Upconversion nanoparticles as labels for histopathological
tissue evaluation

a 2021

Upconversion nanoparticles as labels for histopathological tissue evaluation

MICKERT, Matthias Jürgen, Zdeněk FARKA, Krzysztof KRAWCZYK, Antonín HLAVÁČEK, Julian BRANDMEIER et. al.

Základní údaje

Originální název

Upconversion nanoparticles as labels for histopathological tissue evaluation

Autoři

MICKERT, Matthias Jürgen, Zdeněk FARKA, Krzysztof KRAWCZYK, Antonín HLAVÁČEK, Julian BRANDMEIER, Stefan ANDERSSON-ENGELS, Anders SJÖGREN, Petr SKLÁDAL a Hans-Heiner GORRIS

Vydání

UPCONline 2021, 2021

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10406 Analytical chemistry

Stát vydavatele

Francie

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Přírodovědecká fakulta

Příznaky

Mezinárodní význam

Změněno: 10. 5. 2021 22:49, doc. Mgr. Zdeněk Farka, Ph.D.

Anotace

V originále

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are routine methods for the microscopic identification of cancerous cells in cell cultures and histological tissues. A growing population and longer life expectancy lead to an increase in tissue samples from cancer patients. Multiplexing and digital pathology can increase the sample throughput, improve the diagnosis, and reduce the analysis time. UCNPs are well suited for digital image analysis of biological tissues because the anti-Stokes emission under NIR excitation drastically reduces autofluorescence and light scattering, and their multiple narrow emission bands allow for multiplexed applications. In this work, we designed streptavidin-modified UCNPs (SA-UCNP) for the detection of the breast cancer biomarker HER2 on the surface of model breast cancer cell lines and breast cancer tissue. The SA-UCNP conjugate achieved a significantly higher signal-to-background ratio compared to a conventional labelling using a streptavidin-carboxyfluorescein conjugate under the same experimental conditions. An in-house made UCNP-conjugate from Lumito was used to label HER2 in breast cancer tissue. The imaging was performed by Lumito’s prototype scanner capable of combining UCNP upconversion imaging and haematoxylin brightfield imaging at a 20× magnification.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

LTAB19011, projekt VaV

Název: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel, INTER-ACTION

Citovat

MICKERT, Matthias Jürgen, Zdeněk FARKA, Krzysztof KRAWCZYK, Antonín HLAVÁČEK, Julian BRANDMEIER, Stefan ANDERSSON-ENGELS, Anders SJÖGREN, Petr SKLÁDAL a Hans-Heiner GORRIS. Upconversion nanoparticles as labels for histopathological tissue evaluation. In UPCONline 2021. 2021.

@proceedings{1757237,
   author = {Mickert, Matthias Jürgen and Farka, Zdeněk and Krawczyk, Krzysztof and Hlaváček, Antonín and Brandmeier, Julian and AnderssonandEngels, Stefan and Sjögren, Anders and Skládal, Petr and Gorris, HansandHeiner},
   booktitle = {UPCONline 2021},
   language = {eng},
   title = {Upconversion nanoparticles as labels for histopathological tissue evaluation},
   year = {2021}
}

TY  - CONF
ID  - 1757237
AU  - Mickert, Matthias Jürgen - Farka, Zdeněk - Krawczyk, Krzysztof - Hlaváček, Antonín - Brandmeier, Julian - Andersson-Engels, Stefan - Sjögren, Anders - Skládal, Petr - Gorris, Hans-Heiner
PY  - 2021
TI  - Upconversion nanoparticles as labels for histopathological tissue evaluation
N2  - Immunocytochemistry (ICC) and immunohistochemistry (IHC) are routine methods for the microscopic identification of cancerous cells in cell cultures and histological tissues. A growing population and longer life expectancy lead to an increase in tissue samples from cancer patients. Multiplexing and digital pathology can increase the sample throughput, improve the diagnosis, and reduce the analysis time. UCNPs are well suited for digital image analysis of biological tissues because the anti-Stokes emission under NIR excitation drastically reduces autofluorescence and light scattering, and their multiple narrow emission bands allow for multiplexed applications. In this work, we designed streptavidin-modified UCNPs (SA-UCNP) for the detection of the breast cancer biomarker HER2 on the surface of model breast cancer cell lines and breast cancer tissue. The SA-UCNP conjugate achieved a significantly higher signal-to-background ratio compared to a conventional labelling using a streptavidin-carboxyfluorescein conjugate under the same experimental conditions. An in-house made UCNP-conjugate from Lumito was used to label HER2 in breast cancer tissue. The imaging was performed by Lumito’s prototype scanner capable of combining UCNP upconversion imaging and haematoxylin brightfield imaging at a 20× magnification.
ER  -

Podrobný výpis o publikaci