Assessment of Antimicrobic, Antivirotic and Cytotoxic Potential
of Alginate Beads Cross-Linked by Bivalent Ions for Vaginal
Administration

J 2021

Assessment of Antimicrobic, Antivirotic and Cytotoxic Potential of Alginate Beads Cross-Linked by Bivalent Ions for Vaginal Administration

PAVELKOVÁ, Miroslava, Jakub VYSLOUŽIL, Kateřina KUBOVÁ, Sylvie PAVLOKOVÁ, D. MOLINKOVA et. al.

Základní údaje

Originální název

Assessment of Antimicrobic, Antivirotic and Cytotoxic Potential of Alginate Beads Cross-Linked by Bivalent Ions for Vaginal Administration

Autoři

PAVELKOVÁ, Miroslava (203 Česká republika, domácí), Jakub VYSLOUŽIL (203 Česká republika, garant, domácí), Kateřina KUBOVÁ (203 Česká republika, domácí), Sylvie PAVLOKOVÁ (203 Česká republika, domácí), D. MOLINKOVA (203 Česká republika), V. CELER (203 Česká republika), A. PECHOVA (203 Česká republika), J. MASEK (203 Česká republika) a David VETCHÝ (203 Česká republika, domácí)

Vydání

Pharmaceutics, BASEL, Elsevier, 2021, 0939-6411

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.589

Kód RIV

RIV/00216224:14160/21:00121389

Organizační jednotka

Farmaceutická fakulta

DOI

http://dx.doi.org/10.3390/pharmaceutics13020165

UT WoS

000623016800001

Klíčová slova anglicky

ionic gelation; alginate; cross-linking; bivalent ions; cytotoxicity; antimicrobial activity; antiviral activity

Štítky

rivok, ÚFT

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 5. 2021 07:15, Mgr. Hana Hurtová

Anotace

V originále

Antimicrobial agent abuse poses a serious threat for future pharmacotherapy, including vaginal administration. The solution can be found in simple polymeric systems with inherent antimicrobial properties without the need to incorporate drugs, for instance alginate beads cross-linked by bivalent ions. The main goal of the presented study was to provide improvement on the well-documented cytotoxicity of Cu2+ cross-linked alginate. Alginate beads were prepared by external ionotropic gelation by cross-linking with Cu2+, Ca2+ and Zn2+ ions, separately and in mixtures. Morphological properties, swelling capacity, ion release and efficacy against the most common vaginal pathogens (C. albicans, E. coli, E. faecalis and virus strain-human herpesvirus type 1) were evaluated. The prepared particles (particle size 1455.68 +/- 18.71-1756.31 +/- 16.58 mu m) had very good sphericity (0.86 +/- 0.04-0.97 +/- 0.06). In mixture samples, Cu2+ hampered second ion loading, and was also released incompletely (18.75-44.8%) compared to the single ion Cu2+ sample (71.4%). Efficacy against the selected pathogens was confirmed in almost all samples. Although anticipating otherwise, ion mixture samples did not show betterment over a Cu2+ cross-linked sample in cytotoxicity-pathogen efficacy relation. However, the desired improvement was found in a single ion Zn2+ sample whose minimal inhibition concentrations against the pathogens (0.6-6.12 mM) were close to, or in the same mathematical order as, its toxic concentration of 50 (1.891 mM). In summary, these findings combined with alginate's biocompatibility and biodegradability give the combination solid potential in antimicrobial use.

Citovat

PAVELKOVÁ, Miroslava, Jakub VYSLOUŽIL, Kateřina KUBOVÁ, Sylvie PAVLOKOVÁ, D. MOLINKOVA, V. CELER, A. PECHOVA, J. MASEK a David VETCHÝ. Assessment of Antimicrobic, Antivirotic and Cytotoxic Potential of Alginate Beads Cross-Linked by Bivalent Ions for Vaginal Administration. Pharmaceutics. BASEL: Elsevier, 2021, roč. 13, č. 2, s. 1-20. ISSN 0939-6411. Dostupné z: https://dx.doi.org/10.3390/pharmaceutics13020165.

@article{1759161,
   author = {Pavelková, Miroslava and Vysloužil, Jakub and Kubová, Kateřina and Pavloková, Sylvie and Molinkova, D. and Celer, V. and Pechova, A. and Masek, J. and Vetchý, David},
   article_location = {BASEL},
   article_number = {2},
   doi = {http://dx.doi.org/10.3390/pharmaceutics13020165},
   keywords = {ionic gelation; alginate; cross-linking; bivalent ions; cytotoxicity; antimicrobial activity; antiviral activity},
   language = {eng},
   issn = {0939-6411},
   journal = {Pharmaceutics},
   title = {Assessment of Antimicrobic, Antivirotic and Cytotoxic Potential of Alginate Beads Cross-Linked by Bivalent Ions for Vaginal Administration},
   url = {https://pubmed.ncbi.nlm.nih.gov/33513747/},
   volume = {13},
   year = {2021}
}

TY  - JOUR
ID  - 1759161
AU  - Pavelková, Miroslava - Vysloužil, Jakub - Kubová, Kateřina - Pavloková, Sylvie - Molinkova, D. - Celer, V. - Pechova, A. - Masek, J. - Vetchý, David
PY  - 2021
TI  - Assessment of Antimicrobic, Antivirotic and Cytotoxic Potential of Alginate Beads Cross-Linked by Bivalent Ions for Vaginal Administration
JF  - Pharmaceutics
VL  - 13
IS  - 2
SP  - 1-20
EP  - 1-20
PB  - Elsevier
SN  - 09396411
KW  - ionic gelation
KW  - alginate
KW  - cross-linking
KW  - bivalent ions
KW  - cytotoxicity
KW  - antimicrobial activity
KW  - antiviral activity
UR  - https://pubmed.ncbi.nlm.nih.gov/33513747/
N2  - Antimicrobial agent abuse poses a serious threat for future pharmacotherapy, including vaginal administration. The solution can be found in simple polymeric systems with inherent antimicrobial properties without the need to incorporate drugs, for instance alginate beads cross-linked by bivalent ions. The main goal of the presented study was to provide improvement on the well-documented cytotoxicity of Cu2+ cross-linked alginate. Alginate beads were prepared by external ionotropic gelation by cross-linking with Cu2+, Ca2+ and Zn2+ ions, separately and in mixtures. Morphological properties, swelling capacity, ion release and efficacy against the most common vaginal pathogens (C. albicans, E. coli, E. faecalis and virus strain-human herpesvirus type 1) were evaluated. The prepared particles (particle size 1455.68 +/- 18.71-1756.31 +/- 16.58 mu m) had very good sphericity (0.86 +/- 0.04-0.97 +/- 0.06). In mixture samples, Cu2+ hampered second ion loading, and was also released incompletely (18.75-44.8%) compared to the single ion Cu2+ sample (71.4%). Efficacy against the selected pathogens was confirmed in almost all samples. Although anticipating otherwise, ion mixture samples did not show betterment over a Cu2+ cross-linked sample in cytotoxicity-pathogen efficacy relation. However, the desired improvement was found in a single ion Zn2+ sample whose minimal inhibition concentrations against the pathogens (0.6-6.12 mM) were close to, or in the same mathematical order as, its toxic concentration of 50 (1.891 mM). In summary, these findings combined with alginate's biocompatibility and biodegradability give the combination solid potential in antimicrobial use.
ER  -

PAVELKOVÁ, Miroslava, Jakub VYSLOUŽIL, Kateřina KUBOVÁ, Sylvie PAVLOKOVÁ, D. MOLINKOVA, V. CELER, A. PECHOVA, J. MASEK a David VETCHÝ. Assessment of Antimicrobic, Antivirotic and Cytotoxic Potential of Alginate Beads Cross-Linked by Bivalent Ions for Vaginal Administration. \textit{Pharmaceutics}. BASEL: Elsevier, 2021, roč.~13, č.~2, s.~1-20. ISSN~0939-6411. Dostupné z: https://dx.doi.org/10.3390/pharmaceutics13020165.

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