Nonsense-Mediated RNA Decay Factor UPF1 Is Critical for
Posttranscriptional and Translational Gene Regulation in
Arabidopsis

J 2020

Nonsense-Mediated RNA Decay Factor UPF1 Is Critical for Posttranscriptional and Translational Gene Regulation in Arabidopsis

RAXWAL, Vivek Kumar, C.G. SIMPSON, J. GLOGGNITZER, J.C. ENTINZE, W.B. GUO et. al.

Základní údaje

Originální název

Nonsense-Mediated RNA Decay Factor UPF1 Is Critical for Posttranscriptional and Translational Gene Regulation in Arabidopsis

Autoři

RAXWAL, Vivek Kumar (356 Indie, domácí), C.G. SIMPSON, J. GLOGGNITZER, J.C. ENTINZE, W.B. GUO, R.X. ZHANG, J.W.S. BROWN a Karel ŘÍHA (203 Česká republika, garant, domácí)

Vydání

Plant Cell, ROCKVILLE, AMER SOC PLANT BIOLOGISTS, 2020, 1040-4651

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 11.277

Kód RIV

RIV/00216224:14740/20:00114761

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1105/tpc.20.00244

UT WoS

000578504100015

Klíčová slova anglicky

MESSENGER-RNA; TRIGGERED IMMUNITY; COMPLEX; NMD; IDENTIFICATION; TERMINATION; EXPRESSION; TRANSCRIPTOME; SURVEILLANCE; DEGRADATION

Štítky

CF PLANT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 3. 2021 11:50, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Nonsense-mediated RNA decay (NMD) is an RNA control mechanism that has also been implicated in the broader regulation of gene expression. Nevertheless, a role for NMD in genome regulation has not yet been fully assessed, partially because NMD inactivation is lethal in many organisms. Here, we performed an in-depth comparative analysis of Arabidopsis (Arabidopsis thaliana) mutants lacking the NMD-related proteins UPF3, UPF1, and SMG7. We found different impacts of these proteins on NMD and the Arabidopsis transcriptome, with UPF1 having the biggest effect. Transcriptome assembly in UPF1-null plants revealed genome-wide changes in alternative splicing, suggesting that UPF1 functions in splicing. The inactivation of UPF1 led to translational repression, as manifested by a global shift in mRNAs from polysomes to monosomes and the downregulation of genes involved in translation and ribosome biogenesis. Despite these global changes, NMD targets and mRNAs expressed at low levels with short half-lives were enriched in the polysomes ofupf1mutants, indicating that UPF1/NMD suppresses the translation of aberrant RNAs. Particularly striking was an increase in the translation of TIR domain-containing, nucleotide binding, leucine-rich repeat (TNL) immune receptors. The regulation of TNLs via UPF1/NMD-mediated mRNA stability and translational derepression offers a dynamic mechanism for the rapid activation of TNLs in response to pathogen attack. The RNA helicase and ATPase UPF1 affects transcriptome homeostasis and gene expression through nonsense-mediated RNA decay, splicing, and translation in Arabidopsis.

Návaznosti

EF15_003/0000479, projekt VaV

Název: Regulace rostlinné meiózy

GA16-18578S, projekt VaV

Název: Funkce UPF1 helikázy v procesech odehrávajících se v buněčném jádře

Investor: Grantová agentura ČR, Funkce UPF1 helikázy v procesech odehrávajících se v buněčném jádře

Citovat

RAXWAL, Vivek Kumar, C.G. SIMPSON, J. GLOGGNITZER, J.C. ENTINZE, W.B. GUO, R.X. ZHANG, J.W.S. BROWN a Karel ŘÍHA. Nonsense-Mediated RNA Decay Factor UPF1 Is Critical for Posttranscriptional and Translational Gene Regulation in Arabidopsis. Plant Cell. ROCKVILLE: AMER SOC PLANT BIOLOGISTS, 2020, roč. 32, č. 9, s. 2725-2741. ISSN 1040-4651. Dostupné z: https://dx.doi.org/10.1105/tpc.20.00244.

@article{1749838,
   author = {Raxwal, Vivek Kumar and Simpson, C.G. and Gloggnitzer, J. and Entinze, J.C. and Guo, W.B. and Zhang, R.X. and Brown, J.W.S. and Říha, Karel},
   article_location = {ROCKVILLE},
   article_number = {9},
   doi = {http://dx.doi.org/10.1105/tpc.20.00244},
   keywords = {MESSENGER-RNA; TRIGGERED IMMUNITY; COMPLEX; NMD; IDENTIFICATION; TERMINATION; EXPRESSION; TRANSCRIPTOME; SURVEILLANCE; DEGRADATION},
   language = {eng},
   issn = {1040-4651},
   journal = {Plant Cell},
   title = {Nonsense-Mediated RNA Decay Factor UPF1 Is Critical for Posttranscriptional and Translational Gene Regulation in Arabidopsis},
   url = {https://academic.oup.com/plcell/article/32/9/2725/6115658},
   volume = {32},
   year = {2020}
}

TY  - JOUR
ID  - 1749838
AU  - Raxwal, Vivek Kumar - Simpson, C.G. - Gloggnitzer, J. - Entinze, J.C. - Guo, W.B. - Zhang, R.X. - Brown, J.W.S. - Říha, Karel
PY  - 2020
TI  - Nonsense-Mediated RNA Decay Factor UPF1 Is Critical for Posttranscriptional and Translational Gene Regulation in Arabidopsis
JF  - Plant Cell
VL  - 32
IS  - 9
SP  - 2725-2741
EP  - 2725-2741
PB  - AMER SOC PLANT BIOLOGISTS
SN  - 10404651
KW  - MESSENGER-RNA
KW  - TRIGGERED IMMUNITY
KW  - COMPLEX
KW  - NMD
KW  - IDENTIFICATION
KW  - TERMINATION
KW  - EXPRESSION
KW  - TRANSCRIPTOME
KW  - SURVEILLANCE
KW  - DEGRADATION
UR  - https://academic.oup.com/plcell/article/32/9/2725/6115658
N2  - Nonsense-mediated RNA decay (NMD) is an RNA control mechanism that has also been implicated in the broader regulation of gene expression. Nevertheless, a role for NMD in genome regulation has not yet been fully assessed, partially because NMD inactivation is lethal in many organisms. Here, we performed an in-depth comparative analysis of Arabidopsis (Arabidopsis thaliana) mutants lacking the NMD-related proteins UPF3, UPF1, and SMG7. We found different impacts of these proteins on NMD and the Arabidopsis transcriptome, with UPF1 having the biggest effect. Transcriptome assembly in UPF1-null plants revealed genome-wide changes in alternative splicing, suggesting that UPF1 functions in splicing. The inactivation of UPF1 led to translational repression, as manifested by a global shift in mRNAs from polysomes to monosomes and the downregulation of genes involved in translation and ribosome biogenesis. Despite these global changes, NMD targets and mRNAs expressed at low levels with short half-lives were enriched in the polysomes ofupf1mutants, indicating that UPF1/NMD suppresses the translation of aberrant RNAs. Particularly striking was an increase in the translation of TIR domain-containing, nucleotide binding, leucine-rich repeat (TNL) immune receptors. The regulation of TNLs via UPF1/NMD-mediated mRNA stability and translational derepression offers a dynamic mechanism for the rapid activation of TNLs in response to pathogen attack. The RNA helicase and ATPase UPF1 affects transcriptome homeostasis and gene expression through nonsense-mediated RNA decay, splicing, and translation in Arabidopsis.
ER  -

RAXWAL, Vivek Kumar, C.G. SIMPSON, J. GLOGGNITZER, J.C. ENTINZE, W.B. GUO, R.X. ZHANG, J.W.S. BROWN a Karel ŘÍHA. Nonsense-Mediated RNA Decay Factor UPF1 Is Critical for Posttranscriptional and Translational Gene Regulation in Arabidopsis. \textit{Plant Cell}. ROCKVILLE: AMER SOC PLANT BIOLOGISTS, 2020, roč.~32, č.~9, s.~2725-2741. ISSN~1040-4651. Dostupné z: https://dx.doi.org/10.1105/tpc.20.00244.

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