlína MIKULKOVÁ (203 Česká republika, domácí), Jana PEČINKOVÁ (203 Česká republika, domácí), J. KLIMOVA, M. KRUMNIKL, P. BAINAR, S. HECKMANN, P. TOMANCAK a Karel ŘÍHA (203 Česká republika, garant, domácí)

Vydání

elife, CAMBRIDGE, ELIFE SCIENCES PUBLICATIONS LTD, 2020, 2050-084X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 8.140

Kód RIV

RIV/00216224:14740/20:00118319

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.7554/eLife.52546

UT WoS

000514104400001

Klíčová slova anglicky

OPTICAL MANIPULATION; MOLECULAR-BIOLOGY; MEIOTIC PROPHASE; THALIANA; MEIOSIS; VISUALIZATION; SEGREGATION; EVOLUTION; PROGRAM; TAPETUM

Štítky

CF CELLIM, CF PLANT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 10. 2024 12:50, Mgr. Adéla Pešková

Anotace

V originále

In higher plants, germline differentiation occurs during a relatively short period within developing flowers. Understanding of the mechanisms that govern germline differentiation lags behind other plant developmental processes. This is largely because the germline is restricted to relatively few cells buried deep within floral tissues, which makes them difficult to study. To overcome this limitation, we have developed a methodology for live imaging of the germ cell lineage within floral organs of Arabidopsis using light sheet fluorescence microscopy. We have established reporter lines, cultivation conditions, and imaging protocols for high-resolution microscopy of developing flowers continuously for up to several days. We used multiview imagining to reconstruct a three-dimensional model of a flower at subcellular resolution. We demonstrate the power of this approach by capturing male and female meiosis, asymmetric pollen division, movement of meiotic chromosomes, and unusual restitution mitosis in tapetum cells. This method will enable new avenues of research into plant sexual reproduction.

Návaznosti

EF15_003/0000479, projekt VaV

Název: Regulace rostlinné meiózy

90062, velká výzkumná infrastruktura

Název: Czech-BioImaging

Citovat

VALUCHOVÁ, Soňa, Pavlína MIKULKOVÁ, Jana PEČINKOVÁ, J. KLIMOVA, M. KRUMNIKL, P. BAINAR, S. HECKMANN, P. TOMANCAK a Karel ŘÍHA. Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy. elife. CAMBRIDGE: ELIFE SCIENCES PUBLICATIONS LTD, 2020, roč. 9, FEB, s. 52546-52564. ISSN 2050-084X. Dostupné z: https://dx.doi.org/10.7554/eLife.52546.

@article{1748856,
   author = {Valuchová, Soňa and Mikulková, Pavlína and Pečinková, Jana and Klimova, J. and Krumnikl, M. and Bainar, P. and Heckmann, S. and Tomancak, P. and Říha, Karel},
   article_location = {CAMBRIDGE},
   article_number = {FEB},
   doi = {http://dx.doi.org/10.7554/eLife.52546},
   keywords = {OPTICAL MANIPULATION; MOLECULAR-BIOLOGY; MEIOTIC PROPHASE; THALIANA; MEIOSIS; VISUALIZATION; SEGREGATION; EVOLUTION; PROGRAM; TAPETUM},
   language = {eng},
   issn = {2050-084X},
   journal = {elife},
   title = {Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy},
   url = {https://elifesciences.org/articles/52546},
   volume = {9},
   year = {2020}
}

TY  - JOUR
ID  - 1748856
AU  - Valuchová, Soňa - Mikulková, Pavlína - Pečinková, Jana - Klimova, J. - Krumnikl, M. - Bainar, P. - Heckmann, S. - Tomancak, P. - Říha, Karel
PY  - 2020
TI  - Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy
JF  - elife
VL  - 9
IS  - FEB
SP  - 52546
EP  - 52546
PB  - ELIFE SCIENCES PUBLICATIONS LTD
SN  - 2050084X
KW  - OPTICAL MANIPULATION
KW  - MOLECULAR-BIOLOGY
KW  - MEIOTIC PROPHASE
KW  - THALIANA
KW  - MEIOSIS
KW  - VISUALIZATION
KW  - SEGREGATION
KW  - EVOLUTION
KW  - PROGRAM
KW  - TAPETUM
UR  - https://elifesciences.org/articles/52546
N2  - In higher plants, germline differentiation occurs during a relatively short period within developing flowers. Understanding of the mechanisms that govern germline differentiation lags behind other plant developmental processes. This is largely because the germline is restricted to relatively few cells buried deep within floral tissues, which makes them difficult to study. To overcome this limitation, we have developed a methodology for live imaging of the germ cell lineage within floral organs of Arabidopsis using light sheet fluorescence microscopy. We have established reporter lines, cultivation conditions, and imaging protocols for high-resolution microscopy of developing flowers continuously for up to several days. We used multiview imagining to reconstruct a three-dimensional model of a flower at subcellular resolution. We demonstrate the power of this approach by capturing male and female meiosis, asymmetric pollen division, movement of meiotic chromosomes, and unusual restitution mitosis in tapetum cells. This method will enable new avenues of research into plant sexual reproduction.
ER  -

VALUCHOVÁ, Soňa, Pavlína MIKULKOVÁ, Jana PEČINKOVÁ, J. KLIMOVA, M. KRUMNIKL, P. BAINAR, S. HECKMANN, P. TOMANCAK a Karel ŘÍHA. Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy. \textit{elife}. CAMBRIDGE: ELIFE SCIENCES PUBLICATIONS LTD, 2020, roč.~9, FEB, s.~52546-52564. ISSN~2050-084X. Dostupné z: https://dx.doi.org/10.7554/eLife.52546.

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