Recurrent gene mutations detected in canine mast cell tumours
by next generation sequencing

J 2020

Recurrent gene mutations detected in canine mast cell tumours by next generation sequencing

VOZDOVA, M., S. KUBICKOVA, Karol PÁL, J. FROHLICH, P. FICTUM et. al.

Základní údaje

Originální název

Recurrent gene mutations detected in canine mast cell tumours by next generation sequencing

Autoři

VOZDOVA, M., S. KUBICKOVA, Karol PÁL (703 Slovensko, garant, domácí), J. FROHLICH, P. FICTUM a J. RUBES

Vydání

VETERINARY AND COMPARATIVE ONCOLOGY, HOBOKEN, WILEY, 2020, 1476-5810

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40301 Veterinary science

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.613

Kód RIV

RIV/00216224:14740/20:00118346

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1111/vco.12572

UT WoS

000590128500007

Klíčová slova anglicky

cancer; dog; GNB1; KIT; mast cell tumour; mutation; next generation sequencing; whole exome sequencing

Štítky

CF GEN, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 10. 2024 09:21, Ing. Martina Blahová

Anotace

V originále

Genetic causes of canine mast cell tumours (MCTs), except for mutations in the KIT gene detected in some MCTs, are generally unknown. We used whole exome sequencing to reveal mutation spectra in canine MCTs. We detected somatic mutations in 87 genes including 10 genes recognized as human cancer drivers. Besides KIT, 14 other genes were recurrently mutated. Subsequently, we performed next generation sequencing of a panel of 50 selected genes in additional MCT samples. In this group, the most frequently altered gene was GNB1 showing a recurrent dinucleotide substitution at position of Gly116 in 30% of the MCT samples (n = 6/20) and Ile80 substitution accompanied by a splice region mutation in one case. We extended the study by analysis of the above mentioned GNB1 regions in additional MCT samples by Sanger sequencing, and assessed the overall prevalence of GNB1 mutations to 17.3% (n = 14/81), which is similar to the prevalence of KIT alterations. Our results indicate that GNB1 mutations are probably involved in canine MCT pathogenesis in both cutaneous and subcutaneous MCT cases. As opposed to KIT alterations, the presence of GNB1 mutations did not negatively affect survival times, and our data even showed a trend towards positive prognosis. If our results are confirmed in a larger number of MCTs, an extension of molecular testing of canine MCTs by GNB1 analysis would help to refine the molecular stratification of MCTs, and become useful for targeted treatment strategies.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

90091, velká výzkumná infrastruktura

Název: NCMG

Citovat

VOZDOVA, M., S. KUBICKOVA, Karol PÁL, J. FROHLICH, P. FICTUM a J. RUBES. Recurrent gene mutations detected in canine mast cell tumours by next generation sequencing. VETERINARY AND COMPARATIVE ONCOLOGY. HOBOKEN: WILEY, 2020, roč. 18, č. 4, s. 509-518. ISSN 1476-5810. Dostupné z: https://dx.doi.org/10.1111/vco.12572.

@article{1750438,
   author = {Vozdova, M. and Kubickova, S. and Pál, Karol and Frohlich, J. and Fictum, P. and Rubes, J.},
   article_location = {HOBOKEN},
   article_number = {4},
   doi = {http://dx.doi.org/10.1111/vco.12572},
   keywords = {cancer; dog; GNB1; KIT; mast cell tumour; mutation; next generation sequencing; whole exome sequencing},
   language = {eng},
   issn = {1476-5810},
   journal = {VETERINARY AND COMPARATIVE ONCOLOGY},
   title = {Recurrent gene mutations detected in canine mast cell tumours by next generation sequencing},
   url = {https://onlinelibrary.wiley.com/doi/10.1111/vco.12572},
   volume = {18},
   year = {2020}
}

TY  - JOUR
ID  - 1750438
AU  - Vozdova, M. - Kubickova, S. - Pál, Karol - Frohlich, J. - Fictum, P. - Rubes, J.
PY  - 2020
TI  - Recurrent gene mutations detected in canine mast cell tumours by next generation sequencing
JF  - VETERINARY AND COMPARATIVE ONCOLOGY
VL  - 18
IS  - 4
SP  - 509-518
EP  - 509-518
PB  - WILEY
SN  - 14765810
KW  - cancer
KW  - dog
KW  - GNB1
KW  - KIT
KW  - mast cell tumour
KW  - mutation
KW  - next generation sequencing
KW  - whole exome sequencing
UR  - https://onlinelibrary.wiley.com/doi/10.1111/vco.12572
N2  - Genetic causes of canine mast cell tumours (MCTs), except for mutations in the KIT gene detected in some MCTs, are generally unknown. We used whole exome sequencing to reveal mutation spectra in canine MCTs. We detected somatic mutations in 87 genes including 10 genes recognized as human cancer drivers. Besides KIT, 14 other genes were recurrently mutated. Subsequently, we performed next generation sequencing of a panel of 50 selected genes in additional MCT samples. In this group, the most frequently altered gene was GNB1 showing a recurrent dinucleotide substitution at position of Gly116 in 30% of the MCT samples (n = 6/20) and Ile80 substitution accompanied by a splice region mutation in one case. We extended the study by analysis of the above mentioned GNB1 regions in additional MCT samples by Sanger sequencing, and assessed the overall prevalence of GNB1 mutations to 17.3% (n = 14/81), which is similar to the prevalence of KIT alterations. Our results indicate that GNB1 mutations are probably involved in canine MCT pathogenesis in both cutaneous and subcutaneous MCT cases. As opposed to KIT alterations, the presence of GNB1 mutations did not negatively affect survival times, and our data even showed a trend towards positive prognosis. If our results are confirmed in a larger number of MCTs, an extension of molecular testing of canine MCTs by GNB1 analysis would help to refine the molecular stratification of MCTs, and become useful for targeted treatment strategies.
ER  -

VOZDOVA, M., S. KUBICKOVA, Karol PÁL, J. FROHLICH, P. FICTUM a J. RUBES. Recurrent gene mutations detected in canine mast cell tumours by next generation sequencing. \textit{VETERINARY AND COMPARATIVE ONCOLOGY}. HOBOKEN: WILEY, 2020, roč.~18, č.~4, s.~509-518. ISSN~1476-5810. Dostupné z: https://dx.doi.org/10.1111/vco.12572.

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