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@article{1749696,
author = {Bezdekova, J. and Vlcnovska, M. and Zemankova, K. and Bacova, R. and Kolackova, M. and Lednicky, T. and Přibyl, Jan and Richtera, L. and Vanickova, L. and Adam, V. and Vaculovicova, M.},
article_location = {New York},
article_number = {6},
doi = {http://dx.doi.org/10.3168/jds.2019-17367},
keywords = {biochanin A; genistein; dopamine; milk},
language = {eng},
issn = {0022-0302},
journal = {Journal of Dairy Science},
title = {Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk},
url = {https://www.sciencedirect.com/science/article/abs/pii/S0022030220302666?via%3Dihub},
volume = {103},
year = {2020}
}
TY - JOUR
ID - 1749696
AU - Bezdekova, J. - Vlcnovska, M. - Zemankova, K. - Bacova, R. - Kolackova, M. - Lednicky, T. - Přibyl, Jan - Richtera, L. - Vanickova, L. - Adam, V. - Vaculovicova, M.
PY - 2020
TI - Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk
JF - Journal of Dairy Science
VL - 103
IS - 6
SP - 4941-4950
EP - 4941-4950
PB - Elsevier Science Inc.
SN - 00220302
KW - biochanin A
KW - genistein
KW - dopamine
KW - milk
UR - https://www.sciencedirect.com/science/article/abs/pii/S0022030220302666?via%3Dihub
N2 - Dairy cow feed contains, among other ingredients, soybeans, legumes, and clover, plants that are rich in phytoestrogens. Several publications have reported a positive influence of phytoestrogens on human health; however, several unfavorable effects have also been reported. In this work, a simple, selective, and ecofriendly method of phytoestrogen isolation based on the technique of noncovalent molecular imprinting was developed. Genistein was used as a template, and dopamine was chosen as a functional monomer. A layer of molecularly imprinted polymers was created in a microtitration well plate. The binding capability and selective properties of obtained molecularly imprinted polymers were investigated. The imprinted polymers exhibited higher binding affinity toward chosen phytoestrogen than did the nonimprinted polymers. A selectivity factor of 6.94 was calculated, confirming satisfactory selectivity of the polymeric layer. The applicability of the proposed sensing method was tested by isolation of genistein from a real sample of bovine milk and combined with micellar electrokinetic capillary chromatography with UV-visible detection.
ER -
BEZDEKOVA, J., M. VLCNOVSKA, K. ZEMANKOVA, R. BACOVA, M. KOLACKOVA, T. LEDNICKY, Jan PŘIBYL, L. RICHTERA, L. VANICKOVA, V. ADAM a M. VACULOVICOVA. Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk. \textit{Journal of Dairy Science}. New York: Elsevier Science Inc., 2020, roč.~103, č.~6, s.~4941-4950. ISSN~0022-0302. Dostupné z: https://dx.doi.org/10.3168/jds.2019-17367.
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