Pro-Inflammatory and Neurotrophic Factor Responses of Cells
Derived from Degenerative Human Intervertebral Discs to the
Opportunistic Pathogen Cutibacterium acnes

J 2021

Pro-Inflammatory and Neurotrophic Factor Responses of Cells Derived from Degenerative Human Intervertebral Discs to the Opportunistic Pathogen Cutibacterium acnes

CAPOOR, M. N., Anna KONIECZNA, A. MCDOWELL, Filip RŮŽIČKA, Martin SMRČKA et. al.

Basic information

Original name

Pro-Inflammatory and Neurotrophic Factor Responses of Cells Derived from Degenerative Human Intervertebral Discs to the Opportunistic Pathogen Cutibacterium acnes

Authors

CAPOOR, M. N., Anna KONIECZNA (203 Czech Republic, belonging to the institution), A. MCDOWELL, Filip RŮŽIČKA (203 Czech Republic, belonging to the institution), Martin SMRČKA (203 Czech Republic, belonging to the institution), Radim JANČÁLEK (203 Czech Republic, belonging to the institution), Karel MÁCA (203 Czech Republic, belonging to the institution), Michael LUJC (203 Czech Republic, belonging to the institution), Fatima AHMED (586 Pakistan), C. BIRKENMAIER, S. DUDLI and Ondřej SLABÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

International Journal of Molecular Sciences, Basel, MDPI, 2021, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.208

RIV identification code

RIV/00216224:14110/21:00121370

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/ijms22052347

UT WoS

000628279100001

Keywords in English

Cutibacterium acnes; disc cells; co-culture; inflammation; neurotrophic factors; gene expression; intracellular

Abstract

V originále

Previously, we proposed the hypothesis that similarities in the inflammatory response observed in acne vulgaris and degenerative disc disease (DDD), especially the central role of interleukin (IL)-1 beta, may be further evidence of the role of the anaerobic bacterium Cutibacterium (previously Propionibacterium) acnes in the underlying aetiology of disc degeneration. To investigate this, we examined the upregulation of IL-1 beta, and other known IL-1 beta-induced inflammatory markers and neurotrophic factors, from nucleus-pulposus-derived disc cells infected in vitro with C. acnes for up to 48 h. Upon infection, significant upregulation of IL-1 beta, alongside IL-6, IL-8, chemokine (C-C motif) ligand 3 (CCL3), chemokine (C-C motif) ligand 4 (CCL4), nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF), was observed with cells isolated from the degenerative discs of eight patients versus non-infected controls. Expression levels did, however, depend on gene target, multiplicity and period of infection and, notably, donor response. Pre-treatment of cells with clindamycin prior to infection significantly reduced the production of pro-inflammatory mediators. This study confirms that C. acnes can stimulate the expression of IL-1 beta and other host molecules previously associated with pathological changes in disc tissue, including neo-innervation. While still controversial, the role of C. acnes in DDD remains biologically credible, and its ability to cause disease likely reflects a combination of factors, particularly individualised response to infection.

Citovat

CAPOOR, M. N., Anna KONIECZNA, A. MCDOWELL, Filip RŮŽIČKA, Martin SMRČKA, Radim JANČÁLEK, Karel MÁCA, Michael LUJC, Fatima AHMED, C. BIRKENMAIER, S. DUDLI and Ondřej SLABÝ. Pro-Inflammatory and Neurotrophic Factor Responses of Cells Derived from Degenerative Human Intervertebral Discs to the Opportunistic Pathogen Cutibacterium acnes. International Journal of Molecular Sciences. Basel: MDPI, 2021, vol. 22, No 5, p. 1-16. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms22052347.

@article{1758102,
   author = {Capoor, M. N. and Konieczna, Anna and McDowell, A. and Růžička, Filip and Smrčka, Martin and Jančálek, Radim and Máca, Karel and Lujc, Michael and Ahmed, Fatima and Birkenmaier, C. and Dudli, S. and Slabý, Ondřej},
   article_location = {Basel},
   article_number = {5},
   doi = {http://dx.doi.org/10.3390/ijms22052347},
   keywords = {Cutibacterium acnes; disc cells; co-culture; inflammation; neurotrophic factors; gene expression; intracellular},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Pro-Inflammatory and Neurotrophic Factor Responses of Cells Derived from Degenerative Human Intervertebral Discs to the Opportunistic Pathogen Cutibacterium acnes},
   url = {https://www.mdpi.com/1422-0067/22/5/2347},
   volume = {22},
   year = {2021}
}

TY  - JOUR
ID  - 1758102
AU  - Capoor, M. N. - Konieczna, Anna - McDowell, A. - Růžička, Filip - Smrčka, Martin - Jančálek, Radim - Máca, Karel - Lujc, Michael - Ahmed, Fatima - Birkenmaier, C. - Dudli, S. - Slabý, Ondřej
PY  - 2021
TI  - Pro-Inflammatory and Neurotrophic Factor Responses of Cells Derived from Degenerative Human Intervertebral Discs to the Opportunistic Pathogen Cutibacterium acnes
JF  - International Journal of Molecular Sciences
VL  - 22
IS  - 5
SP  - 1-16
EP  - 1-16
PB  - MDPI
SN  - 14220067
KW  - Cutibacterium acnes
KW  - disc cells
KW  - co-culture
KW  - inflammation
KW  - neurotrophic factors
KW  - gene expression
KW  - intracellular
UR  - https://www.mdpi.com/1422-0067/22/5/2347
N2  - Previously, we proposed the hypothesis that similarities in the inflammatory response observed in acne vulgaris and degenerative disc disease (DDD), especially the central role of interleukin (IL)-1 beta, may be further evidence of the role of the anaerobic bacterium Cutibacterium (previously Propionibacterium) acnes in the underlying aetiology of disc degeneration. To investigate this, we examined the upregulation of IL-1 beta, and other known IL-1 beta-induced inflammatory markers and neurotrophic factors, from nucleus-pulposus-derived disc cells infected in vitro with C. acnes for up to 48 h. Upon infection, significant upregulation of IL-1 beta, alongside IL-6, IL-8, chemokine (C-C motif) ligand 3 (CCL3), chemokine (C-C motif) ligand 4 (CCL4), nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF), was observed with cells isolated from the degenerative discs of eight patients versus non-infected controls. Expression levels did, however, depend on gene target, multiplicity and period of infection and, notably, donor response. Pre-treatment of cells with clindamycin prior to infection significantly reduced the production of pro-inflammatory mediators. This study confirms that C. acnes can stimulate the expression of IL-1 beta and other host molecules previously associated with pathological changes in disc tissue, including neo-innervation. While still controversial, the role of C. acnes in DDD remains biologically credible, and its ability to cause disease likely reflects a combination of factors, particularly individualised response to infection.
ER  -

CAPOOR, M. N., Anna KONIECZNA, A. MCDOWELL, Filip RŮŽIČKA, Martin SMRČKA, Radim JANČÁLEK, Karel MÁCA, Michael LUJC, Fatima AHMED, C. BIRKENMAIER, S. DUDLI and Ondřej SLABÝ. Pro-Inflammatory and Neurotrophic Factor Responses of Cells Derived from Degenerative Human Intervertebral Discs to the Opportunistic Pathogen Cutibacterium acnes. \textit{International Journal of Molecular Sciences}. Basel: MDPI, 2021, vol.~22, No~5, p.~1-16. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms22052347.

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