a 2020

Human Telomere Repeat Binding Factor TRF1 Replaces TRF2 Bound to Shelterin Core Hub TIN2 when TPP1 Is Absent

JANOVIČ, Tomáš and Ctirad HOFR

Basic information

Original name

Human Telomere Repeat Binding Factor TRF1 Replaces TRF2 Bound to Shelterin Core Hub TIN2 when TPP1 Is Absent

Authors

JANOVIČ, Tomáš (203 Czech Republic, belonging to the institution) and Ctirad HOFR (203 Czech Republic, belonging to the institution)

Edition

CEITEC PhD Conference, 2020

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

RIV identification code

RIV/00216224:14740/20:00118402

Organization unit

Central European Institute of Technology

Keywords in English

TRF1; TRF2

Tags

Změněno: 11/10/2024 12:12, Ing. Marie Švancarová

Abstract

V originále

Telomeric repeat binding factor TRF1, TRF2 together with TIN2 protein create important roles as a shelterin-core subunits. Their dynamics is important for regulating the assembly of shelterin complex. We applied FCCS – Fluorescence Cross-Correlation Spectroscopy as an in vitro single molecule fluorescence microscopy approach to quantitatively describe the exchange of TRF1 and TRF2 in complex of TIN2. We found that TRF1 can effectively exchange TRF2 in TIN2-TRF2 complex which is essential regarding the function of shelterin during specific recognition of chromosome ends and telomerase activity regulation. We extended the FCCS study with addition of TPP1 which is TIN2 binding partner and tested whether TPP1 presence could change the TIN2-TRF2 interaction and enable TIN2 interact simultaneously with TRF1 and TRF2, hence allow to originate TRF1-TIN2- TRF2 shelterin-core complex. Our FCCS data shows that TPP1 indeed upon binding to TIN2 induce allosteric effect improving the binding capacity so the complex TPP1-TIN2 can accommodate both TRF1 and TRF2 and it is essential for the proper formation of TRF1-TIN2-TRF2 shelterin-core complex.

Links

LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
90043, large research infrastructures
Name: CIISB