Podrobný výpis o publikaci

PLOCHBERGER, B., T. SYCH, F. WEBER, Jiří NOVÁČEK, M. AXMANN, H. STANGL a E. SEZGIN. Lipoprotein Particles Interact with Membranes and Transfer Their Cargo without Receptors. Biochemistry. Washington: American Chemical Society, 2020, roč. 59, č. 45, s. 4421-4428. ISSN 0006-2960. Dostupné z: https://dx.doi.org/10.1021/acs.biochem.0c00748.

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Základní údaje
Originální název	Lipoprotein Particles Interact with Membranes and Transfer Their Cargo without Receptors
Autoři	PLOCHBERGER, B., T. SYCH, F. WEBER, Jiří NOVÁČEK (203 Česká republika, garant, domácí), M. AXMANN, H. STANGL a E. SEZGIN.
Vydání	Biochemistry, Washington, American Chemical Society, 2020, 0006-2960.

Další údaje
Originální jazyk	angličtina
Typ výsledku	Článek v odborném periodiku
Obor	10608 Biochemistry and molecular biology
Stát vydavatele	Spojené státy
Utajení	není předmětem státního či obchodního tajemství
WWW	URL
Impakt faktor	Impact factor: 3.162
Kód RIV	RIV/00216224:14740/20:00118331
Organizační jednotka	Středoevropský technologický institut
Doi	http://dx.doi.org/10.1021/acs.biochem.0c00748
UT WoS	000592835200010
Klíčová slova anglicky	CHOLESTEROL
Štítky	rivok
Příznaky	Mezinárodní význam, Recenzováno
Změnil	Změnila: Mgr. Pavla Foltynová, Ph.D., učo 106624. Změněno: 4. 3. 2021 16:37.

Anotace
Lipid transfer from lipoprotein particles to cells is essential for lipid homeostasis. High-density lipoprotein (HDL) particles are mainly captured by cell membrane-associated scavenger receptor class B type 1 (SR-B1) from the bloodstream, while low-density and very-low-density lipoprotein (LDL and VLDL, respectively) particles are mostly taken up by receptor-mediated endocytosis. However, the role of the target lipid membrane itself in the transfer process has been largely neglected so far. Here, we study how lipoprotein particles (HDL, LDL, and VLDL) interact with synthetic lipid bilayers and cell-derived membranes and transfer their cargo subsequently. Employing cryoelectron microscopy, spectral imaging, and fluorescence (cross correlation spectroscopy allowed us to observe integration of all major types of lipoprotein particles into the membrane and delivery of their cargo in a receptor-independent manner. Importantly, the biophysical properties of the target cell membranes change upon delivery of cargo. The concept of receptor-independent interaction of lipoprotein particles with membranes helps us to better understand lipoprotein particle biology and can be exploited for novel treatments of dyslipidemia diseases.

Anotace

Lipid transfer from lipoprotein particles to cells is essential for lipid homeostasis. High-density lipoprotein (HDL) particles are mainly captured by cell membrane-associated scavenger receptor class B type 1 (SR-B1) from the bloodstream, while low-density and very-low-density lipoprotein (LDL and VLDL, respectively) particles are mostly taken up by receptor-mediated endocytosis. However, the role of the target lipid membrane itself in the transfer process has been largely neglected so far. Here, we study how lipoprotein particles (HDL, LDL, and VLDL) interact with synthetic lipid bilayers and cell-derived membranes and transfer their cargo subsequently. Employing cryoelectron microscopy, spectral imaging, and fluorescence (cross correlation spectroscopy allowed us to observe integration of all major types of lipoprotein particles into the membrane and delivery of their cargo in a receptor-independent manner. Importantly, the biophysical properties of the target cell membranes change upon delivery of cargo. The concept of receptor-independent interaction of lipoprotein particles with membranes helps us to better understand lipoprotein particle biology and can be exploited for novel treatments of dyslipidemia diseases.