TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES

D 2020

TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES

PŘIBYL, Jan, Vladimír ROTREKL, Martin PEŠL, Šárka JELÍNKOVÁ, Irena KRATOCHVÍLOVÁ et. al.

Basic information

Original name

TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES

Authors

PŘIBYL, Jan (203 Czech Republic, belonging to the institution), Vladimír ROTREKL (203 Czech Republic, belonging to the institution), Martin PEŠL (203 Czech Republic, belonging to the institution), Šárka JELÍNKOVÁ (203 Czech Republic, belonging to the institution) and Irena KRATOCHVÍLOVÁ (guarantor)

Edition

2020. vyd. Brno, Nanocon 2020, p. 1-6, 6 pp. 2020

Publisher

TANGER Ltd.

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10601 Cell biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

electronic version available online

References:

URL

RIV identification code

RIV/00216224:14740/20:00134615

Organization unit

Central European Institute of Technology

ISSN

DOI

http://dx.doi.org/10.37904/nanocon.2020.3740

UT WoS

000664505500071

Keywords in English

Atomic Force Microscopy; Mechanical Mapping; Cell stiffness; Cryopreservation

Abstract

V originále

Atomic force microscopy (AFM) is a highly sensitive non-invasive surface method able to provide insight into cells' mechanical parameters. Membrane and sub-membrane development, as well as internal cellular properties, can be monitored. The stiffness of cells is a fundamental phenomenon that reflects changes in cell physiology. More importantly, changes in cell mechanical properties are also often found to be closely associated with various disease conditions. Cell mechanics are mainly dependent on cytoskeletal architecture. The development of cryopreserved cells' mechanical properties (stiffness) after thawing was studied using AFM. Cell stiffness was mapped and thus monitored in time and space under nearly physiological conditions (i.e., in culture medium and at elevated temperature). In AFM force spectroscopy mode, cells are indented at many sites, and their complete elastic responses are recorded, enabling them to reconstruct a stiffness map. We measured the frozen cell surface stiffness immediately after

Links

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

NU20-06-00156, research and development project

Name: Vliv pneumologické medikace na funkce lidských kardiomyocytů.

Investor: Ministry of Health of the CR, Subprogram 1 - standard

90127, large research infrastructures

Name: CIISB II

Citovat

PŘIBYL, Jan, Vladimír ROTREKL, Martin PEŠL, Šárka JELÍNKOVÁ and Irena KRATOCHVÍLOVÁ. TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES. Online. In Nanocon 2020. 2020th ed. Brno: TANGER Ltd., 2020, p. 1-6. ISSN 2694-930X. Available from: https://dx.doi.org/10.37904/nanocon.2020.3740.

@inproceedings{1752518,
   author = {Přibyl, Jan and Rotrekl, Vladimír and Pešl, Martin and Jelínková, Šárka and Kratochvílová, Irena},
   address = {Brno},
   booktitle = {Nanocon 2020},
   doi = {http://dx.doi.org/10.37904/nanocon.2020.3740},
   edition = {2020},
   keywords = {Atomic Force Microscopy; Mechanical Mapping; Cell stiffness; Cryopreservation},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Brno},
   pages = {1-6},
   publisher = {TANGER Ltd.},
   title = {TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES},
   url = {https://www.confer.cz/nanocon/2020/3740-time-lapse-monitoring-of-cell-mechanical-properties},
   year = {2020}
}

TY  - JOUR
ID  - 1752518
AU  - Přibyl, Jan - Rotrekl, Vladimír - Pešl, Martin - Jelínková, Šárka - Kratochvílová, Irena
PY  - 2020
TI  - TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES
PB  - TANGER Ltd.
CY  - Brno
KW  - Atomic Force Microscopy
KW  - Mechanical Mapping
KW  - Cell stiffness
KW  - Cryopreservation
UR  - https://www.confer.cz/nanocon/2020/3740-time-lapse-monitoring-of-cell-mechanical-properties
N2  - Atomic force microscopy (AFM) is a highly sensitive non-invasive surface method able to provide insight into cells' mechanical parameters. Membrane and sub-membrane development, as well as internal cellular properties, can be monitored. The stiffness of cells is a fundamental phenomenon that reflects changes in cell physiology. More importantly, changes in cell mechanical properties are also often found to be closely associated with various disease conditions. Cell mechanics are mainly dependent on cytoskeletal architecture. The development of cryopreserved cells' mechanical properties (stiffness) after thawing was studied using AFM. Cell stiffness was mapped and thus monitored in time and space under nearly physiological conditions (i.e., in culture medium and at elevated temperature). In AFM force spectroscopy mode, cells are indented at many sites, and their complete elastic responses are recorded, enabling them to reconstruct a stiffness map. We measured the frozen cell surface stiffness immediately after
ER  -

PŘIBYL, Jan, Vladimír ROTREKL, Martin PEŠL, Šárka JELÍNKOVÁ and Irena KRATOCHVÍLOVÁ. TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES. Online. In \textit{Nanocon 2020}. 2020th ed. Brno: TANGER Ltd., 2020, p.~1-6. ISSN~2694-930X. Available from: https://dx.doi.org/10.37904/nanocon.2020.3740.

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