Další formáty:
BibTeX
LaTeX
RIS
@article{1758102,
author = {Capoor, M. N. and Konieczna, Anna and McDowell, A. and Růžička, Filip and Smrčka, Martin and Jančálek, Radim and Máca, Karel and Lujc, Michael and Ahmed, Fatima and Birkenmaier, C. and Dudli, S. and Slabý, Ondřej},
article_location = {Basel},
article_number = {5},
doi = {http://dx.doi.org/10.3390/ijms22052347},
keywords = {Cutibacterium acnes; disc cells; co-culture; inflammation; neurotrophic factors; gene expression; intracellular},
language = {eng},
issn = {1422-0067},
journal = {International Journal of Molecular Sciences},
title = {Pro-Inflammatory and Neurotrophic Factor Responses of Cells Derived from Degenerative Human Intervertebral Discs to the Opportunistic Pathogen Cutibacterium acnes},
url = {https://www.mdpi.com/1422-0067/22/5/2347},
volume = {22},
year = {2021}
}
TY - JOUR
ID - 1758102
AU - Capoor, M. N. - Konieczna, Anna - McDowell, A. - Růžička, Filip - Smrčka, Martin - Jančálek, Radim - Máca, Karel - Lujc, Michael - Ahmed, Fatima - Birkenmaier, C. - Dudli, S. - Slabý, Ondřej
PY - 2021
TI - Pro-Inflammatory and Neurotrophic Factor Responses of Cells Derived from Degenerative Human Intervertebral Discs to the Opportunistic Pathogen Cutibacterium acnes
JF - International Journal of Molecular Sciences
VL - 22
IS - 5
SP - 1-16
EP - 1-16
PB - MDPI
SN - 14220067
KW - Cutibacterium acnes
KW - disc cells
KW - co-culture
KW - inflammation
KW - neurotrophic factors
KW - gene expression
KW - intracellular
UR - https://www.mdpi.com/1422-0067/22/5/2347
N2 - Previously, we proposed the hypothesis that similarities in the inflammatory response observed in acne vulgaris and degenerative disc disease (DDD), especially the central role of interleukin (IL)-1 beta, may be further evidence of the role of the anaerobic bacterium Cutibacterium (previously Propionibacterium) acnes in the underlying aetiology of disc degeneration. To investigate this, we examined the upregulation of IL-1 beta, and other known IL-1 beta-induced inflammatory markers and neurotrophic factors, from nucleus-pulposus-derived disc cells infected in vitro with C. acnes for up to 48 h. Upon infection, significant upregulation of IL-1 beta, alongside IL-6, IL-8, chemokine (C-C motif) ligand 3 (CCL3), chemokine (C-C motif) ligand 4 (CCL4), nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF), was observed with cells isolated from the degenerative discs of eight patients versus non-infected controls. Expression levels did, however, depend on gene target, multiplicity and period of infection and, notably, donor response. Pre-treatment of cells with clindamycin prior to infection significantly reduced the production of pro-inflammatory mediators. This study confirms that C. acnes can stimulate the expression of IL-1 beta and other host molecules previously associated with pathological changes in disc tissue, including neo-innervation. While still controversial, the role of C. acnes in DDD remains biologically credible, and its ability to cause disease likely reflects a combination of factors, particularly individualised response to infection.
ER -
CAPOOR, M. N., Anna KONIECZNA, A. MCDOWELL, Filip RŮŽIČKA, Martin SMRČKA, Radim JANČÁLEK, Karel MÁCA, Michael LUJC, Fatima AHMED, C. BIRKENMAIER, S. DUDLI a Ondřej SLABÝ. Pro-Inflammatory and Neurotrophic Factor Responses of Cells Derived from Degenerative Human Intervertebral Discs to the Opportunistic Pathogen Cutibacterium acnes. \textit{International Journal of Molecular Sciences}. Basel: MDPI, 2021, roč.~22, č.~5, s.~1-16. ISSN~1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms22052347.
|
