Urinary shedding of leptospires in palearctic bats

J 2021

Urinary shedding of leptospires in palearctic bats

SEIDLOVA, Veronika, Monika NEMCOVA, Jiří PIKULA, Tomáš BARTONIČKA, Astghik GHAZARYAN et. al.

Základní údaje

Originální název

Urinary shedding of leptospires in palearctic bats

Autoři

SEIDLOVA, Veronika (203 Česká republika), Monika NEMCOVA (203 Česká republika), Jiří PIKULA (203 Česká republika, garant), Tomáš BARTONIČKA (203 Česká republika, domácí), Astghik GHAZARYAN (51 Arménie), Tomas HEGER (203 Česká republika), Tomasz KOKUREWICZ (616 Polsko), Oleg L. ORLOV (643 Rusko), Sneha PATRA (203 Česká republika), Vladimir PIACEK (203 Česká republika), Frantisek TREML (203 Česká republika), Katerina ZUKALOVA (203 Česká republika) a Jan ZUKAL (203 Česká republika, domácí)

Vydání

Transboundary and Emerging Diseases, Hoboken, Wiley-Blackwell, 2021, 1865-1674

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.521

Kód RIV

RIV/00216224:14310/21:00121394

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1111/tbed.14011

UT WoS

000626853900001

Klíčová slova anglicky

Chiroptera; genetic classification; non invasive sampling; pathogenic Leptospira; prevalence; reservoirs; urine

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 22. 11. 2021 16:41, Mgr. Marie Šípková, DiS.

Anotace

V originále

Leptospirosis is a bacterial zoonotic infection of worldwide occurrence. Bats, like other mammalian reservoirs, may be long-term carriers that maintain endemicity of infection and shed viable leptospires in urine. Direct and/or indirect contact with these Leptospira shedders is the main risk factor as regards public health concern. However, knowledge about bat leptospirosis in the Palearctic Region, and in Europe in particular, is poor. We collected urine from 176 specimens of 11 bat species in the Czech Republic, Poland, Republic of Armenia and the Altai Region of Russia between 2014 and 2019. We extracted DNA from the urine samples to detect Leptospira spp. shedders using PCR amplification of the 16S rRNA and LipL32 genes. Four bat species (Barbastella barbastellus n = 1, Myotis bechsteinii n = 1, Myotis myotis n = 24 and Myotis nattereri n = 1) tested positive for Leptospira spp., with detected amplicons showing 100% genetic identity with pathogenic Leptospira interrogans. The site- and species-specific prevalence range was 0%-24.1% and 0%-20%, respectively. All bats sampled in the Republic of Armenia and Russia were negative. Given the circulation of pathogenic leptospires in strictly protected Palearctic bat species and their populations, non-invasive and non-lethal sampling of urine for molecular Leptospira spp. detection is recommended as a suitable surveillance and monitoring strategy. Moreover, our results should raise awareness of this potential disease risk among health professionals, veterinarians, chiropterologists and wildlife rescue workers handling bats, as well as speleologists and persons cleaning premises following bat infestation.

Citovat

SEIDLOVA, Veronika, Monika NEMCOVA, Jiří PIKULA, Tomáš BARTONIČKA, Astghik GHAZARYAN, Tomas HEGER, Tomasz KOKUREWICZ, Oleg L. ORLOV, Sneha PATRA, Vladimir PIACEK, Frantisek TREML, Katerina ZUKALOVA a Jan ZUKAL. Urinary shedding of leptospires in palearctic bats. Transboundary and Emerging Diseases. Hoboken: Wiley-Blackwell, 2021, roč. 68, č. 6, s. 3089-3095. ISSN 1865-1674. Dostupné z: https://dx.doi.org/10.1111/tbed.14011.

@article{1759380,
   author = {Seidlova, Veronika and Nemcova, Monika and Pikula, Jiří and Bartonička, Tomáš and Ghazaryan, Astghik and Heger, Tomas and Kokurewicz, Tomasz and Orlov, Oleg L. and Patra, Sneha and Piacek, Vladimir and Treml, Frantisek and Zukalova, Katerina and Zukal, Jan},
   article_location = {Hoboken},
   article_number = {6},
   doi = {http://dx.doi.org/10.1111/tbed.14011},
   keywords = {Chiroptera; genetic classification; non invasive sampling; pathogenic Leptospira; prevalence; reservoirs; urine},
   language = {eng},
   issn = {1865-1674},
   journal = {Transboundary and Emerging Diseases},
   title = {Urinary shedding of leptospires in palearctic bats},
   url = {https://doi.org/10.1111/tbed.14011},
   volume = {68},
   year = {2021}
}

TY  - JOUR
ID  - 1759380
AU  - Seidlova, Veronika - Nemcova, Monika - Pikula, Jiří - Bartonička, Tomáš - Ghazaryan, Astghik - Heger, Tomas - Kokurewicz, Tomasz - Orlov, Oleg L. - Patra, Sneha - Piacek, Vladimir - Treml, Frantisek - Zukalova, Katerina - Zukal, Jan
PY  - 2021
TI  - Urinary shedding of leptospires in palearctic bats
JF  - Transboundary and Emerging Diseases
VL  - 68
IS  - 6
SP  - 3089-3095
EP  - 3089-3095
PB  - Wiley-Blackwell
SN  - 18651674
KW  - Chiroptera
KW  - genetic classification
KW  - non invasive sampling
KW  - pathogenic Leptospira
KW  - prevalence
KW  - reservoirs
KW  - urine
UR  - https://doi.org/10.1111/tbed.14011
N2  - Leptospirosis is a bacterial zoonotic infection of worldwide occurrence. Bats, like other mammalian reservoirs, may be long-term carriers that maintain endemicity of infection and shed viable leptospires in urine. Direct and/or indirect contact with these Leptospira shedders is the main risk factor as regards public health concern. However, knowledge about bat leptospirosis in the Palearctic Region, and in Europe in particular, is poor. We collected urine from 176 specimens of 11 bat species in the Czech Republic, Poland, Republic of Armenia and the Altai Region of Russia between 2014 and 2019. We extracted DNA from the urine samples to detect Leptospira spp. shedders using PCR amplification of the 16S rRNA and LipL32 genes. Four bat species (Barbastella barbastellus n = 1, Myotis bechsteinii n = 1, Myotis myotis n = 24 and Myotis nattereri n = 1) tested positive for Leptospira spp., with detected amplicons showing 100% genetic identity with pathogenic Leptospira interrogans. The site- and species-specific prevalence range was 0%-24.1% and 0%-20%, respectively. All bats sampled in the Republic of Armenia and Russia were negative. Given the circulation of pathogenic leptospires in strictly protected Palearctic bat species and their populations, non-invasive and non-lethal sampling of urine for molecular Leptospira spp. detection is recommended as a suitable surveillance and monitoring strategy. Moreover, our results should raise awareness of this potential disease risk among health professionals, veterinarians, chiropterologists and wildlife rescue workers handling bats, as well as speleologists and persons cleaning premises following bat infestation.
ER  -

SEIDLOVA, Veronika, Monika NEMCOVA, Jiří PIKULA, Tomáš BARTONIČKA, Astghik GHAZARYAN, Tomas HEGER, Tomasz KOKUREWICZ, Oleg L. ORLOV, Sneha PATRA, Vladimir PIACEK, Frantisek TREML, Katerina ZUKALOVA a Jan ZUKAL. Urinary shedding of leptospires in palearctic bats. \textit{Transboundary and Emerging Diseases}. Hoboken: Wiley-Blackwell, 2021, roč.~68, č.~6, s.~3089-3095. ISSN~1865-1674. Dostupné z: https://dx.doi.org/10.1111/tbed.14011.

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