KLIMOVIČ, Šimon, Jan PŘIBYL, ROTREKL, Šárka JELÍNKOVÁ a H. VICAROVA. COVALENTLY CROSS-LINKED HYALURONIC ACID/BSA/GELATINE HYDROGELS AS BETTER SURFACE FOR CELL CULTURE. Online. In Nanocon 2020. 2020. vyd. Brno: TANGER Ltd., 2020, s. 1-7. ISSN 2694-930X. Dostupné z: https://dx.doi.org/10.37904/nanocon.2020.3739.
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Základní údaje
Originální název COVALENTLY CROSS-LINKED HYALURONIC ACID/BSA/GELATINE HYDROGELS AS BETTER SURFACE FOR CELL CULTURE
Autoři KLIMOVIČ, Šimon, Jan PŘIBYL, ROTREKL, Šárka JELÍNKOVÁ a H. VICAROVA.
Vydání 2020. vyd. Brno, Nanocon 2020, od s. 1-7, 7 s. 2020.
Nakladatel TANGER Ltd.
Další údaje
Originální jazyk angličtina
Typ výsledku Stať ve sborníku
Obor 10601 Cell biology
Stát vydavatele Česká republika
Utajení není předmětem státního či obchodního tajemství
Forma vydání elektronická verze "online"
WWW URL
Organizační jednotka Středoevropský technologický institut
ISSN 2694-930X
Doi http://dx.doi.org/10.37904/nanocon.2020.3739
Klíčová slova anglicky AFM; Hydrogel; Actin structure; hyaluronic acid
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Mgr. Pavla Foltynová, Ph.D., učo 106624. Změněno: 15. 3. 2021 21:09.
Anotace
The mechanical and biomechanical properties of the cell's extracellular matrix is a factor in their later development. In this regard, elastic biocompatible materials as a surface for cultivating cells are in recent years, becoming an exciting field of study. Hydrogels are a hydrophilic 3D network of synthetic polymers or biopolymers. They can have tunable mechanical properties, controllable degradability, or they can be designed for long-time release of treatments. Here, hydrogels from Hyaluronic acid (HA), Bovine serum albumin (BSA) and gelatin was prepared, via carbodiimide chemistry. Morphology and mechanical properties of these hydrogels was characterized via Atomic Force Microscopy (AFM). Young's modulus of HA/BSA and HA/BSA/gel were 124.7 ± 15.9 and 20.3 ± 0.5 kPa, respectively. Mouse embryonic fibroblasts were then cultivated on hydrogels and on the glass surface as a reference. Mechanical and morphological properties of fibroblasts were examined by AFM and via phalloidin-TRITC staying. Relationship between the structure of cells and the type of surface was seen. Cells grown on glass were more flattened with an evenly linear actin structure. On hydrogels, fibroblasts were more star-shaped with non-linear actin structures and many filopodia. Furthermore, height of cells was higher was lower compared to cells on both types of hydrogels (0.36 µm vs. 0.56 µm vs. 1.03 µm). Young's modulus of cells on fibroblast was highest, which relates to the whole range of structural changes linked with surface stiffness. In conclusion, this type of hydrogel might be utilized in the future as a better surface material for cell cultivation.
Návaznosti
LM2018127, projekt VaVNázev: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)
Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology
VytisknoutZobrazeno: 13. 5. 2024 02:54