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@article{1759537,
author = {Kushkevych, Ivan and Hýžová, Blanka and Vítězová, Monika and Rittmann, Simon K.andM. R.},
article_location = {Basel},
article_number = {8},
doi = {http://dx.doi.org/10.3390/ijms22084007},
keywords = {microscopy; fluorescence microscopy; FISH; DAPI; Desulfovibrio; anaerobic microorganisms; habitats; SRB; SRP; SRM; sulfate reduction; identification; gut microbiota; IBD},
language = {eng},
issn = {1422-0067},
journal = {International Journal of Molecular Sciences},
title = {Microscopic Methods for Identification of Sulfate-Reducing Bacteria from Various Habitats},
url = {https://www.mdpi.com/1422-0067/22/8/4007},
volume = {22},
year = {2021}
}
TY - JOUR
ID - 1759537
AU - Kushkevych, Ivan - Hýžová, Blanka - Vítězová, Monika - Rittmann, Simon K.-M. R.
PY - 2021
TI - Microscopic Methods for Identification of Sulfate-Reducing Bacteria from Various Habitats
JF - International Journal of Molecular Sciences
VL - 22
IS - 8
SP - "4007"
EP - "4007"
PB - MDPI
SN - 14220067
KW - microscopy
KW - fluorescence microscopy
KW - FISH
KW - DAPI
KW - Desulfovibrio
KW - anaerobic microorganisms
KW - habitats
KW - SRB
KW - SRP
KW - SRM
KW - sulfate reduction
KW - identification
KW - gut microbiota
KW - IBD
UR - https://www.mdpi.com/1422-0067/22/8/4007
N2 - This paper is devoted to microscopic methods for the identification of sulfate-reducing bacteria (SRB). In this context, it describes various habitats, morphology and techniques used for the detection and identification of this very heterogeneous group of anaerobic microorganisms. SRB are present in almost every habitat on Earth, including freshwater and marine water, soils, sediments or animals. In the oil, water and gas industries, they can cause considerable economic losses due to their hydrogen sulfide production; in periodontal lesions and the colon of humans, they can cause health complications. Although the role of these bacteria in inflammatory bowel diseases is not entirely known yet, their presence is increased in patients and produced hydrogen sulfide has a cytotoxic effect. For these reasons, methods for the detection of these microorganisms were described. Apart from selected molecular techniques, including metagenomics, fluorescence microscopy was one of the applied methods. Especially fluorescence in situ hybridization (FISH) in various modifications was described. This method enables visual identification of SRB, determining their abundance and spatial distribution in environmental biofilms and gut samples.
ER -
KUSHKEVYCH, Ivan, Blanka HÝŽOVÁ, Monika VÍTĚZOVÁ a Simon K.-M. R. RITTMANN. Microscopic Methods for Identification of Sulfate-Reducing Bacteria from Various Habitats. \textit{International Journal of Molecular Sciences}. Basel: MDPI, 2021, roč.~22, č.~8, s.~''4007'', 27 s. ISSN~1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms22084007.
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