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@inproceedings{1752518,
author = {Přibyl, Jan and Rotrekl, Vladimír and Pešl, Martin and Jelínková, Šárka and Kratochvílová, Irena},
address = {Brno},
booktitle = {Nanocon 2020},
doi = {http://dx.doi.org/10.37904/nanocon.2020.3740},
edition = {2020},
keywords = {Atomic Force Microscopy; Mechanical Mapping; Cell stiffness; Cryopreservation},
howpublished = {elektronická verze "online"},
language = {eng},
location = {Brno},
pages = {1-6},
publisher = {TANGER Ltd.},
title = {TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES},
url = {https://www.confer.cz/nanocon/2020/3740-time-lapse-monitoring-of-cell-mechanical-properties},
year = {2020}
}
TY - JOUR
ID - 1752518
AU - Přibyl, Jan - Rotrekl, Vladimír - Pešl, Martin - Jelínková, Šárka - Kratochvílová, Irena
PY - 2020
TI - TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES
PB - TANGER Ltd.
CY - Brno
KW - Atomic Force Microscopy
KW - Mechanical Mapping
KW - Cell stiffness
KW - Cryopreservation
UR - https://www.confer.cz/nanocon/2020/3740-time-lapse-monitoring-of-cell-mechanical-properties
N2 - Atomic force microscopy (AFM) is a highly sensitive non-invasive surface method able to provide insight into cells' mechanical parameters. Membrane and sub-membrane development, as well as internal cellular properties, can be monitored. The stiffness of cells is a fundamental phenomenon that reflects changes in cell physiology. More importantly, changes in cell mechanical properties are also often found to be closely associated with various disease conditions. Cell mechanics are mainly dependent on cytoskeletal architecture. The development of cryopreserved cells' mechanical properties (stiffness) after thawing was studied using AFM. Cell stiffness was mapped and thus monitored in time and space under nearly physiological conditions (i.e., in culture medium and at elevated temperature). In AFM force spectroscopy mode, cells are indented at many sites, and their complete elastic responses are recorded, enabling them to reconstruct a stiffness map. We measured the frozen cell surface stiffness immediately after thawing; they, when the dynamics of development of the cell stiffness were monitored in time up to 24 hours. Moreover, the AFM spectroscopy was combined with fluorescence-based staining of the cytoskeleton, thus enabling to directly correlate cytoskeleton development with stiffness mapping
ER -
PŘIBYL, Jan, Vladimír ROTREKL, Martin PEŠL, Šárka JELÍNKOVÁ a Irena KRATOCHVÍLOVÁ. TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES. Online. In \textit{Nanocon 2020}. 2020. vyd. Brno: TANGER Ltd., 2020, s.~1-6. ISSN~2694-930X. Dostupné z: https://dx.doi.org/10.37904/nanocon.2020.3740.
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