Actinotignum schaalii: Relation to Concomitants and Connection
to Patients’ Conditions in Polymicrobial Biofilms of Urinary
Tract Catheters and Urines

J 2021

Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines

KOTÁSKOVÁ, Iva, Vít SYROVÁTKA, Hana OBRUČOVÁ, Petra VÍDEŇSKÁ, Barbora ZWINSOVÁ et. al.

Základní údaje

Originální název

Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines

Autoři

KOTÁSKOVÁ, Iva (203 Česká republika, domácí), Vít SYROVÁTKA (203 Česká republika, domácí), Hana OBRUČOVÁ (203 Česká republika), Petra VÍDEŇSKÁ (203 Česká republika, domácí), Barbora ZWINSOVÁ (203 Česká republika, domácí), Veronika HOLÁ (203 Česká republika, domácí), Eva BLAŠTÍKOVÁ (203 Česká republika), Filip RŮŽIČKA (203 Česká republika, domácí) a Tomáš FREIBERGER (203 Česká republika, garant, domácí)

Vydání

Microorganisms, Basel, MDPI, 2021, 2076-2607

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.926

Kód RIV

RIV/00216224:14110/21:00120090

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3390/microorganisms9030669

UT WoS

000635249300001

Klíčová slova anglicky

Actinotignum; Actinobaculum; hydronephrosis; urinary catheter; ureteral stent; Double-J catheter; Propionimicrobium; Fusobacterium; urobiome; microbiome; colonisation

Štítky

14110113, 14110114, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 2. 2023 21:10, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Actinotignum schaalii is an emerging, opportunistic pathogen and its connection to non-infectious diseases and conditions, such as prostate or bladder cancer, or chronic inflammation has been proposed. Here, we analyzed 297 urine, ureteral and urinary catheter samples from 128 patients by Polymerase Chain Reaction followed by Denaturing Gradient Gel Electrophoresis and Sequencing (PCR-DGGE-S), and culture, and 29 of these samples also by 16S rRNA Illumina sequencing, to establish A. schaalii’s prevalence in urinary tract-related samples, its relation to other bacteria, and its potential association with patients’ conditions and samples’ characteristics. A. schaalii-positive samples were significantly more diverse than A. schaalii negative and between-group diversity was higher than intra-group. Propionimicrobium lymphophilum, Fusobacterium nucleatum, Veillonella sp., Morganella sp., and Aerococcus sp. were significantly more often present in A. schaalii-positive samples; thus, we suggest these species are A. schaalii’s concomitants, while Enterobacter and Staphylococcaceae were more often identified in A. schaalii-negative samples; therefore, we propose A. schaalii and these species are mutually exclusive. Additionally, a significantly higher A. schaalii prevalence in patients with ureter stricture associated hydronephrosis (p = 0.020) was noted. We suggest that A. schaalii could be an early polybacterial biofilm colonizer, together with concomitant species, known for pro-inflammatory features.

Návaznosti

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

LM2015085, projekt VaV

Název: CERIT Scientific Cloud (Akronym: CERIT-SC)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CERIT Scientific Cloud

MUNI/A/1099/2019, interní kód MU

Název: Faktory nespecifické imunity u některých imunopatologických stavů (Akronym: nespecif. imunita)

Investor: Masarykova univerzita, Faktory nespecifické imunity u některých imunopatologických stavů, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

MUNI/A/1412/2020, interní kód MU

Název: Patogenetický význam nespecifické imunity u některých poruch imunitního systému (Akronym: Nespecifická imunita)

Investor: Masarykova univerzita, Patogenetický význam nespecifické imunity u některých poruch imunitního systému

NV16-31593A, projekt VaV

Název: Polymikrobiální biofilm jako komplexní mikrobiom v rámci léčby nozokomiálních infekcí

Citovat

KOTÁSKOVÁ, Iva, Vít SYROVÁTKA, Hana OBRUČOVÁ, Petra VÍDEŇSKÁ, Barbora ZWINSOVÁ, Veronika HOLÁ, Eva BLAŠTÍKOVÁ, Filip RŮŽIČKA a Tomáš FREIBERGER. Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines. Microorganisms. Basel: MDPI, 2021, roč. 9, č. 3, s. 1-23. ISSN 2076-2607. Dostupné z: https://dx.doi.org/10.3390/microorganisms9030669.

@article{1758116,
   author = {Kotásková, Iva and Syrovátka, Vít and Obručová, Hana and Vídeňská, Petra and Zwinsová, Barbora and Holá, Veronika and Blaštíková, Eva and Růžička, Filip and Freiberger, Tomáš},
   article_location = {Basel},
   article_number = {3},
   doi = {http://dx.doi.org/10.3390/microorganisms9030669},
   keywords = {Actinotignum; Actinobaculum; hydronephrosis; urinary catheter; ureteral stent; Double-J catheter; Propionimicrobium; Fusobacterium; urobiome; microbiome; colonisation},
   language = {eng},
   issn = {2076-2607},
   journal = {Microorganisms},
   title = {Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines},
   url = {https://www.mdpi.com/2076-2607/9/3/669},
   volume = {9},
   year = {2021}
}

TY  - JOUR
ID  - 1758116
AU  - Kotásková, Iva - Syrovátka, Vít - Obručová, Hana - Vídeňská, Petra - Zwinsová, Barbora - Holá, Veronika - Blaštíková, Eva - Růžička, Filip - Freiberger, Tomáš
PY  - 2021
TI  - Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines
JF  - Microorganisms
VL  - 9
IS  - 3
SP  - 1-23
EP  - 1-23
PB  - MDPI
SN  - 20762607
KW  - Actinotignum
KW  - Actinobaculum
KW  - hydronephrosis
KW  - urinary catheter
KW  - ureteral stent
KW  - Double-J catheter
KW  - Propionimicrobium
KW  - Fusobacterium
KW  - urobiome
KW  - microbiome
KW  - colonisation
UR  - https://www.mdpi.com/2076-2607/9/3/669
N2  - Actinotignum schaalii is an emerging, opportunistic pathogen and its connection to non-infectious diseases and conditions, such as prostate or bladder cancer, or chronic inflammation has been proposed. Here, we analyzed 297 urine, ureteral and urinary catheter samples from 128 patients by Polymerase Chain Reaction followed by Denaturing Gradient Gel Electrophoresis and Sequencing (PCR-DGGE-S), and culture, and 29 of these samples also by 16S rRNA Illumina sequencing, to establish A. schaalii’s prevalence in urinary tract-related samples, its relation to other bacteria, and its potential association with patients’ conditions and samples’ characteristics. A. schaalii-positive samples were significantly more diverse than A. schaalii negative and between-group diversity was higher than intra-group. Propionimicrobium lymphophilum, Fusobacterium nucleatum, Veillonella sp., Morganella sp., and Aerococcus sp. were significantly more often present in A. schaalii-positive samples; thus, we suggest these species are A. schaalii’s concomitants, while Enterobacter and Staphylococcaceae were more often identified in A. schaalii-negative samples; therefore, we propose A. schaalii and these species are mutually exclusive. Additionally, a significantly higher A. schaalii prevalence in patients with ureter stricture associated hydronephrosis (p = 0.020) was noted. We suggest that A. schaalii could be an early polybacterial biofilm colonizer, together with concomitant species, known for pro-inflammatory features.
ER  -

KOTÁSKOVÁ, Iva, Vít SYROVÁTKA, Hana OBRUČOVÁ, Petra VÍDEŇSKÁ, Barbora ZWINSOVÁ, Veronika HOLÁ, Eva BLAŠTÍKOVÁ, Filip RŮŽIČKA a Tomáš FREIBERGER. Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines. \textit{Microorganisms}. Basel: MDPI, 2021, roč.~9, č.~3, s.~1-23. ISSN~2076-2607. Dostupné z: https://dx.doi.org/10.3390/microorganisms9030669.

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