Tuning of SPR for Colocalized Characterization of Biomolecules
Using Nanoparticle-Containing Multilayers

J 2021

Tuning of SPR for Colocalized Characterization of Biomolecules Using Nanoparticle-Containing Multilayers

DVOŘÁK, Jan, Ondřej CAHA and Dušan HEMZAL

Basic information

Original name

Tuning of SPR for Colocalized Characterization of Biomolecules Using Nanoparticle-Containing Multilayers

Authors

DVOŘÁK, Jan (203 Czech Republic, belonging to the institution), Ondřej CAHA (203 Czech Republic, belonging to the institution) and Dušan HEMZAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plasmonics, New York, Springer, 2021, 1557-1955

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10302 Condensed matter physics

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.726

RIV identification code

RIV/00216224:14310/21:00121254

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s11468-021-01383-z

UT WoS

000614701700001

Keywords in English

Surface plasmon resonance (SPR); Total internal reflection ellipsometry (TIRE); Nanoparticles; Rtt103 160; protein

Abstract

V originále

In this contribution, we quantify the fine-tuning of surface plasmon resonance via nanoparticle coating of gold SPR chips. We target preparation of atomically flat surface with defined charge, needed for scanning probe characterization of biomolecules, with simultaneous optimization of in situ SPR sensitivity during immobilization of these molecules. Using total internal reflection ellipsometry, we show that the goal can be achieved by combination of charge-stabilized silver nanoparticles over underlying gold chip of reduced thickness. The provided formulas for optimal silver to gold thickness relation are valid for arbitrary analyte and the method was experimentally verified on binding of Rtt103 protein.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

Citovat

DVOŘÁK, Jan, Ondřej CAHA and Dušan HEMZAL. Tuning of SPR for Colocalized Characterization of Biomolecules Using Nanoparticle-Containing Multilayers. Plasmonics. New York: Springer, 2021, vol. 16, No 4, p. 1203-1211. ISSN 1557-1955. Available from: https://dx.doi.org/10.1007/s11468-021-01383-z.

@article{1753176,
   author = {Dvořák, Jan and Caha, Ondřej and Hemzal, Dušan},
   article_location = {New York},
   article_number = {4},
   doi = {http://dx.doi.org/10.1007/s11468-021-01383-z},
   keywords = {Surface plasmon resonance (SPR); Total internal reflection ellipsometry (TIRE); Nanoparticles; Rtt103 160; protein},
   language = {eng},
   issn = {1557-1955},
   journal = {Plasmonics},
   title = {Tuning of SPR for Colocalized Characterization of Biomolecules Using Nanoparticle-Containing Multilayers},
   url = {https://doi.org/10.1007/s11468-021-01383-z},
   volume = {16},
   year = {2021}
}

TY  - JOUR
ID  - 1753176
AU  - Dvořák, Jan - Caha, Ondřej - Hemzal, Dušan
PY  - 2021
TI  - Tuning of SPR for Colocalized Characterization of Biomolecules Using Nanoparticle-Containing Multilayers
JF  - Plasmonics
VL  - 16
IS  - 4
SP  - 1203-1211
EP  - 1203-1211
PB  - Springer
SN  - 15571955
KW  - Surface plasmon resonance (SPR)
KW  - Total internal reflection ellipsometry (TIRE)
KW  - Nanoparticles
KW  - Rtt103 160
KW  - protein
UR  - https://doi.org/10.1007/s11468-021-01383-z
N2  - In this contribution, we quantify the fine-tuning of surface plasmon resonance via nanoparticle coating of gold SPR chips. We target preparation of atomically flat surface with defined charge, needed for scanning probe characterization of biomolecules, with simultaneous optimization of in situ SPR sensitivity during immobilization of these molecules. Using total internal reflection ellipsometry, we show that the goal can be achieved by combination of charge-stabilized silver nanoparticles over underlying gold chip of reduced thickness. The provided formulas for optimal silver to gold thickness relation are valid for arbitrary analyte and the method was experimentally verified on binding of Rtt103 protein.
ER  -

DVOŘÁK, Jan, Ondřej CAHA and Dušan HEMZAL. Tuning of SPR for Colocalized Characterization of Biomolecules Using Nanoparticle-Containing Multilayers. \textit{Plasmonics}. New York: Springer, 2021, vol.~16, No~4, p.~1203-1211. ISSN~1557-1955. Available from: https://dx.doi.org/10.1007/s11468-021-01383-z.

Detailed Information on Publication Record