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@article{1766139, author = {Bernatík, Ondřej and Paclíková, Petra and Kotrbová, Anna and Bryja, Vítězslav and Čajánek, Lukáš}, article_location = {Lausanne}, article_number = {February}, doi = {http://dx.doi.org/10.3389/fcell.2021.623753}, keywords = {primary cilia; Wnt/beta-catenin; ciliogenesis; cell signaling; Wnt3a; RPE-1; HEK293; NIH3T3}, language = {eng}, issn = {2296-634X}, journal = {Frontiers in Cell and Developmental Biology}, title = {Primary Cilia Formation Does Not Rely on WNT/β-Catenin Signaling}, url = {https://doi.org/10.3389/fcell.2021.623753}, volume = {9}, year = {2021} }
TY - JOUR ID - 1766139 AU - Bernatík, Ondřej - Paclíková, Petra - Kotrbová, Anna - Bryja, Vítězslav - Čajánek, Lukáš PY - 2021 TI - Primary Cilia Formation Does Not Rely on WNT/β-Catenin Signaling JF - Frontiers in Cell and Developmental Biology VL - 9 IS - February SP - "623753" EP - "623753" PB - Frontiers Media S.A. SN - 2296634X KW - primary cilia KW - Wnt/beta-catenin KW - ciliogenesis KW - cell signaling KW - Wnt3a KW - RPE-1 KW - HEK293 KW - NIH3T3 UR - https://doi.org/10.3389/fcell.2021.623753 N2 - Primary cilia act as crucial regulators of embryo development and tissue homeostasis. They are instrumental for modulation of several signaling pathways, including Hedgehog, WNT, and TGF-β. However, gaps exist in our understanding of how cilia formation and function is regulated. Recent work has implicated WNT/β-catenin signaling pathway in the regulation of ciliogenesis, yet the results are conflicting. One model suggests that WNT/β-catenin signaling negatively regulates cilia formation, possibly via effects on cell cycle. In contrast, second model proposes a positive role of WNT/β-catenin signaling on cilia formation, mediated by the re-arrangement of centriolar satellites in response to phosphorylation of the key component of WNT/β-catenin pathway, β-catenin. To clarify these discrepancies, we investigated possible regulation of primary cilia by the WNT/β-catenin pathway in cell lines (RPE-1, NIH3T3, and HEK293) commonly used to study ciliogenesis. We used WNT3a to activate or LGK974 to block the pathway, and examined initiation of ciliogenesis, cilium length, and percentage of ciliated cells. We show that the treatment by WNT3a has no- or lesser inhibitory effect on cilia formation. Importantly, the inhibition of secretion of endogenous WNT ligands using LGK974 blocks WNT signaling but does not affect ciliogenesis. Finally, using knock-out cells for key WNT pathway components, namely DVL1/2/3, LRP5/6, or AXIN1/2 we show that neither activation nor deactivation of the WNT/β-catenin pathway affects the process of ciliogenesis. These results suggest that WNT/β-catenin-mediated signaling is not generally required for efficient cilia formation. In fact, activation of the WNT/β-catenin pathway in some systems seems to moderately suppress ciliogenesis. ER -
BERNATÍK, Ondřej, Petra PACLÍKOVÁ, Anna KOTRBOVÁ, Vítězslav BRYJA a Lukáš ČAJÁNEK. Primary Cilia Formation Does Not Rely on WNT/β-Catenin Signaling. \textit{Frontiers in Cell and Developmental Biology}. Lausanne: Frontiers Media S.A., 2021, roč.~9, February, s.~''623753'', 11 s. ISSN~2296-634X. Dostupné z: https://dx.doi.org/10.3389/fcell.2021.623753.
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