The Structural Integrity of the Model Lipid Membrane during
Induced Lipid Peroxidation: The Role of Flavonols in the
Inhibition of Lipid Peroxidation

J 2020

The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation

SADZAK, A., J. MRAVLJAK, N. MALTAR-STRMECKI, Z. ARSOV, G. BARANOVIC et. al.

Basic information

Original name

The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation

Authors

SADZAK, A., J. MRAVLJAK, N. MALTAR-STRMECKI, Z. ARSOV, G. BARANOVIC, I. ERCEG, M. KRIECHBAUM, V. STRASSER, Jan PŘIBYL (203 Czech Republic, guarantor, belonging to the institution) and S. SEGOTA

Edition

Antioxidants, Basel, MDPI, 2020, 2076-3921

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.312

RIV identification code

RIV/00216224:14740/20:00118363

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3390/antiox9050430

UT WoS

000539284200073

Keywords in English

bilayer thickness; elasticity; flavonols; fluidity; lipid peroxidation; myricetin; myricitrin; quercetin

Abstract

V originále

The structural integrity, elasticity, and fluidity of lipid membranes are critical for cellular activities such as communication between cells, exocytosis, and endocytosis. Unsaturated lipids, the main components of biological membranes, are particularly susceptible to the oxidative attack of reactive oxygen species. The peroxidation of unsaturated lipids, in our case 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), induces the structural reorganization of the membrane. We have employed a multi-technique approach to analyze typical properties of lipid bilayers, i.e., roughness, thickness, elasticity, and fluidity. We compared the alteration of the membrane properties upon initiated lipid peroxidation and examined the ability of flavonols, namely quercetin (QUE), myricetin (MCE), and myricitrin (MCI) at different molar fractions, to inhibit this change. Using Mass Spectrometry (MS) and Fourier Transform Infrared Spectroscopy (FTIR), we identified various carbonyl products and examined the extent of the reaction. From Atomic Force Microscopy (AFM), Force Spectroscopy (FS), Small Angle X-Ray Scattering (SAXS), and Electron Paramagnetic Resonance (EPR) experiments, we concluded that the membranes with inserted flavonols exhibit resistance against the structural changes induced by the oxidative attack, which is a finding with multiple biological implications. Our approach reveals the interplay between the flavonol molecular structure and the crucial membrane properties under oxidative attack and provides insight into the pathophysiology of cellular oxidative injury.

Links

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

90127, large research infrastructures

Name: CIISB II

Citovat

SADZAK, A., J. MRAVLJAK, N. MALTAR-STRMECKI, Z. ARSOV, G. BARANOVIC, I. ERCEG, M. KRIECHBAUM, V. STRASSER, Jan PŘIBYL and S. SEGOTA. The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation. Antioxidants. Basel: MDPI, 2020, vol. 9, No 5, p. 430-457. ISSN 2076-3921. Available from: https://dx.doi.org/10.3390/antiox9050430.

@article{1751057,
   author = {Sadzak, A. and Mravljak, J. and MaltarandStrmecki, N. and Arsov, Z. and Baranovic, G. and Erceg, I. and Kriechbaum, M. and Strasser, V. and Přibyl, Jan and Segota, S.},
   article_location = {Basel},
   article_number = {5},
   doi = {http://dx.doi.org/10.3390/antiox9050430},
   keywords = {bilayer thickness; elasticity; flavonols; fluidity; lipid peroxidation; myricetin; myricitrin; quercetin},
   language = {eng},
   issn = {2076-3921},
   journal = {Antioxidants},
   title = {The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation},
   url = {https://www.mdpi.com/2076-3921/9/5/430},
   volume = {9},
   year = {2020}
}

TY  - JOUR
ID  - 1751057
AU  - Sadzak, A. - Mravljak, J. - Maltar-Strmecki, N. - Arsov, Z. - Baranovic, G. - Erceg, I. - Kriechbaum, M. - Strasser, V. - Přibyl, Jan - Segota, S.
PY  - 2020
TI  - The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation
JF  - Antioxidants
VL  - 9
IS  - 5
SP  - 430
EP  - 430
PB  - MDPI
SN  - 20763921
KW  - bilayer thickness
KW  - elasticity
KW  - flavonols
KW  - fluidity
KW  - lipid peroxidation
KW  - myricetin
KW  - myricitrin
KW  - quercetin
UR  - https://www.mdpi.com/2076-3921/9/5/430
N2  - The structural integrity, elasticity, and fluidity of lipid membranes are critical for cellular activities such as communication between cells, exocytosis, and endocytosis. Unsaturated lipids, the main components of biological membranes, are particularly susceptible to the oxidative attack of reactive oxygen species. The peroxidation of unsaturated lipids, in our case 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), induces the structural reorganization of the membrane. We have employed a multi-technique approach to analyze typical properties of lipid bilayers, i.e., roughness, thickness, elasticity, and fluidity. We compared the alteration of the membrane properties upon initiated lipid peroxidation and examined the ability of flavonols, namely quercetin (QUE), myricetin (MCE), and myricitrin (MCI) at different molar fractions, to inhibit this change. Using Mass Spectrometry (MS) and Fourier Transform Infrared Spectroscopy (FTIR), we identified various carbonyl products and examined the extent of the reaction. From Atomic Force Microscopy (AFM), Force Spectroscopy (FS), Small Angle X-Ray Scattering (SAXS), and Electron Paramagnetic Resonance (EPR) experiments, we concluded that the membranes with inserted flavonols exhibit resistance against the structural changes induced by the oxidative attack, which is a finding with multiple biological implications. Our approach reveals the interplay between the flavonol molecular structure and the crucial membrane properties under oxidative attack and provides insight into the pathophysiology of cellular oxidative injury.
ER  -

SADZAK, A., J. MRAVLJAK, N. MALTAR-STRMECKI, Z. ARSOV, G. BARANOVIC, I. ERCEG, M. KRIECHBAUM, V. STRASSER, Jan PŘIBYL and S. SEGOTA. The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation. \textit{Antioxidants}. Basel: MDPI, 2020, vol.~9, No~5, p.~430-457. ISSN~2076-3921. Available from: https://dx.doi.org/10.3390/antiox9050430.

Detailed Information on Publication Record