TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES

D 2020

TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES

PŘIBYL, Jan, Vladimír ROTREKL, Martin PEŠL, Šárka JELÍNKOVÁ, Irena KRATOCHVÍLOVÁ et. al.

Základní údaje

Originální název

TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES

Autoři

PŘIBYL, Jan (203 Česká republika, domácí), Vladimír ROTREKL (203 Česká republika, domácí), Martin PEŠL (203 Česká republika, domácí), Šárka JELÍNKOVÁ (203 Česká republika, domácí) a Irena KRATOCHVÍLOVÁ (garant)

Vydání

2020. vyd. Brno, Nanocon 2020, od s. 1-6, 6 s. 2020

Nakladatel

TANGER Ltd.

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10601 Cell biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Odkazy

URL

Kód RIV

RIV/00216224:14740/20:00134615

Organizační jednotka

Středoevropský technologický institut

ISSN

DOI

http://dx.doi.org/10.37904/nanocon.2020.3740

UT WoS

000664505500071

Klíčová slova anglicky

Atomic Force Microscopy; Mechanical Mapping; Cell stiffness; Cryopreservation

Štítky

CF NANO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 10. 2024 09:40, Ing. Monika Szurmanová, Ph.D.

Anotace

V originále

Atomic force microscopy (AFM) is a highly sensitive non-invasive surface method able to provide insight into cells' mechanical parameters. Membrane and sub-membrane development, as well as internal cellular properties, can be monitored. The stiffness of cells is a fundamental phenomenon that reflects changes in cell physiology. More importantly, changes in cell mechanical properties are also often found to be closely associated with various disease conditions. Cell mechanics are mainly dependent on cytoskeletal architecture. The development of cryopreserved cells' mechanical properties (stiffness) after thawing was studied using AFM. Cell stiffness was mapped and thus monitored in time and space under nearly physiological conditions (i.e., in culture medium and at elevated temperature). In AFM force spectroscopy mode, cells are indented at many sites, and their complete elastic responses are recorded, enabling them to reconstruct a stiffness map. We measured the frozen cell surface stiffness immediately after

Návaznosti

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

NU20-06-00156, projekt VaV

Název: Vliv pneumologické medikace na funkce lidských kardiomyocytů.

Investor: Ministerstvo zdravotnictví ČR, Vliv pneumologické medikace na funkce lidských kardiomyocytů, Podprogram 1 - standardní

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

PŘIBYL, Jan, Vladimír ROTREKL, Martin PEŠL, Šárka JELÍNKOVÁ a Irena KRATOCHVÍLOVÁ. TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES. Online. In Nanocon 2020. 2020. vyd. Brno: TANGER Ltd., 2020, s. 1-6. ISSN 2694-930X. Dostupné z: https://dx.doi.org/10.37904/nanocon.2020.3740.

@inproceedings{1752518,
   author = {Přibyl, Jan and Rotrekl, Vladimír and Pešl, Martin and Jelínková, Šárka and Kratochvílová, Irena},
   address = {Brno},
   booktitle = {Nanocon 2020},
   doi = {http://dx.doi.org/10.37904/nanocon.2020.3740},
   edition = {2020},
   keywords = {Atomic Force Microscopy; Mechanical Mapping; Cell stiffness; Cryopreservation},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Brno},
   pages = {1-6},
   publisher = {TANGER Ltd.},
   title = {TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES},
   url = {https://www.confer.cz/nanocon/2020/3740-time-lapse-monitoring-of-cell-mechanical-properties},
   year = {2020}
}

TY  - JOUR
ID  - 1752518
AU  - Přibyl, Jan - Rotrekl, Vladimír - Pešl, Martin - Jelínková, Šárka - Kratochvílová, Irena
PY  - 2020
TI  - TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES
PB  - TANGER Ltd.
CY  - Brno
KW  - Atomic Force Microscopy
KW  - Mechanical Mapping
KW  - Cell stiffness
KW  - Cryopreservation
UR  - https://www.confer.cz/nanocon/2020/3740-time-lapse-monitoring-of-cell-mechanical-properties
N2  - Atomic force microscopy (AFM) is a highly sensitive non-invasive surface method able to provide insight into cells' mechanical parameters. Membrane and sub-membrane development, as well as internal cellular properties, can be monitored. The stiffness of cells is a fundamental phenomenon that reflects changes in cell physiology. More importantly, changes in cell mechanical properties are also often found to be closely associated with various disease conditions. Cell mechanics are mainly dependent on cytoskeletal architecture. The development of cryopreserved cells' mechanical properties (stiffness) after thawing was studied using AFM. Cell stiffness was mapped and thus monitored in time and space under nearly physiological conditions (i.e., in culture medium and at elevated temperature). In AFM force spectroscopy mode, cells are indented at many sites, and their complete elastic responses are recorded, enabling them to reconstruct a stiffness map. We measured the frozen cell surface stiffness immediately after
ER  -

PŘIBYL, Jan, Vladimír ROTREKL, Martin PEŠL, Šárka JELÍNKOVÁ a Irena KRATOCHVÍLOVÁ. TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES. Online. In \textit{Nanocon 2020}. 2020. vyd. Brno: TANGER Ltd., 2020, s.~1-6. ISSN~2694-930X. Dostupné z: https://dx.doi.org/10.37904/nanocon.2020.3740.

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